Restriction map for pF1KB6227
 (Linear) (Six-base) MAPSORT of: KIBB6227.seq  Check: 5240  from: 1  to: 2016



KIBB6227.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     409    2016 
   Size:        409    1607
 
AclI AA'CG_TT

Cuts at:      0     413    1661    2016 
   Size:        413    1248     355

  Fragments arranged by size: 

                1248     413     355
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     683     983    1129    1411    2016 
   Size:        683     300     146     282     605

  Fragments arranged by size: 

                 683     605     300     282     146
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1903    2016 
   Size:       1903     113
 
AlwNI CAG_nnn'CTG

Cuts at:      0     155     449    2016 
   Size:        155     294    1567

  Fragments arranged by size: 

                1567     294     155
 
ApaLI G'TGCA_C

Cuts at:      0      91    1146    1990    2016 
   Size:         91    1055     844      26

  Fragments arranged by size: 

                1055     844      91      26
 
ApoI r'AATT_y

Cuts at:      0     112     238     910    1300    2016 
   Size:        112     126     672     390     716

  Fragments arranged by size: 

                 716     672     390     126     112
 
AvaI C'yCGr_G

Cuts at:      0      15    2016 
   Size:         15    2001
 
BamHI G'GATC_C

Cuts at:      0     558     769    2016 
   Size:        558     211    1247

  Fragments arranged by size: 

                1247     558     211
 
BanI G'GyrC_C

Cuts at:      0     230    2016 
   Size:        230    1786
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     971    1634    2016 
   Size:        971     663     382

  Fragments arranged by size: 

                 971     663     382
 
BbvCI CC'TCA_GC

Cuts at:      0      76     497    2016 
   Size:         76     421    1519

  Fragments arranged by size: 

                1519     421      76
 
BclI T'GATC_A

Cuts at:      0    1920    2016 
   Size:       1920      96
 
BglI GCCn_nnn'nGGC

Cuts at:      0     938    2016 
   Size:        938    1078
 
Bme1580I G_kGCm'C

Cuts at:      0      95    1081    1150    1994    2016 
   Size:         95     986      69     844      22

  Fragments arranged by size: 

                 986     844      95      69      22
 
BmgBI CAC'GTC

Cuts at:      0      45    1140    1770    2016 
   Size:         45    1095     630     246

  Fragments arranged by size: 

                1095     630     246      45
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     747    1812    1977    2016 
   Size:        747    1065     165      39

  Fragments arranged by size: 

                1065     747     165      39
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1021    2016 
   Size:       1021     995
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1487    1834    2016 
   Size:       1487     347     182

  Fragments arranged by size: 

                1487     347     182
 
Bpu10I CC'TnA_GC

Cuts at:      0      76     497    2016 
   Size:         76     421    1519

  Fragments arranged by size: 

                1519     421      76
 
BsaBI GATnn'nnATC

Cuts at:      0    1342    2016 
   Size:       1342     674
 
BsaHI Gr'CG_yC

Cuts at:      0      34    1574    2016 
   Size:         34    1540     442

  Fragments arranged by size: 

                1540     442      34
 
BsaWI w'CCGG_w

Cuts at:      0     870    1790    2016 
   Size:        870     920     226

  Fragments arranged by size: 

                 920     870     226
 
BseYI C'CCAG_C

Cuts at:      0     442    1728    1740    2016 
   Size:        442    1286      12     276

  Fragments arranged by size: 

                1286     442     276      12
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     141    1192    2016 
   Size:        141    1051     824

  Fragments arranged by size: 

                1051     824     141
 
BsiHKAI G_wGCw'C

Cuts at:      0      95    1150    1658    1994    2016 
   Size:         95    1055     508     336      22

  Fragments arranged by size: 

                1055     508     336      95      22
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      41    2016 
   Size:         41    1975
 
Bsp1286I G_dGCh'C

Cuts at:      0      95    1081    1150    1658    1994    2016 
   Size:         95     986      69     508     336      22

  Fragments arranged by size: 

                 986     508     336      95      69      22
 
BspEI T'CCGG_A

Cuts at:      0     870    1790    2016 
   Size:        870     920     226

  Fragments arranged by size: 

                 920     870     226
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     409    2016 
   Size:        409    1607
 
BsrBI CCG'CTC

Cuts at:      0     647    2016 
   Size:        647    1369
 
BsrDI GCAATG_nn'

Cuts at:      0     430    2016 
   Size:        430    1586
 
BsrFI r'CCGG_y

Cuts at:      0     807    2016 
   Size:        807    1209
 
BssHII G'CGCG_C

Cuts at:      0     121    2016 
   Size:        121    1895
 
BssSI C'ACGA_G

Cuts at:      0      94    1892    2016 
   Size:         94    1798     124

  Fragments arranged by size: 

                1798     124      94
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     155    2016 
   Size:        155    1861
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1740    2016 
   Size:       1740     276
 
BstYI r'GATC_y

Cuts at:      0     558     576     769    1386    1826    2016 
   Size:        558      18     193     617     440     190

  Fragments arranged by size: 

                 617     558     440     193     190      18
 
Bsu36I CC'TnA_GG

Cuts at:      0    1754    2016 
   Size:       1754     262
 
BtgI C'CryG_G

Cuts at:      0      29    1151    2016 
   Size:         29    1122     865

  Fragments arranged by size: 

                1122     865      29
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     102    2016 
   Size:        102    1914
 
BtsI GCAGTG_nn'

Cuts at:      0     153     358     670    2016 
   Size:        153     205     312    1346

  Fragments arranged by size: 

                1346     312     205     153
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1836    1871    2016 
   Size:       1836      35     145

  Fragments arranged by size: 

                1836     145      35
 
DraIII CAC_nnn'GTG

Cuts at:      0      99    2016 
   Size:         99    1917
 
EaeI y'GGCC_r

Cuts at:      0      47     809    2016 
   Size:         47     762    1207

  Fragments arranged by size: 

                1207     762      47
 
EarI CTCTTCn'nnn_

Cuts at:      0     466    2016 
   Size:        466    1550
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     495    2016 
   Size:        495    1521
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     683     983    1129    1411    1487    1834    2016 
   Size:        683     300     146     282      76     347     182

  Fragments arranged by size: 

                 683     347     300     282     182     146      76
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1111    2016 
   Size:       1111     905
 
EcoO109I rG'GnC_Cy

Cuts at:      0     274    1269    1487    2016 
   Size:        274     995     218     529

  Fragments arranged by size: 

                 995     529     274     218
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     943    1185    2016 
   Size:        943     242     831

  Fragments arranged by size: 

                 943     831     242
 
HaeII r_GCGC'y

Cuts at:      0      76    2016 
   Size:         76    1940
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1215    1247    1889    1921    2016 
   Size:       1215      32     642      32      95

  Fragments arranged by size: 

                1215     642      95      32      32
 
HincII GTy'rAC

Cuts at:      0    1260    1977    2016 
   Size:       1260     717      39

  Fragments arranged by size: 

                1260     717      39
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1007    1391    2016 
   Size:       1007     384     625

  Fragments arranged by size: 

                1007     625     384
 
MscI TGG'CCA

Cuts at:      0      49    2016 
   Size:         49    1967
 
MspA1I CmG'CkG

Cuts at:      0    1206    1926    2016 
   Size:       1206     720      90

  Fragments arranged by size: 

                1206     720      90
 
NaeI GCC'GGC

Cuts at:      0     809    2016 
   Size:        809    1207
 
NcoI C'CATG_G

Cuts at:      0    1151    2016 
   Size:       1151     865
 
NgoMIV G'CCGG_C

Cuts at:      0     807    2016 
   Size:        807    1209
 
NspI r_CATG'y

Cuts at:      0    1783    2016 
   Size:       1783     233
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     818    1227    1615    2016 
   Size:        818     409     388     401

  Fragments arranged by size: 

                 818     409     401     388
 
PpuMI rG'GwC_Cy

Cuts at:      0     274    1269    1487    2016 
   Size:        274     995     218     529

  Fragments arranged by size: 

                 995     529     274     218
 
PshAI GACnn'nnGTC

Cuts at:      0     717    2016 
   Size:        717    1299
 
PstI C_TGCA'G

Cuts at:      0     151    2016 
   Size:        151    1865
 
PvuII CAG'CTG

Cuts at:      0    1206    1926    2016 
   Size:       1206     720      90

  Fragments arranged by size: 

                1206     720      90
 
SanDI GG'GwC_CC

Cuts at:      0     274    2016 
   Size:        274    1742
 
ScaI AGT'ACT

Cuts at:      0     853    2016 
   Size:        853    1163
 
SfcI C'TryA_G

Cuts at:      0     147     722    2016 
   Size:        147     575    1294

  Fragments arranged by size: 

                1294     575     147
 
SmaI CCC'GGG

Cuts at:      0      17    2016 
   Size:         17    1999
 
SphI G_CATG'C

Cuts at:      0    1783    2016 
   Size:       1783     233
 
StyI C'CwwG_G

Cuts at:      0    1151    1685    2016 
   Size:       1151     534     331

  Fragments arranged by size: 

                1151     534     331
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     725    1010    1742    2016 
   Size:        725     285     732     274

  Fragments arranged by size: 

                 732     725     285     274
 
TatI w'GTAC_w

Cuts at:      0     851    1238    2016 
   Size:        851     387     778

  Fragments arranged by size: 

                 851     778     387
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     378     410     642     674    1479    1511    1538    1570 
   Size:        378      32     232      32     805      32      27      32

Cuts at:   1570    1648    1680    2016 
   Size:         78      32     336

  Fragments arranged by size: 

                 805     378     336     232      78      32      32      32
                  32      32      27
 
XmaI C'CCGG_G

Cuts at:      0      15    2016 
   Size:         15    2001
 
XmnI GAAnn'nnTTC

Cuts at:      0     760    2016 
   Size:        760    1256
 

 Enzymes that do cut:

     AarI     AclI     AcuI     AhdI    AlwNI    ApaLI     ApoI     AvaI
    BamHI     BanI     BbsI    BbvCI     BclI     BglI Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BsaBI    BsaHI    BsaWI    BseYI
     BsgI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI
    BsrFI   BssHII    BssSI   BstAPI    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI    CspCI   DraIII     EaeI     EarI     EciI  Eco57MI
    EcoNI EcoO109I     FalI    HaeII    Hin4I   HincII     MmeI     MscI
   MspA1I     NaeI     NcoI   NgoMIV     NspI    PflMI    PpuMI    PshAI
     PstI    PvuII    SanDI     ScaI     SfcI     SmaI     SphI     StyI
    TaqII     TatI     TstI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI     AleI
     AlfI     AloI     ApaI     AscI     AseI    AsiSI    AvrII     BaeI
    BanII     BbeI     BcgI    BciVI    BfrBI    BglII     BlpI     BmtI
    BpuEI     BsaI    BsaAI    BsaXI    BseRI    BsiEI    BsiWI     BsmI
    BspHI    BsrGI    BstBI   BstEII  BstZ17I     ClaI     DraI     DrdI
     EagI  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MslI     NarI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI     SapI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SrfI     SspI
     StuI     SwaI  Tth111I     XbaI     XcmI     XhoI     ZraI
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