Restriction map for pF1KB6912
 (Linear) (Six-base) MAPSORT of: KIBB6912.seq  Check: 2102  from: 1  to: 654



KIBB6912.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AatII G_ACGT'C

Cuts at:      0     173     654 
   Size:        173     481
 
AflIII A'CryG_T

Cuts at:      0     396     654 
   Size:        396     258
 
AleI CACnn'nnGTG

Cuts at:      0     295     654 
   Size:        295     359
 
BanI G'GyrC_C

Cuts at:      0     284     423     465     643     654 
   Size:        284     139      42     178      11

  Fragments arranged by size: 

                 284     178     139      42      11
 
BanII G_rGCy'C

Cuts at:      0     566     654 
   Size:        566      88
 
BbeI G_GCGC'C

Cuts at:      0     288     469     654 
   Size:        288     181     185

  Fragments arranged by size: 

                 288     185     181
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     373     479     629     654 
   Size:        373     106     150      25

  Fragments arranged by size: 

                 373     150     106      25
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     578     654 
   Size:        578      76
 
Bme1580I G_kGCm'C

Cuts at:      0     428     646     654 
   Size:        428     218       8

  Fragments arranged by size: 

                 428     218       8
 
BmgBI CAC'GTC

Cuts at:      0     399     654 
   Size:        399     255
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     109     654 
   Size:        109     545
 
BsaHI Gr'CG_yC

Cuts at:      0     141     170     285     466     654 
   Size:        141      29     115     181     188

  Fragments arranged by size: 

                 188     181     141     115      29
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     451     454     496     574     654 
   Size:        451       3      42      78      80

  Fragments arranged by size: 

                 451      80      78      42       3
 
BseYI C'CCAG_C

Cuts at:      0      92     654 
   Size:         92     562
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     598     654 
   Size:        598      56
 
BsiEI CG_ry'CG

Cuts at:      0     130     207     654 
   Size:        130      77     447

  Fragments arranged by size: 

                 447     130      77
 
Bsp1286I G_dGCh'C

Cuts at:      0     428     566     646     654 
   Size:        428     138      80       8

  Fragments arranged by size: 

                 428     138      80       8
 
BsrBI CCG'CTC

Cuts at:      0     216     654 
   Size:        216     438
 
BsrFI r'CCGG_y

Cuts at:      0     143     150     462     654 
   Size:        143       7     312     192

  Fragments arranged by size: 

                 312     192     143       7
 
BssHII G'CGCG_C

Cuts at:      0      54     654 
   Size:         54     600
 
BssSI C'ACGA_G

Cuts at:      0     433     654 
   Size:        433     221
 
BtgI C'CryG_G

Cuts at:      0      22     330     654 
   Size:         22     308     324

  Fragments arranged by size: 

                 324     308      22
 
EaeI y'GGCC_r

Cuts at:      0     127     204     654 
   Size:        127      77     450

  Fragments arranged by size: 

                 450     127      77
 
EagI C'GGCC_G

Cuts at:      0     127     204     654 
   Size:        127      77     450

  Fragments arranged by size: 

                 450     127      77
 
EarI CTCTTCn'nnn_

Cuts at:      0     223     654 
   Size:        223     431
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     163     654 
   Size:        163     491
 
HaeII r_GCGC'y

Cuts at:      0     288     469     654 
   Size:        288     181     185

  Fragments arranged by size: 

                 288     185     181
 
KasI G'GCGC_C

Cuts at:      0     284     465     654 
   Size:        284     181     189

  Fragments arranged by size: 

                 284     189     181
 
MslI CAynn'nnrTG

Cuts at:      0     295     654 
   Size:        295     359
 
MspA1I CmG'CkG

Cuts at:      0      24     198     654 
   Size:         24     174     456

  Fragments arranged by size: 

                 456     174      24
 
NaeI GCC'GGC

Cuts at:      0     145     464     654 
   Size:        145     319     190

  Fragments arranged by size: 

                 319     190     145
 
NarI GG'CG_CC

Cuts at:      0     285     466     654 
   Size:        285     181     188

  Fragments arranged by size: 

                 285     188     181
 
NgoMIV G'CCGG_C

Cuts at:      0     143     462     654 
   Size:        143     319     192

  Fragments arranged by size: 

                 319     192     143
 
PasI CC'CwG_GG

Cuts at:      0     599     654 
   Size:        599      55
 
PvuII CAG'CTG

Cuts at:      0     198     654 
   Size:        198     456
 
RsrII CG'GwC_CG

Cuts at:      0     153     654 
   Size:        153     501
 
SacII CC_GC'GG

Cuts at:      0      25     654 
   Size:         25     629
 
SapI GCTCTTCn'nnn_

Cuts at:      0     223     654 
   Size:        223     431
 
SfoI GGC'GCC

Cuts at:      0     286     467     654 
   Size:        286     181     187

  Fragments arranged by size: 

                 286     187     181
 
SgrAI Cr'CCGG_yG

Cuts at:      0     462     654 
   Size:        462     192
 
StuI AGG'CCT

Cuts at:      0     340     445     654 
   Size:        340     105     209

  Fragments arranged by size: 

                 340     209     105
 
StyI C'CwwG_G

Cuts at:      0     638     654 
   Size:        638      16
 
ZraI GAC'GTC

Cuts at:      0     171     654 
   Size:        171     483
 

 Enzymes that do cut:

    AatII   AflIII     AleI     BanI    BanII     BbeI     BbsI    BciVI
 Bme1580I    BmgBI     BsaI    BsaHI    BseRI    BseYI     BsgI    BsiEI
 Bsp1286I    BsrBI    BsrFI   BssHII    BssSI     BtgI     EaeI     EagI
     EarI     EciI    HaeII     KasI     MslI   MspA1I     NaeI     NarI
   NgoMIV     PasI    PvuII    RsrII    SacII     SapI     SfoI    SgrAI
     StuI     StyI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BbvCI     BcgI
     BclI    BfrBI     BglI    BglII     BlpI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaWI    BsaXI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrDI    BsrGI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI
     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI     SacI
     SalI    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI     SgfI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI
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