Restriction map for pF1KB6279
##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

AatII       AccII       AccIII      AcyI        AflII       
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaLI       AscI        AspEI       AspI        AviII       
AvrII       BamHI       BbeI        BbrPI       BcgI        
BciVI       BcnI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmBI       Bsp1407I    BspDI       
BspEI       BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstUI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraI        DraIII      EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoT22I     
FseI        FspI        HaeII       HapII       Hin1I       
HincII      HindII      HpaI        HpaII       KasI        
KspI        MamI        MfeI        MluI        MroI        
MspI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PvuI        RsrII       
SacII       SalI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tth111I     Van91I      XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

Acc65I       1474  GGTACC               G^GTACC
AclI          443  AACGTT               AA^CGTT
ApaI         1357  GGGCCC               GGGCC^C
AseI         1183  ATTAAT               AT^TAAT
AsnI         1183  ATTAAT               AT^TAAT
Asp718I      1474  GGTACC               G^GTACC
AvaI         1875  CCCGAG               C^[CT]CG[GA]G
BanI         1474  GGTACC               G^G[CT][GA]CC
BbvCI        2143  GCTGAGG              CCTCAGC(-5/-2)
BclI         1332  TGATCA               T^GATCA
BseRI         250  GAGGAG               GAGGAG(10/8)
BsmI         1924  GCATTC               GAATGC(1/-1)
BsoBI        1875  CCCGAG               C^[CT]CG[GA]G
BstAPI       1084  GCAGAATATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1740  GGTCACC              G^GT[ACGT]ACC
BstPI        1740  GGTCACC              G^GT[ACGT]ACC
BstYI         167  AGATCC               [GA]^GATC[CT]
CfoI         1462  GCGC                 GCG^C
DraII        1356  GGGGCCC              [GA]G^G[ACGT]CC[CT]
DrdI         1939  GACAAGCAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         1538  CCACGG               C^C[GA][CT]GG
EcoO109I     1356  GGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoO65I      1740  GGTCACC              G^GT[ACGT]ACC
EcoRI        1989  GAATTC               G^AATTC
EcoT14I      2309  CCTTGG               C^C[AT][AT]GG
FauI          451  CCCGC                CCCGC(4/6)
FbaI         1332  TGATCA               T^GATCA
HgaI         1141  GCGTC                GACGC(5/10)
HhaI         1462  GCGC                 GCG^C
HinP1I       1462  GCGC                 G^CGC
HindIII      2165  AAGCTT               A^AGCTT
KpnI         1474  GGTACC               GGTAC^C
MflI          167  AGATCC               [GA]^GATC[CT]
MspA1I       1508  CAGCTG               C[AC]G^C[GT]G
PshBI        1183  ATTAAT               AT^TAAT
Psp1406I      443  AACGTT               AA^CGTT
PvuII        1508  CAGCTG               CAG^CTG
SacI         2178  GAGCTC               GAGCT^C
SapI          271  GAAGAGC              GCTCTTC(1/4)
SmlI          302  CTCAAG               C^T[CT][GA]AG
StyI         2309  CCTTGG               C^C[AT][AT]GG
XcmI         1647  CCACAAAGCTAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         167  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccI          105  GTCTAC               GT^[AC][GT]AC
              412  GTAGAC              
AflIII        564  ACATGT               A^C[GA][CT]GT
             1799  ACATGT              
Asp700I       292  GAAAGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1985  GAATGAATTC          
AvaII         243  GGTCC                G^G[AT]CC
             2275  GGACC               
BalI           99  TGGCCA               TGG^CCA
             2367  TGGCCA              
BbsI           63  GTCTTC               GAAGAC(2/6)
              568  GTCTTC              
BfaI         1706  CTAG                 C^TAG
             1847  CTAG                
BpuAI          63  GTCTTC               GAAGAC(2/6)
              568  GTCTTC              
BsiYI        2062  CCCTTTTCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             2206  CCCTATCATGG         
BslI         2062  CCCTTTTCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             2206  CCCTATCATGG         
BspHI         840  TCATGA               T^CATGA
             1590  TCATGA              
BspLU11I      564  ACATGT               A^CATGT
             1799  ACATGT              
BspMI         635  ACCTGC               ACCTGC(4/8)
             1944  GCAGGT              
BsrDI         527  GCAATG               GCAATG(2/0)
             1319  GCAATG              
BstXI          68  CCAGGACAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2370  CCAGACTTTTGG        
EaeI           99  TGGCCA               [CT]^GGCC[GA]
             2367  TGGCCA              
EcoRV         222  GATATC               GAT^ATC
             1671  GATATC              
MaeI         1706  CTAG                 C^TAG
             1847  CTAG                
MluNI          99  TGGCCA               TGG^CCA
             2367  TGGCCA              
MscI           99  TGGCCA               TGG^CCA
             2367  TGGCCA              
NdeI          721  CATATG               CA^TATG
             1247  CATATG              
RcaI          840  TCATGA               T^CATGA
             1590  TCATGA              
TaqI          147  TCGA                 T^CGA
             1977  TCGA                
TthHB8I       147  TCGA                 T^CGA
             1977  TCGA                
XmnI          292  GAAAGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1985  GAATGAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AspHI       BanII       BbvI        
BmyI        BpmI        BsaJI       BsiHKAI     BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DpnII       
EarI        EcoRII      Fnu4HI      FokI        HaeIII      
HinfI       HphI        ItaI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MnlI        MseI        
MslI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PleI        PstI        RsaI        
Sau3AI      Sau96I      ScaI        ScrFI       SfaNI       
SfcI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com