Restriction map for
pF1KB6289
(Linear) (Six-base) MAPSORT of: KIBB6289.seq Check: 1221 from: 1 to: 2289
KIBB6289.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AatII G_ACGT'C
Cuts at: 0 898 2096 2289
Size: 898 1198 193
Fragments arranged by size:
1198 898 193
Acc65I G'GTAC_C
Cuts at: 0 1134 2289
Size: 1134 1155
AflIII A'CryG_T
Cuts at: 0 878 2289
Size: 878 1411
AhdI GACnn_n'nnGTC
Cuts at: 0 987 2289
Size: 987 1302
AleI CACnn'nnGTG
Cuts at: 0 935 2289
Size: 935 1354
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 537 2218 2289
Size: 537 1681 71
Fragments arranged by size:
1681 537 71
AlwNI CAG_nnn'CTG
Cuts at: 0 1164 2289
Size: 1164 1125
ApaI G_GGCC'C
Cuts at: 0 308 1697 2248 2289
Size: 308 1389 551 41
Fragments arranged by size:
1389 551 308 41
ApoI r'AATT_y
Cuts at: 0 313 2289
Size: 313 1976
AvaI C'yCGr_G
Cuts at: 0 1003 1833 2289
Size: 1003 830 456
Fragments arranged by size:
1003 830 456
BanI G'GyrC_C
Cuts at: 0 1134 1188 1933 2289
Size: 1134 54 745 356
Fragments arranged by size:
1134 745 356 54
BanII G_rGCy'C
Cuts at: 0 308 342 474 589 1046 1222 1444 1697
Size: 308 34 132 115 457 176 222 253
Cuts at: 1697 1724 2248 2289
Size: 27 524 41
Fragments arranged by size:
524 457 308 253 222 176 132 115
41 34 27
BbvCI CC'TCA_GC
Cuts at: 0 1277 2191 2289
Size: 1277 914 98
Fragments arranged by size:
1277 914 98
BlpI GC'TnA_GC
Cuts at: 0 1762 2289
Size: 1762 527
Bme1580I G_kGCm'C
Cuts at: 0 308 1697 2248 2289
Size: 308 1389 551 41
Fragments arranged by size:
1389 551 308 41
BmrI ACTGGGnnnn_n'
Cuts at: 0 592 929 1691 1909 2251 2262 2289
Size: 592 337 762 218 342 11 27
Fragments arranged by size:
762 592 342 337 218 27 11
BmtI G_CTAG'C
Cuts at: 0 1276 2289
Size: 1276 1013
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 177 1479 1646 2289
Size: 177 1302 167 643
Fragments arranged by size:
1302 643 177 167
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 433 488 655 1890 2289
Size: 338 95 55 167 1235 399
Fragments arranged by size:
1235 399 338 167 95 55
Bpu10I CC'TnA_GC
Cuts at: 0 1277 1558 2191 2279 2289
Size: 1277 281 633 88 10
Fragments arranged by size:
1277 633 281 88 10
BsaI GGTCTCn'nnnn_
Cuts at: 0 409 487 663 918 995 1416 1634 2289
Size: 409 78 176 255 77 421 218 655
Fragments arranged by size:
655 421 409 255 218 176 78 77
BsaAI yAC'GTr
Cuts at: 0 791 2289
Size: 791 1498
BsaBI GATnn'nnATC
Cuts at: 0 449 2289
Size: 449 1840
BsaHI Gr'CG_yC
Cuts at: 0 895 2093 2289
Size: 895 1198 196
Fragments arranged by size:
1198 895 196
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 336 366 1498 1528 1738 1768 2038 2068
Size: 336 30 1132 30 210 30 270 30
Cuts at: 2068 2289
Size: 221
Fragments arranged by size:
1132 336 270 221 210 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 100 673 1283 1294 1336 1626 1635 1731
Size: 100 573 610 11 42 290 9 96
Cuts at: 1731 1815 2017 2289
Size: 84 202 272
Fragments arranged by size:
610 573 290 272 202 100 96 84
42 11 9
BseYI C'CCAG_C
Cuts at: 0 178 1709 1731 1757 1884 2289
Size: 178 1531 22 26 127 405
Fragments arranged by size:
1531 405 178 127 26 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 507 521 856 2289
Size: 507 14 335 1433
Fragments arranged by size:
1433 507 335 14
BsiEI CG_ry'CG
Cuts at: 0 1033 2289
Size: 1033 1256
BsiHKAI G_wGCw'C
Cuts at: 0 1010 1046 1222 1724 2001 2289
Size: 1010 36 176 502 277 288
Fragments arranged by size:
1010 502 288 277 176 36
BsmBI CGTCTCn'nnnn_
Cuts at: 0 886 2289
Size: 886 1403
Bsp1286I G_dGCh'C
Cuts at: 0 308 342 474 589 1010 1046 1222 1444
Size: 308 34 132 115 421 36 176 222
Cuts at: 1444 1697 1724 2001 2248 2289
Size: 253 27 277 247 41
Fragments arranged by size:
421 308 277 253 247 222 176 132
115 41 36 34 27
BspHI T'CATG_A
Cuts at: 0 1214 2289
Size: 1214 1075
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 441 2289
Size: 441 1848
BsrBI CCG'CTC
Cuts at: 0 705 2289
Size: 705 1584
BsrDI GCAATG_nn'
Cuts at: 0 824 1192 1253 2289
Size: 824 368 61 1036
Fragments arranged by size:
1036 824 368 61
BsrFI r'CCGG_y
Cuts at: 0 1625 2289
Size: 1625 664
BstAPI GCAn_nnn'nTGC
Cuts at: 0 274 1650 2289
Size: 274 1376 639
Fragments arranged by size:
1376 639 274
BstBI TT'CG_AA
Cuts at: 0 561 2289
Size: 561 1728
BstXI CCAn_nnnn'nTGG
Cuts at: 0 147 1434 2289
Size: 147 1287 855
Fragments arranged by size:
1287 855 147
BstYI r'GATC_y
Cuts at: 0 100 1971 2198 2289
Size: 100 1871 227 91
Fragments arranged by size:
1871 227 100 91
BtgI C'CryG_G
Cuts at: 0 1114 2289
Size: 1114 1175
BtsI GCAGTG_nn'
Cuts at: 0 1105 2289
Size: 1105 1184
DrdI GACnn_nn'nnGTC
Cuts at: 0 883 2289
Size: 883 1406
EaeI y'GGCC_r
Cuts at: 0 19 227 706 725 2289
Size: 19 208 479 19 1564
Fragments arranged by size:
1564 479 208 19 19
EarI CTCTTCn'nnn_
Cuts at: 0 1448 1687 2079 2289
Size: 1448 239 392 210
Fragments arranged by size:
1448 392 239 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 844 1036 1107 2289
Size: 844 192 71 1182
Fragments arranged by size:
1182 844 192 71
EcoICRI GAG'CTC
Cuts at: 0 1044 1220 1722 2289
Size: 1044 176 502 567
Fragments arranged by size:
1044 567 502 176
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 433 488 655 1890 2289
Size: 338 95 55 167 1235 399
Fragments arranged by size:
1235 399 338 167 95 55
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2230 2289
Size: 2230 59
EcoO109I rG'GnC_Cy
Cuts at: 0 1562 1693 1786 2235 2245 2289
Size: 1562 131 93 449 10 44
Fragments arranged by size:
1562 449 131 93 44 10
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 933 2289
Size: 933 1356
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 973 1005 1278 1310 1477 1498 1509 1530
Size: 973 32 273 32 167 21 11 21
Cuts at: 1530 1738 1770 2038 2070 2289
Size: 208 32 268 32 219
Fragments arranged by size:
973 273 268 219 208 167 32 32
32 32 21 21 11
KpnI G_GTAC'C
Cuts at: 0 1138 2289
Size: 1138 1151
MfeI C'AATT_G
Cuts at: 0 997 2289
Size: 997 1292
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 569 2289
Size: 182 387 1720
Fragments arranged by size:
1720 387 182
MscI TGG'CCA
Cuts at: 0 229 727 2289
Size: 229 498 1562
Fragments arranged by size:
1562 498 229
MslI CAynn'nnrTG
Cuts at: 0 727 935 1432 2289
Size: 727 208 497 857
Fragments arranged by size:
857 727 497 208
MspA1I CmG'CkG
Cuts at: 0 178 329 1709 2289
Size: 178 151 1380 580
Fragments arranged by size:
1380 580 178 151
NheI G'CTAG_C
Cuts at: 0 1272 2289
Size: 1272 1017
NspI r_CATG'y
Cuts at: 0 882 2289
Size: 882 1407
PasI CC'CwG_GG
Cuts at: 0 985 1080 1929 1959 2289
Size: 985 95 849 30 330
Fragments arranged by size:
985 849 330 95 30
PciI A'CATG_T
Cuts at: 0 878 2289
Size: 878 1411
PflMI CCAn_nnn'nTGG
Cuts at: 0 1164 1779 2289
Size: 1164 615 510
Fragments arranged by size:
1164 615 510
PfoI T'CCnGG_A
Cuts at: 0 354 887 1867 2168 2289
Size: 354 533 980 301 121
Fragments arranged by size:
980 533 354 301 121
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1711 1743 2289
Size: 1711 32 546
Fragments arranged by size:
1711 546 32
PpuMI rG'GwC_Cy
Cuts at: 0 1562 1786 2235 2289
Size: 1562 224 449 54
Fragments arranged by size:
1562 449 224 54
PspOMI G'GGCC_C
Cuts at: 0 304 1693 2244 2289
Size: 304 1389 551 45
Fragments arranged by size:
1389 551 304 45
PstI C_TGCA'G
Cuts at: 0 438 1308 2289
Size: 438 870 981
Fragments arranged by size:
981 870 438
PvuII CAG'CTG
Cuts at: 0 178 329 2289
Size: 178 151 1960
Fragments arranged by size:
1960 178 151
SacI G_AGCT'C
Cuts at: 0 1046 1222 1724 2289
Size: 1046 176 502 565
Fragments arranged by size:
1046 565 502 176
SapI GCTCTTCn'nnn_
Cuts at: 0 1687 2289
Size: 1687 602
ScaI AGT'ACT
Cuts at: 0 1701 2289
Size: 1701 588
SexAI A'CCwGG_T
Cuts at: 0 962 1130 2289
Size: 962 168 1159
Fragments arranged by size:
1159 962 168
SfcI C'TryA_G
Cuts at: 0 434 1304 1548 2284 2289
Size: 434 870 244 736 5
Fragments arranged by size:
870 736 434 244 5
SspI AAT'ATT
Cuts at: 0 249 2289
Size: 249 2040
StuI AGG'CCT
Cuts at: 0 136 2162 2289
Size: 136 2026 127
Fragments arranged by size:
2026 136 127
StyI C'CwwG_G
Cuts at: 0 140 278 419 1262 1448 2289
Size: 140 138 141 843 186 841
Fragments arranged by size:
843 841 186 141 140 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1047 1815 2289
Size: 1047 768 474
Fragments arranged by size:
1047 768 474
TatI w'GTAC_w
Cuts at: 0 1699 1917 2289
Size: 1699 218 372
Fragments arranged by size:
1699 372 218
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 850 882 2289
Size: 850 32 1407
Fragments arranged by size:
1407 850 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 680 2289
Size: 680 1609
ZraI GAC'GTC
Cuts at: 0 896 2094 2289
Size: 896 1198 195
Fragments arranged by size:
1198 896 195
Enzymes that do cut:
AatII Acc65I AflIII AhdI AleI AlfI AlwNI ApaI
ApoI AvaI BanI BanII BbvCI BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstBI BstXI
BstYI BtgI BtsI DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI Hin4I KpnI MfeI MmeI
MscI MslI MspA1I NheI NspI PasI PciI PflMI
PfoI PpiI PpuMI PspOMI PstI PvuII SacI SapI
ScaI SexAI SfcI SspI StuI StyI TaqII TatI
TstI XcmI ZraI
Enzymes that do not cut:
AarI AccI AclI AcuI AfeI AflII AgeI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BmgBI
BpuEI BsaWI BsiWI BsmI BspEI BsrGI BssHII BssSI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
EagI EcoRI EcoRV FseI FspI FspAI HaeII HincII
HindIII HpaI KasI MluI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NsiI PacI PmeI PmlI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SwaI Tth111I XbaI XhoI XmaI
XmnI