Restriction map for pF1KB6250
##################################
# NO CUT ENZYMES (  145 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AciI        
AclI        AcsI        AcyI        AflII       AgeI        
AhdI        Alw44I      AlwNI       ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        AspEI       
AvaI        AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaJI       BseAI       BsiEI       BsiWI       BsmBI       
BsmFI       BsmI        BsoBI       BspDI       BspEI       
BsrBI       BsrFI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstZ17I     CelII       Cfr10I      ClaI        CpoI        
DraI        DraII       DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco52I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
FauI        FbaI        FseI        FspI        HgaI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KspI        MaeI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    StyI        SwaI        Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

Acc65I        284  GGTACC               G^GTACC
AflIII       1426  ACATGT               A^C[GA][CT]GT
AlwI         1787  GATCC                GGATC(4/5)
AocI          230  CCTCAGG              CC^T[ACGT]AGG
Aor51HI      1772  AGCGCT               AGC^GCT
Asp718I       284  GGTACC               G^GTACC
AspI            3  GACGAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BcnI         1194  CCGGG                CC^[GC]GG
BsaWI        1903  ACCGGA               [AT]^CCGG[AT]
BsgI         1589  GTGCAG               GTGCAG(16/14)
Bsp1407I     1464  TGTACA               T^GTACA
BspLU11I     1426  ACATGT               A^CATGT
BspMI         287  ACCTGC               ACCTGC(4/8)
BsrDI        1001  GCAATG               GCAATG(2/0)
BsrGI        1464  TGTACA               T^GTACA
BstF5I        197  GGATG                GGATG(2/0)
BstNI        1362  CCAGG                CC^[AT]GG
BstXI        1706  CCAATTTTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        230  CCTCAGG              CC^T[ACGT]AGG
Cac8I         957  GCAGGC               GC[ACGT]^[ACGT]GC
CfoI         1773  GCGC                 GCG^C
DraIII       1718  CACATCGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III     1772  AGCGCT               AGC^GCT
Eco81I        230  CCTCAGG              CC^T[ACGT]AGG
EcoRII       1362  CCAGG                ^CC[AT]GG
FokI          197  GGATG                GGATG(9/13)
HaeII        1772  AGCGCT               [GA]GCGC^[CT]
HhaI         1773  GCGC                 GCG^C
HinP1I       1773  GCGC                 G^CGC
KpnI          284  GGTACC               GGTAC^C
MaeII          35  ACGT                 A^CGT
MvaI         1362  CCAGG                CC^[AT]GG
MwoI           63  GCTGCAACTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI         1194  CCGGG                CC^[GC]GG
PflFI           3  GACGAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI          955  CTGCAG               CTGCA^G
SmlI         1956  CTCAAG               C^T[CT][GA]AG
SspBI        1464  TGTACA               T^GTACA
SspI          370  AATATT               AAT^ATT
TaqI         2043  TCGA                 T^CGA
Tth111I         3  GACGAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I      2043  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

BanI          284  GGTACC               G^G[CT][GA]CC
             1643  GGTGCC              
BbvI           63  GCTGC                GCAGC(8/12)
              386  GCTGC               
BglII         317  AGATCT               A^GATCT
             1455  AGATCT              
BsaI         1827  GAGACC               GGTCTC(1/5)
              132  GGTCTC              
BspHI         294  TCATGA               T^CATGA
              963  TCATGA              
BssKI        1194  CCGGG                ^CC[ACGT]GG
             1362  CCAGG               
EcoT22I      1004  ATGCAT               ATGCA^T
             1256  ATGCAT              
Fnu4HI         63  GCTGC                GC^[ACGT]GC
              386  GCTGC               
HapII        1194  CCGG                 C^CGG
             1904  CCGG                
HpaII        1194  CCGG                 C^CGG
             1904  CCGG                
HphI          478  GGTGA                GGTGA(8/7)
             1999  GGTGA               
ItaI           63  GCTGC                GC^[ACGT]GC
              386  GCTGC               
MspA1I        519  CAGCTG               C[AC]G^C[GT]G
             1863  CAGCTG              
MspI         1194  CCGG                 C^CGG
             1904  CCGG                
NsiI         1004  ATGCAT               ATGCA^T
             1256  ATGCAT              
PvuII         519  CAGCTG               CAG^CTG
             1863  CAGCTG              
RcaI          294  TCATGA               T^CATGA
              963  TCATGA              
SapI          298  GAAGAGC              GCTCTTC(1/4)
              930  GAAGAGC             
ScrFI        1194  CCGGG                CC^[ACGT]GG
             1362  CCAGG               
SfcI          955  CTGCAG               C^T[GA][CT]AG
             1470  CTGTAG              
StuI         1364  AGGCCT               AGG^CCT
             1520  AGGCCT              
TseI           63  GCTGC                G^C[AT]GC
              386  GCTGC               
Tsp509I       540  AATT                 ^AATT
             1708  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AfaI        AluI        Asp700I     AspHI       AvaII       
BanII       BbsI        BmyI        BpmI        BpuAI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
Bsp1286I    BsrI        BstYI       Cfr13I      DdeI        
DpnII       EarI        HaeIII      HinfI       Ksp632I     
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        NdeII       NlaIII      NlaIV       
NspI        PleI        RsaI        SacI        Sau3AI      
Sau96I      SfaNI       TfiI        Tru9I       Tsp45I      
TspRI       XhoII       XmnI        
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