Restriction map for pF1KB7959
 (Linear) (Six-base) MAPSORT of: KIBB7959.seq  Check: 6280  from: 1  to: 792



KIBB7959.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:25  ..

AflIII A'CryG_T

Cuts at:      0     127     792 
   Size:        127     665
 
AleI CACnn'nnGTG

Cuts at:      0     119     752     792 
   Size:        119     633      40

  Fragments arranged by size: 

                 633     119      40
 
AlwNI CAG_nnn'CTG

Cuts at:      0     306     477     612     792 
   Size:        306     171     135     180

  Fragments arranged by size: 

                 306     180     171     135
 
ApoI r'AATT_y

Cuts at:      0     256     295     792 
   Size:        256      39     497

  Fragments arranged by size: 

                 497     256      39
 
AvaI C'yCGr_G

Cuts at:      0     550     792 
   Size:        550     242
 
BanI G'GyrC_C

Cuts at:      0     679     792 
   Size:        679     113
 
BanII G_rGCy'C

Cuts at:      0     232     792 
   Size:        232     560
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     740     792 
   Size:        740      52
 
BglI GCCn_nnn'nGGC

Cuts at:      0     218     792 
   Size:        218     574
 
BlpI GC'TnA_GC

Cuts at:      0      73     792 
   Size:         73     719
 
Bme1580I G_kGCm'C

Cuts at:      0     471     749     792 
   Size:        471     278      43

  Fragments arranged by size: 

                 471     278      43
 
BmgBI CAC'GTC

Cuts at:      0     117     792 
   Size:        117     675
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     576     792 
   Size:        576     216
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     313     792 
   Size:        313     479
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     563     593     792 
   Size:        563      30     199

  Fragments arranged by size: 

                 563     199      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     222     679     792 
   Size:        222     457     113

  Fragments arranged by size: 

                 457     222     113
 
BseYI C'CCAG_C

Cuts at:      0      90     245     599     705     792 
   Size:         90     155     354     106      87

  Fragments arranged by size: 

                 354     155     106      90      87
 
BsiEI CG_ry'CG

Cuts at:      0     218     282     792 
   Size:        218      64     510

  Fragments arranged by size: 

                 510     218      64
 
BsiHKAI G_wGCw'C

Cuts at:      0     116     500     792 
   Size:        116     384     292

  Fragments arranged by size: 

                 384     292     116
 
Bsp1286I G_dGCh'C

Cuts at:      0     116     232     471     500     749     792 
   Size:        116     116     239      29     249      43

  Fragments arranged by size: 

                 249     239     116     116      43      29
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     498     528     792 
   Size:        498      30     264

  Fragments arranged by size: 

                 498     264      30
 
BsrBI CCG'CTC

Cuts at:      0     547     792 
   Size:        547     245
 
BsrFI r'CCGG_y

Cuts at:      0     153     217     663     682     792 
   Size:        153      64     446      19     110

  Fragments arranged by size: 

                 446     153     110      64      19
 
BstEII G'GTnAC_C

Cuts at:      0     540     792 
   Size:        540     252
 
Bsu36I CC'TnA_GG

Cuts at:      0      23     792 
   Size:         23     769
 
BtgI C'CryG_G

Cuts at:      0     120     638     792 
   Size:        120     518     154

  Fragments arranged by size: 

                 518     154     120
 
DraIII CAC_nnn'GTG

Cuts at:      0     384     792 
   Size:        384     408
 
EaeI y'GGCC_r

Cuts at:      0     215     350     792 
   Size:        215     135     442

  Fragments arranged by size: 

                 442     215     135
 
EagI C'GGCC_G

Cuts at:      0     215     792 
   Size:        215     577
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      26     727     792 
   Size:         26     701      65

  Fragments arranged by size: 

                 701      65      26
 
EcoO109I rG'GnC_Cy

Cuts at:      0     179     474     609     792 
   Size:        179     295     135     183

  Fragments arranged by size: 

                 295     183     179     135
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      33     792 
   Size:         33     759
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      89     121     360     392     563     595     792 
   Size:         89      32     239      32     171      32     197

  Fragments arranged by size: 

                 239     197     171      89      32      32      32
 
MluI A'CGCG_T

Cuts at:      0     127     792 
   Size:        127     665
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     594     767     792 
   Size:        594     173      25

  Fragments arranged by size: 

                 594     173      25
 
MscI TGG'CCA

Cuts at:      0     352     792 
   Size:        352     440
 
MslI CAynn'nnrTG

Cuts at:      0      11     119     421     752     792 
   Size:         11     108     302     331      40

  Fragments arranged by size: 

                 331     302     108      40      11
 
MspA1I CmG'CkG

Cuts at:      0      43     594     603     792 
   Size:         43     551       9     189

  Fragments arranged by size: 

                 551     189      43       9
 
NaeI GCC'GGC

Cuts at:      0     155     219     665     792 
   Size:        155      64     446     127

  Fragments arranged by size: 

                 446     155     127      64
 
NgoMIV G'CCGG_C

Cuts at:      0     153     217     663     792 
   Size:        153      64     446     129

  Fragments arranged by size: 

                 446     153     129      64
 
PpuMI rG'GwC_Cy

Cuts at:      0     179     474     792 
   Size:        179     295     318

  Fragments arranged by size: 

                 318     295     179
 
PstI C_TGCA'G

Cuts at:      0      56     608     650     792 
   Size:         56     552      42     142

  Fragments arranged by size: 

                 552     142      56      42
 
PvuII CAG'CTG

Cuts at:      0     594     603     792 
   Size:        594       9     189

  Fragments arranged by size: 

                 594     189       9
 
SanDI GG'GwC_CC

Cuts at:      0     179     792 
   Size:        179     613
 
SfcI C'TryA_G

Cuts at:      0      52     604     646     792 
   Size:         52     552      42     146

  Fragments arranged by size: 

                 552     146      52      42
 
SmlI C'TyrA_G

Cuts at:      0     328     792 
   Size:        328     464
 
StyI C'CwwG_G

Cuts at:      0     101     728     792 
   Size:        101     627      64

  Fragments arranged by size: 

                 627     101      64
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     775     792 
   Size:        775      17
 
TatI w'GTAC_w

Cuts at:      0     389     792 
   Size:        389     403
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     580     612     792 
   Size:        580      32     180

  Fragments arranged by size: 

                 580     180      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     772     792 
   Size:        772      20
 
XmnI GAAnn'nnTTC

Cuts at:      0      16     792 
   Size:         16     776
 

 Enzymes that do cut:

   AflIII     AleI    AlwNI     ApoI     AvaI     BanI    BanII     BbsI
     BglI     BlpI Bme1580I    BmgBI     BplI    BpuEI    BsaXI    BseRI
    BseYI    BsiEI  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrFI   BstEII
   Bsu36I     BtgI   DraIII     EaeI     EagI    EcoNI EcoO109I     FalI
    Hin4I     MluI     MmeI     MscI     MslI   MspA1I     NaeI   NgoMIV
    PpuMI     PstI    PvuII    SanDI     SfcI     SmlI     StyI    TaqII
     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI    BglII     BmrI     BmtI     BpmI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI     BsgI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI
    BstXI    BstYI  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI
     DrdI     EarI     EciI  EcoICRI  Eco57MI    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI  Tth111I     XbaI     XhoI     XmaI     ZraI
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