Restriction map for pF1KB6285
##################################
# NO CUT ENZYMES (   97 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaLI       AscI        Asp718I     AspEI       
AviII       BalI        BamHI       BbrPI       BcgI        
BciVI       BclI        BglI        BglII       BsaAI       
BsaBI       BseAI       BsiWI       BsmI        BspDI       
BspEI       BspHI       BspLU11I    BspMI       BssHII      
Bst1107I    BstAPI      BstBI       BstUI       BstXI       
BstZ17I     ClaI        CpoI        DraI        DraIII      
DrdI        Eam1105I    Eco47III    EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HindIII     
HpaI        KpnI        KspI        MaeII       MamI        
MluI        MluNI       MroI        MscI        MvnI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PinAI       PmaCI       PmeI        
PmlI        Psp1406I    PstI        PvuI        RcaI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SfiI        SfuI        SmaI        SnaBI       
SphI        Sse8387I    SspI        SwaI        XcmI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AocI         2508  CCTCAGG              CC^T[ACGT]AGG
Asp700I      1954  GAACCATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1051  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII         256  CCTAGG               C^CTAGG
BlnI          256  CCTAGG               C^CTAGG
BlpI         2107  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2107  GCTCAGC              GC^T[ACGT]AGC
BsgI         1427  GTGCAG               GTGCAG(16/14)
BsiEI          80  CGGCCG               CG[GA][CT]^CG
BsmBI        2351  GAGACG               CGTCTC(1/5)
Bsp1407I      478  TGTACA               T^GTACA
BsrBI          32  CCGCTC               CCGCTC(-3/-3)
BsrGI         478  TGTACA               T^GTACA
BssSI        2496  CACGAG               CACGAG(-5/-1)
BstYI        2277  AGATCC               [GA]^GATC[CT]
Bsu36I       2508  CCTCAGG              CC^T[ACGT]AGG
CelII        2107  GCTCAGC              GC^T[ACGT]AGC
EaeI           80  CGGCCG               [CT]^GGCC[GA]
EagI           80  CGGCCG               C^GGCCG
EarI         2394  GAAGAG               CTCTTC(1/4)
EclXI          80  CGGCCG               C^GGCCG
Eco52I         80  CGGCCG               C^GGCCG
Eco81I       2508  CCTCAGG              CC^T[ACGT]AGG
EcoNI        1603  CCTTCAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII       1873  GTCAAC               GT[CT]^[GA]AC
HindII       1873  GTCAAC               GT[CT]^[GA]AC
Ksp632I      2394  GAAGAG               CTCTTC(1/4)
MflI         2277  AGATCC               [GA]^GATC[CT]
MslI          111  CACTCCGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI           71  CCATGG               C^CATGG
NspI          817  GCATGT               [GA]CATG^[CT]
PaeR7I       2558  CTCGAG               C^TCGAG
PflFI        1051  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1192  CCACTTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         199  GGGACCC              [GA]G^G[AT]CC[CT]
PshAI        1335  GACCACGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfcI         1106  CTACAG               C^T[GA][CT]AG
SgrAI          24  CGCCGGCG             C[GA]^CCGG[CT]G
SspBI         478  TGTACA               T^GTACA
StuI         2079  AGGCCT               AGG^CCT
Tth111I      1051  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1192  CCACTTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         2085  TCTAGA               T^CTAGA
XhoI         2558  CTCGAG               C^TCGAG
XhoII        2277  AGATCC               [GA]^GATC[CT]
XmnI         1954  GAACCATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AflII         967  CTTAAG               C^TTAAG
             2245  CTTAAG              
ApaI         1245  GGGCCC               GGGCC^C
             1533  GGGCCC              
AseI          504  ATTAAT               AT^TAAT
              745  ATTAAT              
AsnI          504  ATTAAT               AT^TAAT
              745  ATTAAT              
AvaI           57  CTCGGG               C^[CT]CG[GA]G
             2558  CTCGAG              
BanI           28  GGCGCC               G^G[CT][GA]CC
              127  GGCGCC              
BbeI           28  GGCGCC               GGCGC^C
              127  GGCGCC              
BbvCI         391  CCTCAGC              CCTCAGC(-5/-2)
              943  CCTCAGC             
BfrI          967  CTTAAG               C^TTAAG
             2245  CTTAAG              
BsaWI         114  TCCGGT               [AT]^CCGG[AT]
              222  TCCGGT              
BseRI         269  CTCCTC               GAGGAG(10/8)
              389  CTCCTC              
BsoBI          57  CTCGGG               C^[CT]CG[GA]G
             2558  CTCGAG              
BstEII        207  GGTGACC              G^GT[ACGT]ACC
              564  GGTAACC             
BstPI         207  GGTGACC              G^GT[ACGT]ACC
              564  GGTAACC             
DraII         199  GGGACCC              [GA]G^G[ACGT]CC[CT]
             1533  GGGCCCT             
EcoO109I      199  GGGACCC              [GA]G^G[ACGT]CC[CT]
             1533  GGGCCCT             
EcoO65I       207  GGTGACC              G^GT[ACGT]ACC
              564  GGTAACC             
KasI           28  GGCGCC               G^GCGCC
              127  GGCGCC              
NaeI            7  GCCGGC               GCC^GGC
               25  GCCGGC              
NarI           28  GGCGCC               GG^CGCC
              127  GGCGCC              
NgoMIV          7  GCCGGC               G^CCGGC
               25  GCCGGC              
PleI         1503  GACTC                GAGTC(4/5)
             2259  GAGTC               
PshBI         504  ATTAAT               AT^TAAT
              745  ATTAAT              
SacI         1973  GAGCTC               GAGCT^C
             2561  GAGCTC              
SexAI         953  ACCTGGT              A^CC[AT]GGT
             2164  ACCAGGT             
SfoI           28  GGCGCC               GGC^GCC
              127  GGCGCC              
SpeI         1587  ACTAGT               A^CTAGT
             1708  ACTAGT              


#########################################
# 3 OR MORE CUT ENZYMES (   88 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApoI        AspHI       AvaII       
BanII       BbsI        BbvI        BcnI        BfaI        
BmyI        BpmI        BpuAI       BsaHI       BsaI        
BsaJI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrDI       BsrFI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DsaI        
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        MaeI        MaeIII      MboI        MboII       
MfeI        MnlI        MseI        MspA1I      MspI        
MunI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     
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