Restriction map for pF1KB7940
 (Linear) (Six-base) MAPSORT of: KIBB7940.seq  Check: 4873  from: 1  to: 720



KIBB7940.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:25  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     352     720 
   Size:         46     306     368

  Fragments arranged by size: 

                 368     306      46
 
ApaLI G'TGCA_C

Cuts at:      0     511     720 
   Size:        511     209
 
AvaI C'yCGr_G

Cuts at:      0     419     720 
   Size:        419     301
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     166     455     720 
   Size:        166     289     265

  Fragments arranged by size: 

                 289     265     166
 
BglII A'GATC_T

Cuts at:      0     327     720 
   Size:        327     393
 
Bme1580I G_kGCm'C

Cuts at:      0     515     720 
   Size:        515     205
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     165     720 
   Size:        165     555
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     155     720 
   Size:        155     565
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     585     720 
   Size:        585     135
 
BsaAI yAC'GTr

Cuts at:      0     560     720 
   Size:        560     160
 
BsaBI GATnn'nnATC

Cuts at:      0     127     720 
   Size:        127     593
 
BsaWI w'CCGG_w

Cuts at:      0     367     720 
   Size:        367     353
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     153     183     565     595     720 
   Size:        153      30     382      30     125

  Fragments arranged by size: 

                 382     153     125      30      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     268     720 
   Size:        268     452
 
BsiEI CG_ry'CG

Cuts at:      0       8     720 
   Size:          8     712
 
BsiHKAI G_wGCw'C

Cuts at:      0      26     515     720 
   Size:         26     489     205

  Fragments arranged by size: 

                 489     205      26
 
Bsp1286I G_dGCh'C

Cuts at:      0      26     515     720 
   Size:         26     489     205

  Fragments arranged by size: 

                 489     205      26
 
BspEI T'CCGG_A

Cuts at:      0     367     720 
   Size:        367     353
 
BssHII G'CGCG_C

Cuts at:      0     313     720 
   Size:        313     407
 
BstBI TT'CG_AA

Cuts at:      0     291     720 
   Size:        291     429
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      18     629     720 
   Size:         18     611      91

  Fragments arranged by size: 

                 611      91      18
 
BstYI r'GATC_y

Cuts at:      0     200     327     720 
   Size:        200     127     393

  Fragments arranged by size: 

                 393     200     127
 
BtgI C'CryG_G

Cuts at:      0     152     539     688     720 
   Size:        152     387     149      32

  Fragments arranged by size: 

                 387     152     149      32
 
BtsI GCAGTG_nn'

Cuts at:      0     357     720 
   Size:        357     363
 
DraIII CAC_nnn'GTG

Cuts at:      0     694     720 
   Size:        694      26
 
EaeI y'GGCC_r

Cuts at:      0     254     720 
   Size:        254     466
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     155     352     720 
   Size:         46     109     197     368

  Fragments arranged by size: 

                 368     197     109      46
 
EcoO109I rG'GnC_Cy

Cuts at:      0     416     720 
   Size:        416     304
 
HaeII r_GCGC'y

Cuts at:      0     192     720 
   Size:        192     528
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     153     185     720 
   Size:        153      32     535

  Fragments arranged by size: 

                 535     153      32
 
MfeI C'AATT_G

Cuts at:      0     623     720 
   Size:        623      97
 
MscI TGG'CCA

Cuts at:      0     256     720 
   Size:        256     464
 
MslI CAynn'nnrTG

Cuts at:      0     538     720 
   Size:        538     182
 
MspA1I CmG'CkG

Cuts at:      0      78     252     720 
   Size:         78     174     468

  Fragments arranged by size: 

                 468     174      78
 
NspI r_CATG'y

Cuts at:      0     537     720 
   Size:        537     183
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     183     720 
   Size:        183     537
 
PfoI T'CCnGG_A

Cuts at:      0     698     720 
   Size:        698      22
 
PpuMI rG'GwC_Cy

Cuts at:      0     416     720 
   Size:        416     304
 
PstI C_TGCA'G

Cuts at:      0     141     197     720 
   Size:        141      56     523

  Fragments arranged by size: 

                 523     141      56
 
PvuII CAG'CTG

Cuts at:      0      78     252     720 
   Size:         78     174     468

  Fragments arranged by size: 

                 468     174      78
 
SanDI GG'GwC_CC

Cuts at:      0     416     720 
   Size:        416     304
 
SexAI A'CCwGG_T

Cuts at:      0     575     720 
   Size:        575     145
 
SfcI C'TryA_G

Cuts at:      0     137     193     213     720 
   Size:        137      56      20     507

  Fragments arranged by size: 

                 507     137      56      20
 
SmaI CCC'GGG

Cuts at:      0     421     720 
   Size:        421     299
 
TatI w'GTAC_w

Cuts at:      0      14     143     720 
   Size:         14     129     577

  Fragments arranged by size: 

                 577     129      14
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     565     597     680     712     720 
   Size:        565      32      83      32       8

  Fragments arranged by size: 

                 565      83      32      32       8
 
Tth111I GACn'n_nGTC

Cuts at:      0     165     720 
   Size:        165     555
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     583     720 
   Size:        583     137
 
XmaI C'CCGG_G

Cuts at:      0     419     720 
   Size:        419     301
 

 Enzymes that do cut:

     AcuI    ApaLI     AvaI     BbsI    BglII Bme1580I     BplI     BpmI
     BsaI    BsaAI    BsaBI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI
 Bsp1286I    BspEI   BssHII    BstBI    BstXI    BstYI     BtgI     BtsI
   DraIII     EaeI  Eco57MI EcoO109I    HaeII    Hin4I     MfeI     MscI
     MslI   MspA1I     NspI    PflMI     PfoI    PpuMI     PstI    PvuII
    SanDI    SexAI     SfcI     SmaI     TatI     TstI  Tth111I     XcmI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
    BmgBI     BmrI     BmtI   Bpu10I    BpuEI    BsaHI    BseRI    BseYI
    BsiWI     BsmI    BsmBI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI    BssSI   BstAPI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     KpnI     MluI     MmeI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI     SapI     SbfI
     ScaI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     XbaI
     XhoI     XmnI     ZraI
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