Restriction map for pF1KB6217
##################################
# NO CUT ENZYMES (  102 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        AlwI        
Aor51HI     ApoI        AscI        AseI        AsnI        
AspEI       AspI        AviII       AvrII       BamHI       
BbrPI       BbvCI       BcgI        BciVI       BfrI        
BglII       BlnI        BsaAI       BsaBI       BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
BspDI       BspEI       BspLU11I    BsrDI       Bst1107I    
BstBI       BstYI       BstZ17I     ClaI        CpoI        
DraI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoRI       EcoRV       EcoT14I     EcoT22I     
FseI        FspI        HindIII     HpaI        MamI        
MfeI        MflI        MluI        MroI        MunI        
NcoI        NdeI        NheI        NotI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SalI        
SbfI        SfiI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        StyI        SwaI        Tth111I     XbaI        
XhoI        XhoII       


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

Alw44I       1234  GTGCAC               G^TGCAC
AocI          747  CCTGAGG              CC^T[ACGT]AGG
ApaLI        1234  GTGCAC               G^TGCAC
Asp700I      1185  GAATGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BclI         1451  TGATCA               T^GATCA
Bsp1407I      659  TGTACA               T^GTACA
BsrBI         333  CCGCTC               CCGCTC(-3/-3)
BsrFI         691  GCCGGC               [GA]^CCGG[CT]
BsrGI         659  TGTACA               T^GTACA
BssHII       1173  GCGCGC               G^CGCGC
BssSI         685  CTCGTG               CACGAG(-5/-1)
BstAPI        732  GCACCCCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1809  GGTCACC              G^GT[ACGT]ACC
BstPI        1809  GGTCACC              G^GT[ACGT]ACC
Bsu36I        747  CCTGAGG              CC^T[ACGT]AGG
Cfr10I        691  GCCGGC               [GA]^CCGG[CT]
DraIII       1593  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI          349  CTCTTC               CTCTTC(1/4)
Eco81I        747  CCTGAGG              CC^T[ACGT]AGG
EcoNI         984  CCTGGAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1809  GGTCACC              G^GT[ACGT]ACC
FbaI         1451  TGATCA               T^GATCA
HgaI          645  GACGC                GACGC(5/10)
Ksp632I       349  CTCTTC               CTCTTC(1/4)
KspI         1486  CCGCGG               CCGC^GG
MaeII        1499  ACGT                 A^CGT
NaeI          691  GCCGGC               GCC^GGC
NgoMIV        691  GCCGGC               G^CCGGC
PvuII         856  CAGCTG               CAG^CTG
SacII        1486  CCGCGG               CCGC^GG
SapI          348  GCTCTTC              GCTCTTC(1/4)
SmaI         1223  CCCGGG               CCC^GGG
SspBI         659  TGTACA               T^GTACA
TfiI         1145  GAATC                G^A[AT]TC
XmaCI        1223  CCCGGG               C^CCGGG
XmaI         1223  CCCGGG               C^CCGGG
XmnI         1185  GAATGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   37 enzymes) #
###################################

Acc65I        593  GGTACC               G^GTACC
              929  GGTACC              
AcyI          285  GGCGCC               G[GA]^CG[CT]C
              385  GGCGCC              
Asp718I       593  GGTACC               G^GTACC
              929  GGTACC              
AvaI           26  CTCGGG               C^[CT]CG[GA]G
             1223  CCCGGG              
BalI            8  TGGCCA               TGG^CCA
             1739  TGGCCA              
BbeI          285  GGCGCC               GGCGC^C
              385  GGCGCC              
BfaI          149  CTAG                 C^TAG
              209  CTAG                
BglI          430  GCCTTCTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1964  GCCGAGGGGGC         
BlpI           69  GCTCAGC              GC^T[ACGT]AGC
             1885  GCTGAGC             
Bpu1102I       69  GCTCAGC              GC^T[ACGT]AGC
             1885  GCTGAGC             
BsaHI         285  GGCGCC               G[GA]^CG[CT]C
              385  GGCGCC              
BsoBI          26  CTCGGG               C^[CT]CG[GA]G
             1223  CCCGGG              
BspHI         116  TCATGA               T^CATGA
              236  TCATGA              
BstXI         389  CCATCCGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              680  CCACTCTCGTGG        
CelII          69  GCTCAGC              GC^T[ACGT]AGC
             1885  GCTGAGC             
DrdI          544  GACAAGCTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1900  GACCTGCTGGTC        
Hin1I         285  GGCGCC               G[GA]^CG[CT]C
              385  GGCGCC              
KasI          285  GGCGCC               G^GCGCC
              385  GGCGCC              
KpnI          593  GGTACC               GGTAC^C
              929  GGTACC              
MaeI          149  CTAG                 C^TAG
              209  CTAG                
MluNI           8  TGGCCA               TGG^CCA
             1739  TGGCCA              
MscI            8  TGGCCA               TGG^CCA
             1739  TGGCCA              
MseI          605  TTAA                 T^TAA
             1277  TTAA                
MslI          681  CACTCTCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1305  CATCAGCATG          
MspA1I        856  CAGCTG               C[AC]G^C[GT]G
             1486  CCGCGG              
NarI          285  GGCGCC               GG^CGCC
              385  GGCGCC              
NruI          708  TCGCGA               TCG^CGA
             1696  TCGCGA              
PflMI        1118  CCAACCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1494  CCAGAACGTGG         
PpuMI         143  AGGACCC              [GA]G^G[AT]CC[CT]
              752  GGGTCCC             
PstI          157  CTGCAG               CTGCA^G
             2144  CTGCAG              
RcaI          116  TCATGA               T^CATGA
              236  TCATGA              
ScaI          500  AGTACT               AGT^ACT
             1505  AGTACT              
SexAI         168  ACCAGGT              A^CC[AT]GGT
             1031  ACCAGGT             
SfoI          285  GGCGCC               GGC^GCC
              385  GGCGCC              
Tru9I         605  TTAA                 T^TAA
             1277  TTAA                
Van91I       1118  CCAACCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1494  CCAGAACGTGG         
XcmI         1071  CCACCCGATGTATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1629  CCAGCTCTCTGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   82 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwNI       
ApaI        AspHI       AvaII       BanI        BanII       
BbsI        BbvI        BcnI        BmyI        BpmI        
BpuAI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspMI       BsrI        BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        MaeIII      MboI        MboII       MnlI        
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        TaqI        TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     
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