Restriction map for pF1KB7957
 (Linear) (Six-base) MAPSORT of: KIBB7957.seq  Check: 6953  from: 1  to: 783



KIBB7957.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     124     783 
   Size:        124     659
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     239     701     783 
   Size:        239     462      82

  Fragments arranged by size: 

                 462     239      82
 
AleI CACnn'nnGTG

Cuts at:      0     109     783 
   Size:        109     674
 
AlwNI CAG_nnn'CTG

Cuts at:      0     513     783 
   Size:        513     270
 
AvaI C'yCGr_G

Cuts at:      0     163     783 
   Size:        163     620
 
BamHI G'GATC_C

Cuts at:      0     446     783 
   Size:        446     337
 
BanII G_rGCy'C

Cuts at:      0     569     783 
   Size:        569     214
 
BbvCI CC'TCA_GC

Cuts at:      0     406     733     783 
   Size:        406     327      50

  Fragments arranged by size: 

                 406     327      50
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     783 
   Size:        783
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     362     490     783 
   Size:        362     128     293

  Fragments arranged by size: 

                 362     293     128
 
Bpu10I CC'TnA_GC

Cuts at:      0     295     406     733     783 
   Size:        295     111     327      50

  Fragments arranged by size: 

                 327     295     111      50
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      66     544     783 
   Size:         66     478     239

  Fragments arranged by size: 

                 478     239      66
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     724     783 
   Size:        724      59
 
BsaAI yAC'GTr

Cuts at:      0     258     783 
   Size:        258     525
 
BsaWI w'CCGG_w

Cuts at:      0     428     783 
   Size:        428     355
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     712     783 
   Size:        712      71
 
BseYI C'CCAG_C

Cuts at:      0      13     247     783 
   Size:         13     234     536

  Fragments arranged by size: 

                 536     234      13
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     649     783 
   Size:        649     134
 
BsiEI CG_ry'CG

Cuts at:      0     146     783 
   Size:        146     637
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     189     783 
   Size:        189     594
 
Bsp1286I G_dGCh'C

Cuts at:      0     569     783 
   Size:        569     214
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     124     783 
   Size:        124     659
 
BsrBI CCG'CTC

Cuts at:      0     594     783 
   Size:        594     189
 
BsrGI T'GTAC_A

Cuts at:      0     473     783 
   Size:        473     310
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     111     647     783 
   Size:        111     536     136

  Fragments arranged by size: 

                 536     136     111
 
BstYI r'GATC_y

Cuts at:      0     446     783 
   Size:        446     337
 
BtgI C'CryG_G

Cuts at:      0      80     110     273     783 
   Size:         80      30     163     510

  Fragments arranged by size: 

                 510     163      80      30
 
BtsI GCAGTG_nn'

Cuts at:      0     450     783 
   Size:        450     333
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     213     763     783 
   Size:        213     550      20

  Fragments arranged by size: 

                 550     213      20
 
EaeI y'GGCC_r

Cuts at:      0     143     260     783 
   Size:        143     117     523

  Fragments arranged by size: 

                 523     143     117
 
EagI C'GGCC_G

Cuts at:      0     143     783 
   Size:        143     640
 
EarI CTCTTCn'nnn_

Cuts at:      0     710     783 
   Size:        710      73
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     391     553     783 
   Size:        391     162     230

  Fragments arranged by size: 

                 391     230     162
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     239     362     490     701     783 
   Size:        239     123     128     211      82

  Fragments arranged by size: 

                 239     211     128     123      82
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     559     783 
   Size:        559     224
 
EcoO109I rG'GnC_Cy

Cuts at:      0      70     291     347     510     783 
   Size:         70     221      56     163     273

  Fragments arranged by size: 

                 273     221     163      70      56
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     511     543     783 
   Size:        511      32     240

  Fragments arranged by size: 

                 511     240      32
 
HindIII A'AGCT_T

Cuts at:      0      42     783 
   Size:         42     741
 
MscI TGG'CCA

Cuts at:      0     262     783 
   Size:        262     521
 
MslI CAynn'nnrTG

Cuts at:      0     109     783 
   Size:        109     674
 
NcoI C'CATG_G

Cuts at:      0      80     273     783 
   Size:         80     193     510

  Fragments arranged by size: 

                 510     193      80
 
PasI CC'CwG_GG

Cuts at:      0     117     783 
   Size:        117     666
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     513     783 
   Size:        513     270
 
PfoI T'CCnGG_A

Cuts at:      0     359     722     783 
   Size:        359     363      61

  Fragments arranged by size: 

                 363     359      61
 
PmlI CAC'GTG

Cuts at:      0     258     783 
   Size:        258     525
 
PpuMI rG'GwC_Cy

Cuts at:      0      70     783 
   Size:         70     713
 
PshAI GACnn'nnGTC

Cuts at:      0     181     783 
   Size:        181     602
 
PstI C_TGCA'G

Cuts at:      0     581     783 
   Size:        581     202
 
SfcI C'TryA_G

Cuts at:      0     441     577     783 
   Size:        441     136     206

  Fragments arranged by size: 

                 441     206     136
 
SmlI C'TyrA_G

Cuts at:      0      45     559     783 
   Size:         45     514     224

  Fragments arranged by size: 

                 514     224      45
 
StyI C'CwwG_G

Cuts at:      0      60      80     273     783 
   Size:         60      20     193     510

  Fragments arranged by size: 

                 510     193      60      20
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     266     783 
   Size:        266     517
 
TatI w'GTAC_w

Cuts at:      0     473     783 
   Size:        473     310
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     255     783 
   Size:        255     528
 

 Enzymes that do cut:

     AarI     AcuI     AleI    AlwNI     AvaI    BamHI    BanII    BbvCI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaWI    BseRI
    BseYI     BsgI    BsiEI    BsmBI Bsp1286I    BspMI    BsrBI    BsrGI
    BstXI    BstYI     BtgI     BtsI     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI    EcoNI EcoO109I    Hin4I  HindIII     MscI     MslI
     NcoI     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI     PstI
     SfcI     SmlI     StyI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI     BanI     BbeI     BbsI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI    BsaBI    BsaHI    BsaXI  BsiHKAI    BsiWI     BsmI    BspEI
    BspHI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII  EcoICRI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PciI     PmeI     PpiI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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