Restriction map for pF1KB7929
 (Linear) (Six-base) MAPSORT of: KIBB7929.seq  Check: 5956  from: 1  to: 1056



KIBB7929.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     336    1056 
   Size:        336     720
 
AatII G_ACGT'C

Cuts at:      0     378    1056 
   Size:        378     678
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     518     833    1056 
   Size:        518     315     223

  Fragments arranged by size: 

                 518     315     223
 
AflII C'TTAA_G

Cuts at:      0     382    1056 
   Size:        382     674
 
AflIII A'CryG_T

Cuts at:      0     696     905    1056 
   Size:        696     209     151

  Fragments arranged by size: 

                 696     209     151
 
AlwNI CAG_nnn'CTG

Cuts at:      0     231     811    1056 
   Size:        231     580     245

  Fragments arranged by size: 

                 580     245     231
 
ApoI r'AATT_y

Cuts at:      0     582     772    1056 
   Size:        582     190     284

  Fragments arranged by size: 

                 582     284     190
 
AvaI C'yCGr_G

Cuts at:      0     121     750    1056 
   Size:        121     629     306

  Fragments arranged by size: 

                 629     306     121
 
BamHI G'GATC_C

Cuts at:      0     853    1056 
   Size:        853     203
 
BanI G'GyrC_C

Cuts at:      0      55     187     639    1056 
   Size:         55     132     452     417

  Fragments arranged by size: 

                 452     417     132      55
 
BbeI G_GCGC'C

Cuts at:      0      59     191    1056 
   Size:         59     132     865

  Fragments arranged by size: 

                 865     132      59
 
BbsI GAAGACnn'nnnn_

Cuts at:      0       6    1056 
   Size:          6    1050
 
BlpI GC'TnA_GC

Cuts at:      0      25     469    1056 
   Size:         25     444     587

  Fragments arranged by size: 

                 587     444      25
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     547    1056 
   Size:        547     509
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     136     238    1056 
   Size:        136     102     818

  Fragments arranged by size: 

                 818     136     102
 
BsaAI yAC'GTr

Cuts at:      0     636     908    1056 
   Size:        636     272     148

  Fragments arranged by size: 

                 636     272     148
 
BsaHI Gr'CG_yC

Cuts at:      0      56     188     375    1056 
   Size:         56     132     187     681

  Fragments arranged by size: 

                 681     187     132      56
 
BsaWI w'CCGG_w

Cuts at:      0     176    1056 
   Size:        176     880
 
BseYI C'CCAG_C

Cuts at:      0     413    1056 
   Size:        413     643
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     364    1056 
   Size:        364     692
 
BsmI GAATG_Cn'

Cuts at:      0     131    1056 
   Size:        131     925
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     336    1056 
   Size:        336     720
 
BsrBI CCG'CTC

Cuts at:      0      66      72    1056 
   Size:         66       6     984

  Fragments arranged by size: 

                 984      66       6
 
BsrDI GCAATG_nn'

Cuts at:      0     462    1056 
   Size:        462     594
 
BsrFI r'CCGG_y

Cuts at:      0      80     184    1056 
   Size:         80     104     872

  Fragments arranged by size: 

                 872     104      80
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     231    1056 
   Size:        231     825
 
BstYI r'GATC_y

Cuts at:      0     754     853    1056 
   Size:        754      99     203

  Fragments arranged by size: 

                 754     203      99
 
BtsI GCAGTG_nn'

Cuts at:      0     265     486    1056 
   Size:        265     221     570

  Fragments arranged by size: 

                 570     265     221
 
EaeI y'GGCC_r

Cuts at:      0      78     704    1056 
   Size:         78     626     352

  Fragments arranged by size: 

                 626     352      78
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     518     833    1056 
   Size:        518     315     223

  Fragments arranged by size: 

                 518     315     223
 
EcoO109I rG'GnC_Cy

Cuts at:      0     228    1056 
   Size:        228     828
 
EcoRI G'AATT_C

Cuts at:      0     582    1056 
   Size:        582     474
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     330     434     678    1056 
   Size:        330     104     244     378

  Fragments arranged by size: 

                 378     330     244     104
 
FseI GG_CCGG'CC

Cuts at:      0      84    1056 
   Size:         84     972
 
HaeII r_GCGC'y

Cuts at:      0      59     191    1056 
   Size:         59     132     865

  Fragments arranged by size: 

                 865     132      59
 
KasI G'GCGC_C

Cuts at:      0      55     187    1056 
   Size:         55     132     869

  Fragments arranged by size: 

                 869     132      55
 
MscI TGG'CCA

Cuts at:      0     706    1056 
   Size:        706     350
 
MslI CAynn'nnrTG

Cuts at:      0     718    1056 
   Size:        718     338
 
MspA1I CmG'CkG

Cuts at:      0      29      92     986    1041    1056 
   Size:         29      63     894      55      15

  Fragments arranged by size: 

                 894      63      55      29      15
 
NaeI GCC'GGC

Cuts at:      0      82    1056 
   Size:         82     974
 
NarI GG'CG_CC

Cuts at:      0      56     188    1056 
   Size:         56     132     868

  Fragments arranged by size: 

                 868     132      56
 
NgoMIV G'CCGG_C

Cuts at:      0      80    1056 
   Size:         80     976
 
NspI r_CATG'y

Cuts at:      0     700    1056 
   Size:        700     356
 
PciI A'CATG_T

Cuts at:      0     696    1056 
   Size:        696     360
 
PfoI T'CCnGG_A

Cuts at:      0      98    1056 
   Size:         98     958
 
PstI C_TGCA'G

Cuts at:      0     974    1056 
   Size:        974      82
 
PvuII CAG'CTG

Cuts at:      0     986    1056 
   Size:        986      70
 
SfcI C'TryA_G

Cuts at:      0      87     970    1056 
   Size:         87     883      86

  Fragments arranged by size: 

                 883      87      86
 
SfoI GGC'GCC

Cuts at:      0      57     189    1056 
   Size:         57     132     867

  Fragments arranged by size: 

                 867     132      57
 
SmlI C'TyrA_G

Cuts at:      0     151     253     382    1056 
   Size:        151     102     129     674

  Fragments arranged by size: 

                 674     151     129     102
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     316     576    1056 
   Size:        316     260     480

  Fragments arranged by size: 

                 480     316     260
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     410    1056 
   Size:        410     646
 
ZraI GAC'GTC

Cuts at:      0     376    1056 
   Size:        376     680
 

 Enzymes that do cut:

     AarI    AatII     AcuI    AflII   AflIII    AlwNI     ApoI     AvaI
    BamHI     BanI     BbeI     BbsI     BlpI     BmrI    BpuEI    BsaAI
    BsaHI    BsaWI    BseYI     BsgI     BsmI    BspMI    BsrBI    BsrDI
    BsrFI   BstAPI    BstYI     BtsI     EaeI  Eco57MI EcoO109I    EcoRI
     FalI     FseI    HaeII     KasI     MscI     MslI   MspA1I     NaeI
     NarI   NgoMIV     NspI     PciI     PfoI     PstI    PvuII     SfcI
     SfoI     SmlI    TaqII     XcmI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AfeI     AgeI     AhdI     AleI     AlfI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BanII    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
 Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaBI
    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI
    BspHI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRV     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com