Restriction map for pF1KB6892
##################################
# NO CUT ENZYMES (  168 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BamHI       BanI        BbeI        
BbrPI       BbvCI       BclI        BfaI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstYI       BstZ17I     CelII       
ClaI        CpoI        DpnII       DraI        DsaI        
EagI        Eam1105I    EclXI       Eco52I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        FspI        HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MaeI        MaeII       MaeIII      MamI        MboI        
MfeI        MflI        MluI        MroI        MslI        
MspA1I      MunI        MvnI        NarI        NcoI        
NdeI        NdeII       NheI        NlaIII      NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        Sau3AI      SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StyI        SwaI        TaqI        Tsp45I      Tsp509I     
Tth111I     TthHB8I     XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AcyI          486  GACGCC               G[GA]^CG[CT]C
AfaI          559  GTAC                 GT^AC
AocI          591  CCTCAGG              CC^T[ACGT]AGG
Aor51HI       263  AGCGCT               AGC^GCT
ApaI          477  GGGCCC               GGGCC^C
BalI          320  TGGCCA               TGG^CCA
BbsI           88  GTCTTC               GAAGAC(2/6)
BcgI          485  CGACGCCCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI          54  GGATAC               GTATCC(6/5)
BglI          322  GCCAGCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          163  CTGGAG               CTGGAG(16/14)
BpuAI          88  GTCTTC               GAAGAC(2/6)
BsaHI         486  GACGCC               G[GA]^CG[CT]C
BseRI          70  GAGGAG               GAGGAG(10/8)
BsmAI          73  GAGAC                GTCTC(1/5)
BspMI          46  ACCTGC               ACCTGC(4/8)
BsrFI         370  GCCGGC               [GA]^CCGG[CT]
BstXI          44  CCACCTGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        591  CCTCAGG              CC^T[ACGT]AGG
Cfr10I        370  GCCGGC               [GA]^CCGG[CT]
DraIII         62  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI           79  GACCAAGAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      263  AGCGCT               AGC^GCT
Eco81I        591  CCTCAGG              CC^T[ACGT]AGG
FseI          369  GGCCGGCC             GGCCGG^CC
Hin1I         486  GACGCC               G[GA]^CG[CT]C
HphI           61  TCACC                GGTGA(8/7)
MluNI         320  TGGCCA               TGG^CCA
MscI          320  TGGCCA               TGG^CCA
MseI           40  TTAA                 T^TAA
NaeI          370  GCCGGC               GCC^GGC
NgoMIV        370  GCCGGC               G^CCGGC
PflMI         149  CCAACCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          459  GAGTC                GAGTC(4/5)
RsaI          559  GTAC                 GT^AC
SfaNI         350  GCATC                GCATC(5/9)
TfiI          138  GATTC                G^A[AT]TC
Tru9I          40  TTAA                 T^TAA
Van91I        149  CCAACCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          323  CCAGCCTGGCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AlwNI         538  CAGGCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              548  CAGCCTCTG           
AvaII          97  GGTCC                G^G[AT]CC
              159  GGACC               
BanII         442  GGGCTC               G[GA]GC[CT]^C
              477  GGGCCC              
BcnI          134  CCCGG                CC^[GC]GG
              440  CCGGG               
BmyI          442  GGGCTC               G[AGT]GC[ACT]^C
              477  GGGCCC              
Bsp1286I      442  GGGCTC               G[AGT]GC[ACT]^C
              477  GGGCCC              
BsrI          155  ACTGG                ACTGG(1/-1)
               65  CCAGT               
BstF5I        351  CATCC                GGATG(2/0)
              315  GGATG               
DraII          96  GGGTCCC              [GA]G^G[ACGT]CC[CT]
              158  GGGACCT             
EaeI          320  TGGCCA               [CT]^GGCC[GA]
              368  TGGCCG              
EarI          205  GAAGAG               CTCTTC(1/4)
              223  GAAGAG              
EcoO109I       96  GGGTCCC              [GA]G^G[ACGT]CC[CT]
              158  GGGACCT             
FokI          351  CATCC                GGATG(9/13)
              315  GGATG               
HaeII         263  AGCGCT               [GA]GCGC^[CT]
              540  GGCGCT              
HgaI          486  GACGC                GACGC(5/10)
              565  GACGC               
HinfI         138  GATTC                G^A[ACGT]TC
              459  GAGTC               
Ksp632I       205  GAAGAG               CTCTTC(1/4)
              223  GAAGAG              
MwoI          322  GCCAGCCTGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              541  GCGCTGGCAGC         
NciI          134  CCCGG                CC^[GC]GG
              440  CCGGG               
PpuMI          96  GGGTCCC              [GA]G^G[AT]CC[CT]
              158  GGGACCT             
StuI          519  AGGCCT               AGG^CCT
              598  AGGCCT              
TspRI          66  CAGTG                CA[GC]TG[ACGT][ACGT]^
              154  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AluI        BbvI        BsaJI       BsiYI       BslI        
BsmFI       BssKI       BstNI       Cac8I       CfoI        
Cfr13I      DdeI        EcoRII      Fnu4HI      HaeIII      
HapII       HhaI        HinP1I      HpaII       ItaI        
MboII       MnlI        MspI        MvaI        NlaIV       
Sau96I      ScrFI       TseI        
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