Restriction map for pF1KB7947
 (Circular) (Six-base) MAPSORT of: pF1KB7947.seq  Check: 8217  from: 1  to: 3875



pF1KB7947  3875 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:25  ..

AccI GT'mk_AC

Cuts at:    913     913 
   Size:       3875
 
Acc65I G'GTAC_C

Cuts at:    891     891 
   Size:       3875
 
AclI AA'CG_TT

Cuts at:   3715    3715 
   Size:       3875
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1700    2132    2879    3147    1700 
   Size:        432     747     268    2428

  Fragments arranged by size: 

                2428     747     432     268
 
AflIII A'CryG_T

Cuts at:   1227    2331    3308    1227 
   Size:       1104     977    1794

  Fragments arranged by size: 

                1794    1104     977
 
AgeI A'CCGG_T

Cuts at:   2263    2263 
   Size:       3875
 
AlwNI CAG_nnn'CTG

Cuts at:    128     157    1180    2747     128 
   Size:         29    1023    1567    1256

  Fragments arranged by size: 

                1567    1256    1023      29
 
ApaLI G'TGCA_C

Cuts at:   2645    2645 
   Size:       3875
 
ApoI r'AATT_y

Cuts at:    879    3228    3484     879 
   Size:       2349     256    1270

  Fragments arranged by size: 

                2349    1270     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3875
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3875
 
AvaI C'yCGr_G

Cuts at:    895     895 
   Size:       3875
 
BamHI G'GATC_C

Cuts at:    111     900     111 
   Size:        789    3086
 
BanI G'GyrC_C

Cuts at:    891    1305    1553    1588     891 
   Size:        414     248      35    3178

  Fragments arranged by size: 

                3178     414     248      35
 
BanII G_rGCy'C

Cuts at:    889    1919     889 
   Size:       1030    2845
 
BbeI G_GCGC'C

Cuts at:   1309    1557    1309 
   Size:        248    3627
 
BciVI GTATCCnnnnn_n'

Cuts at:   1773    2534    3875    1773 
   Size:        761    1341    1773

  Fragments arranged by size: 

                1773    1341     761
 
BclI T'GATC_A

Cuts at:   1395    1395 
   Size:       3875
 
BglII A'GATC_T

Cuts at:   1390    3427    1390 
   Size:       2037    1838
 
BlpI GC'TnA_GC

Cuts at:    987     987 
   Size:       3875
 
Bme1580I G_kGCm'C

Cuts at:   1500    1593    2649    1500 
   Size:         93    1056    2726

  Fragments arranged by size: 

                2726    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1087    1168    1503    3647    1087 
   Size:         81     335    2144    1315

  Fragments arranged by size: 

                2144    1315     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2242    2242 
   Size:       3875
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    127     685     127 
   Size:        558    3317
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1051    1930    2422    2720    1051 
   Size:        879     492     298    2206

  Fragments arranged by size: 

                2206     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3579    3579 
   Size:       3875
 
BsaAI yAC'GTr

Cuts at:   1228    1228 
   Size:       3875
 
BsaBI GATnn'nnATC

Cuts at:      6    1389    3426       6 
   Size:       1383    2037     455

  Fragments arranged by size: 

                2037    1383     455
 
BsaHI Gr'CG_yC

Cuts at:    407    1306    1554    2256    3752     407 
   Size:        899     248     702    1496     530

  Fragments arranged by size: 

                1496     899     702     530     248
 
BsaWI w'CCGG_w

Cuts at:   1064    1585    2263    2537    2684    3095    1064 
   Size:        521     678     274     147     411    1844

  Fragments arranged by size: 

                1844     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    658     688     840     870     658 
   Size:         30     152      30    3663

  Fragments arranged by size: 

                3663     152      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    150     352     150 
   Size:        202    3673
 
BseYI C'CCAG_C

Cuts at:    150    1300    2058    2635    3131     150 
   Size:       1150     758     577     496     894

  Fragments arranged by size: 

                1150     894     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1417    1463    2671     104 
   Size:       1313      46    1208    1308

  Fragments arranged by size: 

                1313    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    344     526     889    1667    1857    2649     344 
   Size:        182     363     778     190     792    1570

  Fragments arranged by size: 

                1570     792     778     363     190     182
 
Bsp1286I G_dGCh'C

Cuts at:    344     526     889    1500    1593    1667    1857    1919    2649 
   Size:        182     363     611      93      74     190      62     730

Cuts at:   2649     344 
   Size:       1570

  Fragments arranged by size: 

                1570     730     611     363     190     182      93      74
                  62
 
BspEI T'CCGG_A

Cuts at:   3095    3095 
   Size:       3875
 
BspHI T'CATG_A

Cuts at:   3051    3051 
   Size:       3875
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    925    1139    1441    1822     925 
   Size:        214     302     381    2978

  Fragments arranged by size: 

                2978     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2167    2221    3705    3870      50 
   Size:       2117      54    1484     165      55

  Fragments arranged by size: 

                2117    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    201    1787     201 
   Size:       1586    2289
 
BsrFI r'CCGG_y

Cuts at:   1873    2054    2263    1873 
   Size:        181     209    3485

  Fragments arranged by size: 

                3485     209     181
 
BssSI C'ACGA_G

Cuts at:   2146    2504    2146 
   Size:        358    3517
 
BstAPI GCAn_nnn'nTGC

Cuts at:    676    1252     676 
   Size:        576    3299
 
BstBI TT'CG_AA

Cuts at:    174    2236     174 
   Size:       2062    1813
 
BstXI CCAn_nnnn'nTGG

Cuts at:    453     468     453 
   Size:         15    3860
 
BstYI r'GATC_y

Cuts at:      1     111     900    1367    1390    1725    1971    2983    3069 
   Size:        110     789     467      23     335     246    1012      86

Cuts at:   3069    3081    3427       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     789     467     449     346     335     246     110
                  86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    204     833     204 
   Size:        629    3246
 
BtgI C'CryG_G

Cuts at:    107     116     107 
   Size:          9    3866
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    515    1825    2006    2169     515 
   Size:       1310     181     163    2221

  Fragments arranged by size: 

                2221    1310     181     163
 
BtsI GCAGTG_nn'

Cuts at:    524    1118     524 
   Size:        594    3281
 
DraI TTT'AAA

Cuts at:    874     874 
   Size:       3875
 
DrdI GACnn_nn'nnGTC

Cuts at:   1581    2439    1581 
   Size:        858    3017
 
EaeI y'GGCC_r

Cuts at:    119    1460    1634    2025    2052    3397     119 
   Size:       1341     174     391      27    1345     597

  Fragments arranged by size: 

                1345    1341     597     391     174      27
 
EagI C'GGCC_G

Cuts at:   1460    1460 
   Size:       3875
 
EarI CTCTTCn'nnn_

Cuts at:   1898    2108    1898 
   Size:        210    3665
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    573    2083    2393    2539    3685     573 
   Size:       1510     310     146    1146     763

  Fragments arranged by size: 

                1510    1146     763     310     146
 
EcoICRI GAG'CTC

Cuts at:    887     887 
   Size:       3875
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    127     685    1700    2132    2879    3147     127 
   Size:        558    1015     432     747     268     855

  Fragments arranged by size: 

                1015     855     747     558     432     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:    331     331 
   Size:       3875
 
EcoO109I rG'GnC_Cy

Cuts at:    200     828    1014     200 
   Size:        628     186    3061

  Fragments arranged by size: 

                3061     628     186
 
EcoRI G'AATT_C

Cuts at:    879     879 
   Size:       3875
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1894    1894 
   Size:       3875
 
FspI TGC'GCA

Cuts at:   1656    1656 
   Size:       3875
 
HaeII r_GCGC'y

Cuts at:    747    1309    1557    2579    3558     747 
   Size:        562     248    1022     979    1064

  Fragments arranged by size: 

                1064    1022     979     562     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    532     564     829     861    1717    1747    1749    1779     532 
   Size:         32     265      32     856      30       2      30    2628

  Fragments arranged by size: 

                2628     856     265      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    914     914 
   Size:       3875
 
KasI G'GCGC_C

Cuts at:   1305    1553    1305 
   Size:        248    3627
 
KpnI G_GTAC'C

Cuts at:    895     895 
   Size:       3875
 
MluI A'CGCG_T

Cuts at:   3308    3308 
   Size:       3875
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2546    2730    2546 
   Size:        184    3691
 
MscI TGG'CCA

Cuts at:   1636    1636 
   Size:       3875
 
MslI CAynn'nnrTG

Cuts at:   1864    1991    1864 
   Size:        127    3748
 
MspA1I CmG'CkG

Cuts at:    118     128     654     986    1300    1660    2673    2918     118 
   Size:         10     526     332     314     360    1013     245    1075

  Fragments arranged by size: 

                1075    1013     526     360     332     314     245      10
            
 
NaeI GCC'GGC

Cuts at:   2056    2056 
   Size:       3875
 
NarI GG'CG_CC

Cuts at:   1306    1554    1306 
   Size:        248    3627
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3875
 
NgoMIV G'CCGG_C

Cuts at:   2054    2054 
   Size:       3875
 
NspI r_CATG'y

Cuts at:    928    1428    2335     928 
   Size:        500     907    2468

  Fragments arranged by size: 

                2468     907     500
 
PciI A'CATG_T

Cuts at:   2331    2331 
   Size:       3875
 
PmeI GTTT'AAAC

Cuts at:    874     874 
   Size:       3875
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3014    3046    3014 
   Size:         32    3843
 
PpuMI rG'GwC_Cy

Cuts at:    828     828 
   Size:       3875
 
PstI C_TGCA'G

Cuts at:    922    1607     922 
   Size:        685    3190
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3875
 
PvuII CAG'CTG

Cuts at:    128     654    1300    1660     128 
   Size:        526     646     360    2343

  Fragments arranged by size: 

                2343     646     526     360
 
RsrII CG'GwC_CG

Cuts at:   2070    2070 
   Size:       3875
 
SacI G_AGCT'C

Cuts at:    889     889 
   Size:       3875
 
SacII CC_GC'GG

Cuts at:    119     119 
   Size:       3875
 
SalI G'TCGA_C

Cuts at:    912     912 
   Size:       3875
 
SapI GCTCTTCn'nnn_

Cuts at:   1898    2108    1898 
   Size:        210    3665
 
SbfI CC_TGCA'GG

Cuts at:    922     922 
   Size:       3875
 
ScaI AGT'ACT

Cuts at:   3392    3392 
   Size:       3875
 
SfcI C'TryA_G

Cuts at:     33     918    1603    2596    2787      33 
   Size:        885     685     993     191    1121

  Fragments arranged by size: 

                1121     993     885     685     191
 
SfoI GGC'GCC

Cuts at:   1307    1555    1307 
   Size:        248    3627
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3875
 
SmaI CCC'GGG

Cuts at:    897     897 
   Size:       3875
 
SmlI C'TyrA_G

Cuts at:   1030    1945    2437    2699    1030 
   Size:        915     492     262    2206

  Fragments arranged by size: 

                2206     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3875
 
SphI G_CATG'C

Cuts at:    928    1428     928 
   Size:        500    3375
 
StyI C'CwwG_G

Cuts at:    107     446     644    1009    1363     107 
   Size:        339     198     365     354    2619

  Fragments arranged by size: 

                2619     365     354     339     198
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1458    1591    2050    2259    1458 
   Size:        133     459     209    3074

  Fragments arranged by size: 

                3074     459     209     133
 
TatI w'GTAC_w

Cuts at:   3390    3390 
   Size:       3875
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    840     872    3392    3424     840 
   Size:         32    2520      32    1291

  Fragments arranged by size: 

                2520    1291      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1672    1672 
   Size:       3875
 
XbaI T'CTAG_A

Cuts at:    906     906 
   Size:       3875
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    158    1370     158 
   Size:       1212    2663
 
XmaI C'CCGG_G

Cuts at:    895     895 
   Size:       3875
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PciI
     PmeI     PpiI    PpuMI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI
     BglI    BmgBI     BmtI   Bpu10I     BsgI    BsiWI     BsmI    BsmBI
    BsrGI   BssHII   BstEII  BstZ17I     ClaI    CspCI   DraIII    EcoRV
     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmlI    PshAI
     PsiI   PspOMI    PspXI     PsrI    SanDI    SexAI     SfiI    SgrAI
    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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