Restriction map for pF1KB6228
##################################
# NO CUT ENZYMES (  102 enzymes) #
##################################

Acc65I      AccII       AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AviII       
AvrII       BamHI       BbrPI       BbvCI       BcgI        
BclI        BfrI        BlnI        BsaAI       BsaBI       
BseAI       BsiWI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrGI       BssHII      
Bst1107I    BstUI       BstZ17I     ClaI        CpoI        
DraI        DraIII      DrdI        Eam1105I    Eco47III    
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MunI        MvnI        NdeI        
NheI        NotI        NruI        NsiI        NspI        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
ScaI        SexAI       SfiI        SgrAI       SmaI        
SnaBI       SpeI        SphI        SspBI       SspI        
StuI        SwaI        Tth111I     XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AatII         980  GACGTC               GACGT^C
AccI          373  GTCTAC               GT^[AC][GT]AC
Asp700I       192  GAAGCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          341  CTCGGG               C^[CT]CG[GA]G
BbeI         1076  GGCGCC               GGCGC^C
BbsI         1312  GAAGAC               GAAGAC(2/6)
BciVI        1728  GGATAC               GTATCC(6/5)
BglI         2139  GCCTCCTCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI           58  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I       58  GCTCAGC              GC^T[ACGT]AGC
BpuAI        1312  GAAGAC               GAAGAC(2/6)
BsaI         1821  GGTCTC               GGTCTC(1/5)
BsaWI        1091  ACCGGA               [AT]^CCGG[AT]
BsiEI         747  CGGCCG               CG[GA][CT]^CG
BsoBI         341  CTCGGG               C^[CT]CG[GA]G
BsrBI         814  GAGCGG               CCGCTC(-3/-3)
BssSI        2049  CACGAG               CACGAG(-5/-1)
BstAPI        164  GCAAGAAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         199  TTCGAA               TT^CGAA
BstEII       2079  GGTGACC              G^GT[ACGT]ACC
BstPI        2079  GGTGACC              G^GT[ACGT]ACC
BstXI        2048  CCACGAGGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII          58  GCTCAGC              GC^T[ACGT]AGC
EagI          747  CGGCCG               C^GGCCG
EclXI         747  CGGCCG               C^GGCCG
Eco52I        747  CGGCCG               C^GGCCG
EcoO65I      2079  GGTGACC              G^GT[ACGT]ACC
FauI          785  CCCGC                CCCGC(4/6)
HindIII      1715  AAGCTT               A^AGCTT
HpaI          799  GTTAAC               GTT^AAC
KasI         1076  GGCGCC               G^GCGCC
MseI          800  TTAA                 T^TAA
NaeI          353  GCCGGC               GCC^GGC
NarI         1076  GGCGCC               GG^CGCC
NcoI         2070  CCATGG               C^CATGG
NgoMIV        353  GCCGGC               G^CCGGC
NspV          199  TTCGAA               TT^CGAA
SbfI          666  CCTGCAGG             CCTGCA^GG
SfoI         1076  GGCGCC               GGC^GCC
SfuI          199  TTCGAA               TT^CGAA
SmlI          400  CTCAAG               C^T[CT][GA]AG
Sse8387I      666  CCTGCAGG             CCTGCA^GG
Tru9I         800  TTAA                 T^TAA
Tsp509I       641  AATT                 ^AATT
XmnI          192  GAAGCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AfaI         1991  GTAC                 GT^AC
             2153  GTAC                
AocI          244  CCTGAGG              CC^T[ACGT]AGG
             1654  CCTGAGG             
BglII        1502  AGATCT               A^GATCT
             1546  AGATCT              
BsgI          390  CTGCAC               GTGCAG(16/14)
             1049  CTGCAC              
BsmBI        1848  CGTCTC               CGTCTC(1/5)
             1747  GAGACG              
BspMI         665  ACCTGC               ACCTGC(4/8)
             1462  GCAGGT              
BsrDI        1138  GCAATG               GCAATG(2/0)
             1640  GCAATG              
BsrI         1862  ACTGG                ACTGG(1/-1)
             2032  CCAGT               
Bsu36I        244  CCTGAGG              CC^T[ACGT]AGG
             1654  CCTGAGG             
Eco81I        244  CCTGAGG              CC^T[ACGT]AGG
             1654  CCTGAGG             
HaeII         585  GGCGCT               [GA]GCGC^[CT]
             1076  GGCGCC              
HgaI         1574  GACGC                GACGC(5/10)
             1847  GCGTC               
HincII        799  GTTAAC               GT[CT]^[GA]AC
             1042  GTTGAC              
HindII        799  GTTAAC               GT[CT]^[GA]AC
             1042  GTTGAC              
MaeII         279  ACGT                 A^CGT
              981  ACGT                
MslI           18  CATCATCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2049  CACGAGGGTG          
PpuMI         332  GGGTCCT              [GA]G^G[AT]CC[CT]
              538  AGGACCC             
RsaI         1991  GTAC                 GT^AC
             2153  GTAC                
SacI          385  GAGCTC               GAGCT^C
             1184  GAGCTC              
SapI         1181  GAAGAGC              GCTCTTC(1/4)
              734  GCTCTTC             
TaqI          200  TCGA                 T^CGA
              989  TCGA                
TthHB8I       200  TCGA                 T^CGA
              989  TCGA                
XcmI         1103  CCATGAGTCGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2066  CCAGCCATGGCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   88 enzymes) #
#########################################

AciI        AcyI        AluI        AlwI        AlwNI       
ApaI        AspHI       AvaII       BalI        BanI        
BanII       BbvI        BcnI        BfaI        BmyI        
BpmI        BsaHI       BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrFI       BssKI       BstF5I      BstNI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PflMI       PleI        PstI        
PvuII       Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TfiI        TseI        Tsp45I      
TspRI       Van91I      XhoII       
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