Restriction map for pF1KB6285
 (Linear) (Six-base) MAPSORT of: KIBB6285.seq  Check: 5747  from: 1  to: 2586



KIBB6285.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:20  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     685    1276    1406    1589    2586 
   Size:        685     591     130     183     997

  Fragments arranged by size: 

                 997     685     591     183     130
 
AflII C'TTAA_G

Cuts at:      0     967    2245    2586 
   Size:        967    1278     341

  Fragments arranged by size: 

                1278     967     341
 
AleI CACnn'nnGTG

Cuts at:      0     115    2586 
   Size:        115    2471
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2035    2067    2586 
   Size:       2035      32     519

  Fragments arranged by size: 

                2035     519      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     155     183     736    2586 
   Size:        155      28     553    1850

  Fragments arranged by size: 

                1850     553     155      28
 
ApaI G_GGCC'C

Cuts at:      0    1249    1537    2586 
   Size:       1249     288    1049

  Fragments arranged by size: 

                1249    1049     288
 
ApoI r'AATT_y

Cuts at:      0     904    1763    1988    2179    2586 
   Size:        904     859     225     191     407

  Fragments arranged by size: 

                 904     859     407     225     191
 
AseI AT'TA_AT

Cuts at:      0     505     746    2586 
   Size:        505     241    1840

  Fragments arranged by size: 

                1840     505     241
 
AvaI C'yCGr_G

Cuts at:      0      57    2558    2586 
   Size:         57    2501      28

  Fragments arranged by size: 

                2501      57      28
 
AvrII C'CTAG_G

Cuts at:      0     256    2586 
   Size:        256    2330
 
BanI G'GyrC_C

Cuts at:      0      28     127    2586 
   Size:         28      99    2459

  Fragments arranged by size: 

                2459      99      28
 
BanII G_rGCy'C

Cuts at:      0     167     710    1249    1537    1977    2565    2586 
   Size:        167     543     539     288     440     588      21

  Fragments arranged by size: 

                 588     543     539     440     288     167      21
 
BbeI G_GCGC'C

Cuts at:      0      32     131    2586 
   Size:         32      99    2455

  Fragments arranged by size: 

                2455      99      32
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     929    1432    1684    2586 
   Size:        929     503     252     902

  Fragments arranged by size: 

                 929     902     503     252
 
BbvCI CC'TCA_GC

Cuts at:      0     392     944    2586 
   Size:        392     552    1642

  Fragments arranged by size: 

                1642     552     392
 
BlpI GC'TnA_GC

Cuts at:      0    2108    2586 
   Size:       2108     478
 
Bme1580I G_kGCm'C

Cuts at:      0    1249    1537    2586 
   Size:       1249     288    1049

  Fragments arranged by size: 

                1249    1049     288
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     323    1169    1241    1490    1706    2586 
   Size:        323     846      72     249     216     880

  Fragments arranged by size: 

                 880     846     323     249     216      72
 
Bpu10I CC'TnA_GC

Cuts at:      0     392     944    2586 
   Size:        392     552    1642

  Fragments arranged by size: 

                1642     552     392
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     420     916    1101    2586 
   Size:        420     496     185    1485

  Fragments arranged by size: 

                1485     496     420     185
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     409    1347    2391    2586 
   Size:        409     938    1044     195

  Fragments arranged by size: 

                1044     938     409     195
 
BsaHI Gr'CG_yC

Cuts at:      0      29     128     172    2586 
   Size:         29      99      44    2414

  Fragments arranged by size: 

                2414      99      44      29
 
BsaWI w'CCGG_w

Cuts at:      0     114     222    2586 
   Size:        114     108    2364

  Fragments arranged by size: 

                2364     114     108
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     126     156     615     645    1074    1104    2035    2065 
   Size:        126      30     459      30     429      30     931      30

Cuts at:   2065    2536    2566    2586 
   Size:        471      30      20

  Fragments arranged by size: 

                 931     471     459     429     126      30      30      30
                  30      30      20
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     260     380    2586 
   Size:        260     120    2206

  Fragments arranged by size: 

                2206     260     120
 
BseYI C'CCAG_C

Cuts at:      0     176    1471    1526    1555    2586 
   Size:        176    1295      55      29    1031

  Fragments arranged by size: 

                1295    1031     176      55      29
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1448    2586 
   Size:       1448    1138
 
BsiEI CG_ry'CG

Cuts at:      0      83    2586 
   Size:         83    2503
 
BsiHKAI G_wGCw'C

Cuts at:      0     112    1977    2565    2586 
   Size:        112    1865     588      21

  Fragments arranged by size: 

                1865     588     112      21
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2345    2586 
   Size:       2345     241
 
Bsp1286I G_dGCh'C

Cuts at:      0     112     167     710    1249    1537    1977    2565    2586 
   Size:        112      55     543     539     288     440     588      21

  Fragments arranged by size: 

                 588     543     539     440     288     112      55      21
            
 
BsrBI CCG'CTC

Cuts at:      0      34    2586 
   Size:         34    2552
 
BsrDI GCAATG_nn'

Cuts at:      0     806    1033    1058    1620    2586 
   Size:        806     227      25     562     966

  Fragments arranged by size: 

                 966     806     562     227      25
 
BsrFI r'CCGG_y

Cuts at:      0       7      25      93    2586 
   Size:          7      18      68    2493

  Fragments arranged by size: 

                2493      68      18       7
 
BsrGI T'GTAC_A

Cuts at:      0     478    2586 
   Size:        478    2108
 
BssSI C'ACGA_G

Cuts at:      0    2496    2586 
   Size:       2496      90
 
BstEII G'GTnAC_C

Cuts at:      0     207     564    2586 
   Size:        207     357    2022

  Fragments arranged by size: 

                2022     357     207
 
BstYI r'GATC_y

Cuts at:      0    2277    2586 
   Size:       2277     309
 
Bsu36I CC'TnA_GG

Cuts at:      0    2509    2586 
   Size:       2509      77
 
BtgI C'CryG_G

Cuts at:      0      71     167     194    1337    2586 
   Size:         71      96      27    1143    1249

  Fragments arranged by size: 

                1249    1143      96      71      27
 
BtsI GCAGTG_nn'

Cuts at:      0     527    1345    2586 
   Size:        527     818    1241

  Fragments arranged by size: 

                1241     818     527
 
EaeI y'GGCC_r

Cuts at:      0      80    2586 
   Size:         80    2506
 
EagI C'GGCC_G

Cuts at:      0      80    2586 
   Size:         80    2506
 
EarI CTCTTCn'nnn_

Cuts at:      0    2389    2586 
   Size:       2389     197
 
EcoICRI GAG'CTC

Cuts at:      0    1975    2563    2586 
   Size:       1975     588      23

  Fragments arranged by size: 

                1975     588      23
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     323     685    1169    1241    1276    1406    1490    1589 
   Size:        323     362     484      72      35     130      84      99

Cuts at:   1589    1706    2586 
   Size:        117     880

  Fragments arranged by size: 

                 880     484     362     323     130     117      99      84
                  72      35
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1607    2586 
   Size:       1607     979
 
EcoO109I rG'GnC_Cy

Cuts at:      0     200    1534    2586 
   Size:        200    1334    1052

  Fragments arranged by size: 

                1334    1052     200
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2452    2586 
   Size:       2452     134
 
HaeII r_GCGC'y

Cuts at:      0      32      56      91     131     233    2586 
   Size:         32      24      35      40     102    2353

  Fragments arranged by size: 

                2353     102      40      35      32      24
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     126     158    2152    2184    2586 
   Size:        126      32    1994      32     402

  Fragments arranged by size: 

                1994     402     126      32      32
 
HincII GTy'rAC

Cuts at:      0    1875    2586 
   Size:       1875     711
 
KasI G'GCGC_C

Cuts at:      0      28     127    2586 
   Size:         28      99    2459

  Fragments arranged by size: 

                2459      99      28
 
MfeI C'AATT_G

Cuts at:      0    2102    2200    2323    2586 
   Size:       2102      98     123     263

  Fragments arranged by size: 

                2102     263     123      98
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     781    2586 
   Size:        781    1805
 
MslI CAynn'nnrTG

Cuts at:      0     115    2586 
   Size:        115    2471
 
MspA1I CmG'CkG

Cuts at:      0     251     736    1276    1555    2289    2586 
   Size:        251     485     540     279     734     297

  Fragments arranged by size: 

                 734     540     485     297     279     251
 
NaeI GCC'GGC

Cuts at:      0       9      27    2586 
   Size:          9      18    2559

  Fragments arranged by size: 

                2559      18       9
 
NarI GG'CG_CC

Cuts at:      0      29     128    2586 
   Size:         29      99    2458

  Fragments arranged by size: 

                2458      99      29
 
NcoI C'CATG_G

Cuts at:      0      71    2586 
   Size:         71    2515
 
NgoMIV G'CCGG_C

Cuts at:      0       7      25    2586 
   Size:          7      18    2561

  Fragments arranged by size: 

                2561      18       7
 
NspI r_CATG'y

Cuts at:      0     821    2586 
   Size:        821    1765
 
PasI CC'CwG_GG

Cuts at:      0     123    2586 
   Size:        123    2463
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1198    2586 
   Size:       1198    1388
 
PfoI T'CCnGG_A

Cuts at:      0     339     633    2586 
   Size:        339     294    1953

  Fragments arranged by size: 

                1953     339     294
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     481     513    2035    2067    2586 
   Size:        481      32    1522      32     519

  Fragments arranged by size: 

                1522     519     481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     200    2586 
   Size:        200    2386
 
PshAI GACnn'nnGTC

Cuts at:      0    1339    2586 
   Size:       1339    1247
 
PspOMI G'GGCC_C

Cuts at:      0    1245    1533    2586 
   Size:       1245     288    1053

  Fragments arranged by size: 

                1245    1053     288
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     557     589    2586 
   Size:        557      32    1997

  Fragments arranged by size: 

                1997     557      32
 
PvuII CAG'CTG

Cuts at:      0     736    1276    1555    2289    2586 
   Size:        736     540     279     734     297

  Fragments arranged by size: 

                 736     734     540     297     279
 
SacI G_AGCT'C

Cuts at:      0    1977    2565    2586 
   Size:       1977     588      21

  Fragments arranged by size: 

                1977     588      21
 
SanDI GG'GwC_CC

Cuts at:      0     200    2586 
   Size:        200    2386
 
SexAI A'CCwGG_T

Cuts at:      0     953    2164    2586 
   Size:        953    1211     422

  Fragments arranged by size: 

                1211     953     422
 
SfcI C'TryA_G

Cuts at:      0    1106    2586 
   Size:       1106    1480
 
SfoI GGC'GCC

Cuts at:      0      30     129    2586 
   Size:         30      99    2457

  Fragments arranged by size: 

                2457      99      30
 
SgrAI Cr'CCGG_yG

Cuts at:      0      25    2586 
   Size:         25    2561
 
SmlI C'TyrA_G

Cuts at:      0     435     931     967    1116    2245    2558    2586 
   Size:        435     496      36     149    1129     313      28

  Fragments arranged by size: 

                1129     496     435     313     149      36      28
 
SpeI A'CTAG_T

Cuts at:      0    1587    1708    2586 
   Size:       1587     121     878

  Fragments arranged by size: 

                1587     878     121
 
StuI AGG'CCT

Cuts at:      0    2081    2586 
   Size:       2081     505
 
StyI C'CwwG_G

Cuts at:      0      71     256    2386    2586 
   Size:         71     185    2130     200

  Fragments arranged by size: 

                2130     200     185      71
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1176    2586 
   Size:       1176    1410
 
TatI w'GTAC_w

Cuts at:      0     478     591    1069    2586 
   Size:        478     113     478    1517

  Fragments arranged by size: 

                1517     478     478     113
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     545     577     615     647    2586 
   Size:        545      32      38      32    1939

  Fragments arranged by size: 

                1939     545      38      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1054    2586 
   Size:       1054    1532
 
XbaI T'CTAG_A

Cuts at:      0    2085    2586 
   Size:       2085     501
 
XhoI C'TCGA_G

Cuts at:      0    2558    2586 
   Size:       2558      28
 
XmnI GAAnn'nnTTC

Cuts at:      0    1958    2586 
   Size:       1958     628
 

 Enzymes that do cut:

     AcuI    AflII     AleI     AloI    AlwNI     ApaI     ApoI     AseI
     AvaI    AvrII     BanI    BanII     BbeI     BbsI    BbvCI     BlpI
 Bme1580I     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BsrBI
    BsrDI    BsrFI    BsrGI    BssSI   BstEII    BstYI   Bsu36I     BtgI
     BtsI     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I
     FalI    HaeII    Hin4I   HincII     KasI     MfeI     MmeI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI    PflMI
     PfoI     PpiI    PpuMI    PshAI   PspOMI     PsrI    PvuII     SacI
    SanDI    SexAI     SfcI     SfoI    SgrAI     SmlI     SpeI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AlfI    ApaLI     AscI    AsiSI     BaeI    BamHI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII    BmgBI     BmrI     BmtI
     BplI    BsaAI    BsaBI    BsiWI     BsmI    BspEI    BspHI    BspMI
   BssHII   BstAPI    BstBI    BstXI  BstZ17I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EciI    EcoRI    EcoRV     FseI     FspI
    FspAI  HindIII     HpaI     KpnI     MluI     MscI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI     PsiI
    PspXI     PstI     PvuI    RsrII    SacII     SalI     SapI     SbfI
     ScaI     SfiI     SgfI     SmaI    SnaBI     SphI     SrfI     SspI
     SwaI     XcmI     XmaI     ZraI
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