Restriction map for pF1KB7956
##################################
# NO CUT ENZYMES (  143 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BglII       
BlnI        BpuAI       BsaAI       BsaBI       BsaHI       
BseAI       BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmI        BsoBI       BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstXI       BstZ17I     Bsu36I      Cfr10I      
ClaI        DraI        DrdI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoRI       EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       Hin1I       
HpaI        KasI        KpnI        Ksp632I     KspI        
MamI        MfeI        MluNI       MroI        MscI        
MseI        MunI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
SacI        SacII       SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SspI        
SwaI        Tru9I       Tth111I     XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccI          953  GTCGAC               GT^[AC][GT]AC
AccII          30  CGCG                 CG^CG
AflIII         29  ACGCGT               A^C[GA][CT]GT
ApaI          359  GGGCCC               GGGCC^C
Asp700I       207  GAAGCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI          67  GGATCC               G^GATCC
BglI          307  GCCCCCAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          823  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      823  GCTCAGC              GC^T[ACGT]AGC
BsaI         1042  GAGACC               GGTCTC(1/5)
BsgI          758  CTGCAC               GTGCAG(16/14)
BsmAI        1042  GAGAC                GTCTC(1/5)
Bsp1407I      532  TGTACA               T^GTACA
BsrGI         532  TGTACA               T^GTACA
BstUI          30  CGCG                 CG^CG
CelII         823  GCTCAGC              GC^T[ACGT]AGC
CfoI         1152  GCGC                 GCG^C
CpoI         1159  CGGTCCG              CG^G[AT]CCG
DraIII        988  CACGGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          165  CCGTGG               C^C[GA][CT]GG
EcoRV          52  GATATC               GAT^ATC
HhaI         1152  GCGC                 GCG^C
HinP1I       1152  GCGC                 G^CGC
HincII        953  GTCGAC               GT[CT]^[GA]AC
HindII        953  GTCGAC               GT[CT]^[GA]AC
HindIII       208  AAGCTT               A^AGCTT
MaeII        1025  ACGT                 A^CGT
MluI           29  ACGCGT               A^CGCGT
MslI         1171  CATGCAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        835  CAGCTG               C[AC]G^C[GT]G
MvnI           30  CGCG                 CG^CG
NspI         1170  GCATGC               [GA]CATG^[CT]
PpuMI         647  GGGTCCT              [GA]G^G[AT]CC[CT]
PstI          838  CTGCAG               CTGCA^G
PvuII         835  CAGCTG               CAG^CTG
RsrII        1159  CGGTCCG              CG^G[AT]CCG
SalI          953  GTCGAC               G^TCGAC
SphI         1170  GCATGC               GCATG^C
SspBI         532  TGTACA               T^GTACA
StuI          399  AGGCCT               AGG^CCT
XmnI          207  GAAGCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

BanI          444  GGTGCC               G^G[CT][GA]CC
              511  GGCACC              
BfaI          119  CTAG                 C^TAG
              418  CTAG                
BseRI         574  GAGGAG               GAGGAG(10/8)
              640  GAGGAG              
BspMI          20  ACCTGC               ACCTGC(4/8)
              620  ACCTGC              
BstYI           3  GGATCT               [GA]^GATC[CT]
               67  GGATCC              
EcoNI         627  CCTCACCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              651  CCTCAAGAAGG         
HphI          618  TCACC                GGTGA(8/7)
              629  TCACC               
MaeI          119  CTAG                 C^TAG
              418  CTAG                
MflI            3  GGATCT               [GA]^GATC[CT]
               67  GGATCC              
PflMI         789  CCAGGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              958  CCAGAAGCTGG         
SfcI          124  CTACAG               C^T[GA][CT]AG
              838  CTGCAG              
SmlI          652  CTCAAG               C^T[CT][GA]AG
             1117  CTTGAG              
TaqI           61  TCGA                 T^CGA
              954  TCGA                
Tsp45I        260  GTGAC                ^GT[GC]AC
              994  GTGAC               
Tsp509I       773  AATT                 ^AATT
              849  AATT                
TthHB8I        61  TCGA                 T^CGA
              954  TCGA                
Van91I        789  CCAGGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              958  CCAGAAGCTGG         
XhoII           3  GGATCT               [GA]^GATC[CT]
               67  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AvaII       BanII       BbvI        BmyI        BpmI        
BsaJI       BsaWI       BsiYI       BslI        BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        DpnII       DraII       
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HgaI        HinfI       HpaII       
ItaI        MaeIII      MboI        MboII       MnlI        
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       StyI        TfiI        TseI        
TspRI       
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