##################################
# NO CUT ENZYMES (  171 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AvaII       AvrII       
BamHI       BbrPI       BbsI        BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BsiEI       BsiWI       BsmAI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstXI       BstYI       BstZ17I     
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        HapII       
HincII      HindII      HindIII     HinfI       HpaI        
HpaII       KpnI        Ksp632I     MaeI        MaeII       
MamI        MboII       MfeI        MflI        MluI        
MroI        MseI        MslI        MspI        MunI        
NaeI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsaI        RsrII       SalI        SapI        
SbfI        ScaI        SexAI       SfaNI       SfiI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TaqI        TfiI        Tru9I       
Tsp509I     TspRI       Tth111I     TthHB8I     Van91I      
XbaI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccII          56  CGCG                 CG^CG
AlwI            3  GATCC                GGATC(4/5)
AlwNI         169  CAGTTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          331  CCTGAGG              CC^T[ACGT]AGG
ApaI           41  GGGCCC               GGGCC^C
AviII         161  TGCGCA               TGC^GCA
BalI          191  TGGCCA               TGG^CCA
BanI          134  GGCGCC               G^G[CT][GA]CC
BbeI          134  GGCGCC               GGCGC^C
BbvCI         348  GCTGAGG              CCTCAGC(-5/-2)
BpmI          139  CTGGAG               CTGGAG(16/14)
BsgI          217  CTGCAC               GTGCAG(16/14)
BsrI          368  CCAGT                ACTGG(1/-1)
BstAPI         12  GCAGCCCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        327  CATCC                GGATG(2/0)
BstUI          56  CGCG                 CG^CG
Bsu36I        331  CCTGAGG              CC^T[ACGT]AGG
DpnII           3  GATC                 ^GATC
DsaI           55  CCGCGG               C^C[GA][CT]GG
EaeI          191  TGGCCA               [CT]^GGCC[GA]
Eco81I        331  CCTGAGG              CC^T[ACGT]AGG
EcoT14I        65  CCTTGG               C^C[AT][AT]GG
FokI          327  CATCC                GGATG(9/13)
FspI          161  TGCGCA               TGC^GCA
HgaI           88  GACGC                GACGC(5/10)
HphI           72  GGTGA                GGTGA(8/7)
KasI          134  GGCGCC               G^GCGCC
KspI           55  CCGCGG               CCGC^GG
MaeIII         73  GTGAC                ^GT[ACGT]AC
MboI            3  GATC                 ^GATC
MluNI         191  TGGCCA               TGG^CCA
MscI          191  TGGCCA               TGG^CCA
MvnI           56  CGCG                 CG^CG
NarI          134  GGCGCC               GG^CGCC
NdeII           3  GATC                 ^GATC
PvuII         392  CAGCTG               CAG^CTG
SacI          409  GAGCTC               GAGCT^C
SacII          55  CCGCGG               CCGC^GG
Sau3AI          3  GATC                 ^GATC
SfoI          134  GGCGCC               GGC^GCC
StyI           65  CCTTGG               C^C[AT][AT]GG
Tsp45I         73  GTGAC                ^GT[GC]AC
XcmI          128  CCAAGCGGCGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AcyI           88  GACGCC               G[GA]^CG[CT]C
              134  GGCGCC              
AspHI         123  GAGCAC               G[AT]GC[AT]^C
              409  GAGCTC              
BsaHI          88  GACGCC               G[GA]^CG[CT]C
              134  GGCGCC              
BsiHKAI       123  GAGCAC               G[AT]GC[AT]^C
              409  GAGCTC              
EcoNI         100  CCTCTCCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              138  CCTGGAGGAGG         
FauI           54  CCCGC                CCCGC(4/6)
               57  GCGGG               
Hin1I          88  GACGCC               G[GA]^CG[CT]C
              134  GGCGCC              
MspA1I         55  CCGCGG               C[AC]G^C[GT]G
              392  CAGCTG              
PstI            7  CTGCAG               CTGCA^G
              237  CTGCAG              
SfcI            7  CTGCAG               C^T[GA][CT]AG
              237  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AciI        AluI        BanII       BbvI        BmyI        
BsaJI       BseRI       BsiYI       BslI        BsmFI       
Bsp1286I    BssKI       BstNI       Cac8I       CfoI        
Cfr13I      DdeI        DraII       EcoO109I    EcoRII      
Fnu4HI      HaeII       HaeIII      HhaI        HinP1I      
ItaI        MnlI        MvaI        MwoI        NlaIII      
NlaIV       Sau96I      ScrFI       TseI