Restriction map for pF1KB6203
 (Linear) (Six-base) MAPSORT of: KIBB6203.seq  Check: 5919  from: 1  to: 1989



KIBB6203.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1353    1989 
   Size:       1353     636
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     102     979    1147    1678    1989 
   Size:        102     877     168     531     311

  Fragments arranged by size: 

                 877     531     311     168     102
 
AflIII A'CryG_T

Cuts at:      0     536     818     926    1146    1989 
   Size:        536     282     108     220     843

  Fragments arranged by size: 

                 843     536     282     220     108
 
AhdI GACnn_n'nnGTC

Cuts at:      0     111    1398    1989 
   Size:        111    1287     591

  Fragments arranged by size: 

                1287     591     111
 
AleI CACnn'nnGTG

Cuts at:      0     334    1942    1989 
   Size:        334    1608      47

  Fragments arranged by size: 

                1608     334      47
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1960    1989 
   Size:       1960      29
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1338    1487    1989 
   Size:       1338     149     502

  Fragments arranged by size: 

                1338     502     149
 
ApaI G_GGCC'C

Cuts at:      0     427    1187    1811    1989 
   Size:        427     760     624     178

  Fragments arranged by size: 

                 760     624     427     178
 
ApaLI G'TGCA_C

Cuts at:      0    1878    1927    1989 
   Size:       1878      49      62

  Fragments arranged by size: 

                1878      62      49
 
AvaI C'yCGr_G

Cuts at:      0     783     838    1178    1989 
   Size:        783      55     340     811

  Fragments arranged by size: 

                 811     783     340      55
 
BanI G'GyrC_C

Cuts at:      0      64     439     879    1936    1983    1989 
   Size:         64     375     440    1057      47       6

  Fragments arranged by size: 

                1057     440     375      64      47       6
 
BanII G_rGCy'C

Cuts at:      0     427     791     836    1187    1240    1811    1989 
   Size:        427     364      45     351      53     571     178

  Fragments arranged by size: 

                 571     427     364     351     178      53      45
 
BbeI G_GCGC'C

Cuts at:      0     883    1989 
   Size:        883    1106
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     530     568     861    1092    1989 
   Size:        530      38     293     231     897

  Fragments arranged by size: 

                 897     530     293     231      38
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     466     500    1989 
   Size:        466      34    1489

  Fragments arranged by size: 

                1489     466      34
 
BfrBI ATG'CAT

Cuts at:      0    1634    1989 
   Size:       1634     355
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1806    1989 
   Size:       1806     183
 
Bme1580I G_kGCm'C

Cuts at:      0      67     427    1187    1359    1811    1882    1931    1988 
   Size:         67     360     760     172     452      71      49      57

Cuts at:   1988    1989 
   Size:          1

  Fragments arranged by size: 

                 760     452     360     172      71      67      57      49
                   1
 
BmgBI CAC'GTC

Cuts at:      0     113     821    1989 
   Size:        113     708    1168

  Fragments arranged by size: 

                1168     708     113
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1237    1876    1989 
   Size:       1237     639     113

  Fragments arranged by size: 

                1237     639     113
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     490    1989 
   Size:        490    1499
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     152     850    1855    1858    1879    1989 
   Size:        152     698    1005       3      21     110

  Fragments arranged by size: 

                1005     698     152     110      21       3
 
Bpu10I CC'TnA_GC

Cuts at:      0     553    1426    1989 
   Size:        553     873     563

  Fragments arranged by size: 

                 873     563     553
 
BsaAI yAC'GTr

Cuts at:      0     606     750    1413    1989 
   Size:        606     144     663     576

  Fragments arranged by size: 

                 663     606     576     144
 
BsaHI Gr'CG_yC

Cuts at:      0     880    1989 
   Size:        880    1109
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     584     614    1831    1856    1861    1886    1989 
   Size:        584      30    1217      25       5      25     103

  Fragments arranged by size: 

                1217     584     103      30      25      25       5
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1030    1033    1852    1989 
   Size:       1030       3     819     137

  Fragments arranged by size: 

                1030     819     137       3
 
BseYI C'CCAG_C

Cuts at:      0      60     905    1272    1306    1358    1668    1989 
   Size:         60     845     367      34      52     310     321

  Fragments arranged by size: 

                 845     367     321     310      60      52      34
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      93     693     951    1387    1989 
   Size:         93     600     258     436     602

  Fragments arranged by size: 

                 602     600     436     258      93
 
BsiEI CG_ry'CG

Cuts at:      0    1824    1989 
   Size:       1824     165
 
BsiHKAI G_wGCw'C

Cuts at:      0     559     602     836    1882    1931    1989 
   Size:        559      43     234    1046      49      58

  Fragments arranged by size: 

                1046     559     234      58      49      43
 
Bsp1286I G_dGCh'C

Cuts at:      0      67     427     559     602     791     836    1187    1240 
   Size:         67     360     132      43     189      45     351      53

Cuts at:   1240    1359    1811    1882    1931    1988    1989 
   Size:        119     452      71      49      57       1

  Fragments arranged by size: 

                 452     360     351     189     132     119      71      67
                  57      53      49      45      43       1
 
BspHI T'CATG_A

Cuts at:      0     890    1989 
   Size:        890    1099
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     536    1353    1989 
   Size:        536     817     636

  Fragments arranged by size: 

                 817     636     536
 
BsrDI GCAATG_nn'

Cuts at:      0    1317    1989 
   Size:       1317     672
 
BsrFI r'CCGG_y

Cuts at:      0     370    1319    1989 
   Size:        370     949     670

  Fragments arranged by size: 

                 949     670     370
 
BstBI TT'CG_AA

Cuts at:      0    1544    1989 
   Size:       1544     445
 
BstEII G'GTnAC_C

Cuts at:      0     225    1989 
   Size:        225    1764
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     450    1989 
   Size:        450    1539
 
Bsu36I CC'TnA_GG

Cuts at:      0     101    1013    1989 
   Size:        101     912     976

  Fragments arranged by size: 

                 976     912     101
 
BtgI C'CryG_G

Cuts at:      0     332     488    1094    1403    1524    1989 
   Size:        332     156     606     309     121     465

  Fragments arranged by size: 

                 606     465     332     309     156     121
 
BtsI GCAGTG_nn'

Cuts at:      0    1929    1989 
   Size:       1929      60
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     865    1989 
   Size:        865    1124
 
EaeI y'GGCC_r

Cuts at:      0     245     608     977    1472    1676    1821    1989 
   Size:        245     363     369     495     204     145     168

  Fragments arranged by size: 

                 495     369     363     245     204     168     145
 
EagI C'GGCC_G

Cuts at:      0    1821    1989 
   Size:       1821     168
 
EarI CTCTTCn'nnn_

Cuts at:      0    1291    1714    1989 
   Size:       1291     423     275

  Fragments arranged by size: 

                1291     423     275
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1750    1989 
   Size:       1750     239
 
EcoICRI GAG'CTC

Cuts at:      0     834    1989 
   Size:        834    1155
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     102     152     850     979    1147    1678    1855    1858 
   Size:        102      50     698     129     168     531     177       3

Cuts at:   1858    1879    1989 
   Size:         21     110

  Fragments arranged by size: 

                 698     531     177     168     129     110     102      50
                  21       3
 
EcoO109I rG'GnC_Cy

Cuts at:      0     410     423     424     688     756    1183    1184    1335 
   Size:        410      13       1     264      68     427       1     151

Cuts at:   1335    1371    1484    1533    1989 
   Size:         36     113      49     456

  Fragments arranged by size: 

                 456     427     410     264     151     113      68      49
                  36      13       1       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     594    1989 
   Size:        594    1395
 
FspI TGC'GCA

Cuts at:      0     354    1948    1989 
   Size:        354    1594      41

  Fragments arranged by size: 

                1594     354      41
 
HaeII r_GCGC'y

Cuts at:      0     883    1989 
   Size:        883    1106
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      97     129    1384    1416    1989 
   Size:         97      32    1255      32     573

  Fragments arranged by size: 

                1255     573      97      32      32
 
HindIII A'AGCT_T

Cuts at:      0    1739    1989 
   Size:       1739     250
 
KasI G'GCGC_C

Cuts at:      0     879    1989 
   Size:        879    1110
 
MluI A'CGCG_T

Cuts at:      0     926    1146    1989 
   Size:        926     220     843

  Fragments arranged by size: 

                 926     843     220
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     848    1989 
   Size:        848    1141
 
MscI TGG'CCA

Cuts at:      0     610     979    1474    1989 
   Size:        610     369     495     515

  Fragments arranged by size: 

                 610     515     495     369
 
MslI CAynn'nnrTG

Cuts at:      0     334    1768    1942    1989 
   Size:        334    1434     174      47

  Fragments arranged by size: 

                1434     334     174      47
 
MspA1I CmG'CkG

Cuts at:      0     334    1272    1298    1826    1989 
   Size:        334     938      26     528     163

  Fragments arranged by size: 

                 938     528     334     163      26
 
NaeI GCC'GGC

Cuts at:      0    1321    1989 
   Size:       1321     668
 
NarI GG'CG_CC

Cuts at:      0     880    1989 
   Size:        880    1109
 
NcoI C'CATG_G

Cuts at:      0     488    1989 
   Size:        488    1501
 
NgoMIV G'CCGG_C

Cuts at:      0    1319    1989 
   Size:       1319     670
 
NsiI A_TGCA'T

Cuts at:      0    1636    1989 
   Size:       1636     353
 
NspI r_CATG'y

Cuts at:      0     540    1767    1989 
   Size:        540    1227     222

  Fragments arranged by size: 

                1227     540     222
 
PasI CC'CwG_GG

Cuts at:      0       6     136     214    1989 
   Size:          6     130      78    1775

  Fragments arranged by size: 

                1775     130      78       6
 
PciI A'CATG_T

Cuts at:      0     536    1989 
   Size:        536    1453
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     594    1165    1989 
   Size:        594     571     824

  Fragments arranged by size: 

                 824     594     571
 
PfoI T'CCnGG_A

Cuts at:      0     767    1502    1989 
   Size:        767     735     487

  Fragments arranged by size: 

                 767     735     487
 
PmlI CAC'GTG

Cuts at:      0     606    1413    1989 
   Size:        606     807     576

  Fragments arranged by size: 

                 807     606     576
 
PpuMI rG'GwC_Cy

Cuts at:      0     410     688    1484    1989 
   Size:        410     278     796     505

  Fragments arranged by size: 

                 796     505     410     278
 
PspOMI G'GGCC_C

Cuts at:      0     423    1183    1807    1989 
   Size:        423     760     624     182

  Fragments arranged by size: 

                 760     624     423     182
 
PspXI vC'TCGA_Gb

Cuts at:      0    1178    1989 
   Size:       1178     811
 
PstI C_TGCA'G

Cuts at:      0      91     533    1343    1989 
   Size:         91     442     810     646

  Fragments arranged by size: 

                 810     646     442      91
 
PvuII CAG'CTG

Cuts at:      0    1298    1989 
   Size:       1298     691
 
SacI G_AGCT'C

Cuts at:      0     836    1989 
   Size:        836    1153
 
SacII CC_GC'GG

Cuts at:      0     335    1989 
   Size:        335    1654
 
SanDI GG'GwC_CC

Cuts at:      0     688    1989 
   Size:        688    1301
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1714    1989 
   Size:       1714     275
 
SbfI CC_TGCA'GG

Cuts at:      0     533    1343    1989 
   Size:        533     810     646

  Fragments arranged by size: 

                 810     646     533
 
SfcI C'TryA_G

Cuts at:      0      87     529    1339    1989 
   Size:         87     442     810     650

  Fragments arranged by size: 

                 810     650     442      87
 
SfoI GGC'GCC

Cuts at:      0     881    1989 
   Size:        881    1108
 
SmaI CCC'GGG

Cuts at:      0     785    1989 
   Size:        785    1204
 
SmlI C'TyrA_G

Cuts at:      0    1178    1989 
   Size:       1178     811
 
StuI AGG'CCT

Cuts at:      0    1124    1989 
   Size:       1124     865
 
StyI C'CwwG_G

Cuts at:      0     488     569    1111    1989 
   Size:        488      81     542     878

  Fragments arranged by size: 

                 878     542     488      81
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      58     919    1989 
   Size:         58     861    1070

  Fragments arranged by size: 

                1070     861      58
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     584     616    1021    1053    1831    1856    1863    1888 
   Size:        584      32     405      32     778      25       7      25

Cuts at:   1888    1989 
   Size:        101

  Fragments arranged by size: 

                 778     584     405     101      32      32      25      25
                   7
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     204     618     810    1915    1989 
   Size:        204     414     192    1105      74

  Fragments arranged by size: 

                1105     414     204     192      74
 
XhoI C'TCGA_G

Cuts at:      0    1178    1989 
   Size:       1178     811
 
XmaI C'CCGG_G

Cuts at:      0     783    1989 
   Size:        783    1206
 
XmnI GAAnn'nnTTC

Cuts at:      0    1456    1989 
   Size:       1456     533
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AhdI     AleI     AlfI    AlwNI     ApaI
    ApaLI     AvaI     BanI    BanII     BbeI     BbsI     BcgI    BfrBI
     BglI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BsaAI
    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspHI    BspMI    BsrDI    BsrFI    BstBI   BstEII    BstXI   Bsu36I
     BtgI     BtsI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I     FalI     FspI    HaeII    Hin4I  HindIII     KasI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NsiI     NspI     PasI     PciI    PflMI     PfoI     PmlI
    PpuMI   PspOMI    PspXI     PstI    PvuII     SacI    SacII    SanDI
     SapI     SbfI     SfcI     SfoI     SmaI     SmlI     StuI     StyI
    TaqII     TstI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AloI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI
    BciVI     BclI    BglII     BlpI     BmtI    BpuEI     BsaI    BsaBI
    BsaWI    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrGI   BssHII
    BssSI   BstAPI    BstYI  BstZ17I    BtgZI     ClaI    CspCI     DraI
   DraIII    EcoNI    EcoRI    EcoRV     FseI    FspAI   HincII     HpaI
     KpnI     MfeI     NdeI     NheI     NotI     NruI     PacI     PmeI
     PpiI    PshAI     PsiI     PsrI     PvuI    RsrII     SalI     ScaI
    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     TatI  Tth111I     XbaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com