Restriction map for pF1KB7957
##################################
# NO CUT ENZYMES (  145 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AviII       AvrII       BanI        BbeI        BbsI        
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaBI       BsaHI       BseAI       BsiHKAI     
BsiWI       BsmI        BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BsrFI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      Eam1105I    Eco47III    
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       Hin1I       
HincII      HindII      HpaI        KasI        KpnI        
KspI        MaeI        MamI        MfeI        MluI        
MroI        MseI        MspA1I      MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmeI        PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspI        StuI        SwaI        TaqI        
TfiI        Tru9I       Tsp509I     Tth111I     TthHB8I     
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccII         485  CGCG                 CG^CG
AfaI          474  GTAC                 GT^AC
AlwNI         508  CAGGCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaI          163  CTCGGG               C^[CT]CG[GA]G
BalI          260  TGGCCA               TGG^CCA
BamHI         446  GGATCC               G^GATCC
BanII         565  GAGCCC               G[GA]GC[CT]^C
BbrPI         256  CACGTG               CAC^GTG
BmyI          565  GAGCCC               G[AGT]GC[ACT]^C
BsaAI         256  CACGTG               [CT]AC^GT[GA]
BsaI          718  GGTCTC               GGTCTC(1/5)
BsaWI         428  ACCGGA               [AT]^CCGG[AT]
BseRI         697  GAGGAG               GAGGAG(10/8)
BsgI          628  GTGCAG               GTGCAG(16/14)
BsiEI         143  CGGCCG               CG[GA][CT]^CG
BsmBI         183  CGTCTC               CGTCTC(1/5)
BsoBI         163  CTCGGG               C^[CT]CG[GA]G
Bsp1286I      565  GAGCCC               G[AGT]GC[ACT]^C
Bsp1407I      473  TGTACA               T^GTACA
BspMI         133  GCAGGT               ACCTGC(4/8)
BsrBI         592  CCGCTC               CCGCTC(-3/-3)
BsrGI         473  TGTACA               T^GTACA
BstUI         485  CGCG                 CG^CG
BstYI         446  GGATCC               [GA]^GATC[CT]
CfoI          486  GCGC                 GCG^C
EagI          143  CGGCCG               C^GGCCG
EarI          704  CTCTTC               CTCTTC(1/4)
EclXI         143  CGGCCG               C^GGCCG
Eco52I        143  CGGCCG               C^GGCCG
EcoNI         555  CCTACTCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI          591  CCCGC                CCCGC(4/6)
HgaI          251  GCGTC                GACGC(5/10)
HhaI          486  GCGC                 GCG^C
HinP1I        486  GCGC                 G^CGC
HindIII        42  AAGCTT               A^AGCTT
Ksp632I       704  CTCTTC               CTCTTC(1/4)
MaeII         257  ACGT                 A^CGT
MflI          446  GGATCC               [GA]^GATC[CT]
MluNI         260  TGGCCA               TGG^CCA
MscI          260  TGGCCA               TGG^CCA
MslI          105  CACAGCCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          485  CGCG                 CG^CG
MwoI          404  GCCTCAGCCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflMI         507  CCAGGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         256  CACGTG               CAC^GTG
PmlI          256  CACGTG               CAC^GTG
PpuMI          69  GGGACCT              [GA]G^G[AT]CC[CT]
PshAI         177  GACCATCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          577  CTGCAG               CTGCA^G
RsaI          474  GTAC                 GT^AC
SspBI         473  TGTACA               T^GTACA
Van91I        507  CCAGGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          248  CCAGCGTCCACGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         446  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

BbvCI         405  CCTCAGC              CCTCAGC(-5/-2)
              732  CCTCAGC             
BbvI          551  GCTGC                GCAGC(8/12)
              685  GCTGC               
BpmI          505  CTCCAG               CTGGAG(16/14)
              341  CTGGAG              
BsrI          200  ACTGG                ACTGG(1/-1)
              647  ACTGG               
BstXI         104  CCACAGCCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              640  CCACTCAACTGG        
DpnII         432  GATC                 ^GATC
              447  GATC                
DrdI          207  GACGGTGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              757  GACGAGGCTGTC        
EaeI          143  CGGCCG               [CT]^GGCC[GA]
              260  TGGCCA              
HphI          481  TCACC                GGTGA(8/7)
              491  TCACC               
MboI          432  GATC                 ^GATC
              447  GATC                
MboII          20  GAAGA                GAAGA(8/7)
              705  TCTTC               
NcoI           80  CCATGG               C^CATGG
              273  CCATGG              
NdeII         432  GATC                 ^GATC
              447  GATC                
NlaIII         81  CATG                 CATG^
              274  CATG                
Sau3AI        432  GATC                 ^GATC
              447  GATC                
SfaNI         778  GATGC                GCATC(5/9)
              418  GCATC               
SfcI          441  CTACAG               C^T[GA][CT]AG
              577  CTGCAG              
SmlI           45  CTTGAG               C^T[CT][GA]AG
              559  CTCAAG              
TseI          551  GCTGC                G^C[AT]GC
              685  GCTGC               
Tsp45I         76  GTGAC                ^GT[GC]AC
              622  GTGAC               
TspRI         451  CACTG                CA[GC]TG[ACGT][ACGT]^
              711  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   38 enzymes) #
#########################################

AciI        AluI        AlwI        AvaII       BcnI        
BsaJI       BsiYI       BslI        BsmAI       BsmFI       
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DdeI        DraII       DsaI        EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       ItaI        MaeIII      MnlI        
MspI        MvaI        NciI        NlaIV       PleI        
Sau96I      ScrFI       StyI        
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