Restriction map for
pF1KB6730
(Circular) (Six-base) MAPSORT of: pF1KB6730.seq Check: 8463 from: 1 to: 3527
pF1KB6730 3527 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AccI GT'mk_AC
Cuts at: 565 565
Size: 3527
Acc65I G'GTAC_C
Cuts at: 543 543
Size: 3527
AclI AA'CG_TT
Cuts at: 3367 3367
Size: 3527
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1352 1784 2531 2799 1352
Size: 432 747 268 2080
Fragments arranged by size:
2080 747 432 268
AflIII A'CryG_T
Cuts at: 879 1983 2960 879
Size: 1104 977 1446
Fragments arranged by size:
1446 1104 977
AgeI A'CCGG_T
Cuts at: 1915 1915
Size: 3527
AlwNI CAG_nnn'CTG
Cuts at: 282 832 2399 282
Size: 550 1567 1410
Fragments arranged by size:
1567 1410 550
ApaI G_GGCC'C
Cuts at: 153 153
Size: 3527
ApaLI G'TGCA_C
Cuts at: 2297 2297
Size: 3527
ApoI r'AATT_y
Cuts at: 531 2880 3136 531
Size: 2349 256 922
Fragments arranged by size:
2349 922 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3527
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3527
AvaI C'yCGr_G
Cuts at: 547 547
Size: 3527
BamHI G'GATC_C
Cuts at: 552 552
Size: 3527
BanI G'GyrC_C
Cuts at: 242 543 957 1205 1240 242
Size: 301 414 248 35 2529
Fragments arranged by size:
2529 414 301 248 35
BanII G_rGCy'C
Cuts at: 153 394 521 541 1571 153
Size: 241 127 20 1030 2109
Fragments arranged by size:
2109 1030 241 127 20
BbeI G_GCGC'C
Cuts at: 246 961 1209 246
Size: 715 248 2564
Fragments arranged by size:
2564 715 248
BbvCI CC'TCA_GC
Cuts at: 457 457
Size: 3527
BciVI GTATCCnnnnn_n'
Cuts at: 1425 2186 3527 1425
Size: 761 1341 1425
Fragments arranged by size:
1425 1341 761
BclI T'GATC_A
Cuts at: 1047 1047
Size: 3527
BglII A'GATC_T
Cuts at: 1042 3079 1042
Size: 2037 1490
BlpI GC'TnA_GC
Cuts at: 639 639
Size: 3527
Bme1580I G_kGCm'C
Cuts at: 153 227 1152 1245 2301 153
Size: 74 925 93 1056 1379
Fragments arranged by size:
1379 1056 925 93 74
BmrI ACTGGGnnnn_n'
Cuts at: 470 739 820 1155 3299 470
Size: 269 81 335 2144 698
Fragments arranged by size:
2144 698 335 269 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1894 1894
Size: 3527
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 268
Size: 3527
Bpu10I CC'TnA_GC
Cuts at: 457 457
Size: 3527
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 703 1582 2074 2372 703
Size: 879 492 298 1858
Fragments arranged by size:
1858 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3231 3231
Size: 3527
BsaAI yAC'GTr
Cuts at: 880 880
Size: 3527
BsaBI GATnn'nnATC
Cuts at: 6 1041 3078 6
Size: 1035 2037 455
Fragments arranged by size:
2037 1035 455
BsaHI Gr'CG_yC
Cuts at: 197 243 958 1206 1908 3404 197
Size: 46 715 248 702 1496 320
Fragments arranged by size:
1496 715 702 320 248 46
BsaWI w'CCGG_w
Cuts at: 716 1237 1915 2189 2336 2747 716
Size: 521 678 274 147 411 1496
Fragments arranged by size:
1496 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 187 217 187
Size: 30 3497
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 230 265 268 304 230
Size: 35 3 36 3453
Fragments arranged by size:
3453 36 35 3
BseYI C'CCAG_C
Cuts at: 152 369 462 498 502 952 1710 2287 2783
Size: 217 93 36 4 450 758 577 496
Cuts at: 2783 152
Size: 896
Fragments arranged by size:
896 758 577 496 450 217 93 36
4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 310
Size: 3527
BsiEI CG_ry'CG
Cuts at: 104 1069 1115 2323 104
Size: 965 46 1208 1308
Fragments arranged by size:
1308 1208 965 46
BsiHKAI G_wGCw'C
Cuts at: 235 521 541 1319 1509 2301 235
Size: 286 20 778 190 792 1461
Fragments arranged by size:
1461 792 778 286 190 20
Bsp1286I G_dGCh'C
Cuts at: 153 227 235 394 521 541 1152 1245 1319
Size: 74 8 159 127 20 611 93 74
Cuts at: 1319 1509 1571 2301 153
Size: 190 62 730 1379
Fragments arranged by size:
1379 730 611 190 159 127 93 74
74 62 20 8
BspEI T'CCGG_A
Cuts at: 2747 2747
Size: 3527
BspHI T'CATG_A
Cuts at: 2703 2703
Size: 3527
BspMI ACCTGCnnnn'nnnn_
Cuts at: 577 791 1093 1474 577
Size: 214 302 381 2630
Fragments arranged by size:
2630 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1819 1873 3357 3522 50
Size: 1769 54 1484 165 55
Fragments arranged by size:
1769 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1439 1439
Size: 3527
BsrFI r'CCGG_y
Cuts at: 1525 1706 1915 1525
Size: 181 209 3137
Fragments arranged by size:
3137 209 181
BssSI C'ACGA_G
Cuts at: 1798 2156 1798
Size: 358 3169
BstAPI GCAn_nnn'nTGC
Cuts at: 126 904 126
Size: 778 2749
BstBI TT'CG_AA
Cuts at: 1888 1888
Size: 3527
BstYI r'GATC_y
Cuts at: 1 552 1019 1042 1377 1623 2635 2721 2733
Size: 551 467 23 335 246 1012 86 12
Cuts at: 2733 3079 1
Size: 346 449
Fragments arranged by size:
1012 551 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 440 440
Size: 3527
BtgI C'CryG_G
Cuts at: 163 163
Size: 3527
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1477 1658 1821 1477
Size: 181 163 3183
Fragments arranged by size:
3183 181 163
BtsI GCAGTG_nn'
Cuts at: 770 770
Size: 3527
DraI TTT'AAA
Cuts at: 526 526
Size: 3527
DrdI GACnn_nn'nnGTC
Cuts at: 1233 2091 1233
Size: 858 2669
EaeI y'GGCC_r
Cuts at: 299 1112 1286 1677 1704 3049 299
Size: 813 174 391 27 1345 777
Fragments arranged by size:
1345 813 777 391 174 27
EagI C'GGCC_G
Cuts at: 1112 1112
Size: 3527
EarI CTCTTCn'nnn_
Cuts at: 1550 1760 1550
Size: 210 3317
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1735 2045 2191 3337 1735
Size: 310 146 1146 1925
Fragments arranged by size:
1925 1146 310 146
EcoICRI GAG'CTC
Cuts at: 519 539 519
Size: 20 3507
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 1352 1784 2531 2799 268
Size: 1084 432 747 268 996
Fragments arranged by size:
1084 996 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 212 250 212
Size: 38 3489
EcoO109I rG'GnC_Cy
Cuts at: 136 149 189 444 666 136
Size: 13 40 255 222 2997
Fragments arranged by size:
2997 255 222 40 13
EcoRI G'AATT_C
Cuts at: 531 531
Size: 3527
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1546 1546
Size: 3527
FspI TGC'GCA
Cuts at: 271 1308 271
Size: 1037 2490
HaeII r_GCGC'y
Cuts at: 159 246 420 961 1209 2231 3210 159
Size: 87 174 541 248 1022 979 476
Fragments arranged by size:
1022 979 541 476 248 174 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 187 219 1369 1399 1401 1431 187
Size: 32 1150 30 2 30 2283
Fragments arranged by size:
2283 1150 32 30 30 2
HincII GTy'rAC
Cuts at: 566 566
Size: 3527
KasI G'GCGC_C
Cuts at: 242 957 1205 242
Size: 715 248 2564
Fragments arranged by size:
2564 715 248
KpnI G_GTAC'C
Cuts at: 547 547
Size: 3527
MluI A'CGCG_T
Cuts at: 2960 2960
Size: 3527
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2198 2382 2198
Size: 184 3343
MscI TGG'CCA
Cuts at: 301 1288 301
Size: 987 2540
MslI CAynn'nnrTG
Cuts at: 1516 1643 1516
Size: 127 3400
MspA1I CmG'CkG
Cuts at: 165 502 638 952 1312 2325 2570 165
Size: 337 136 314 360 1013 245 1122
Fragments arranged by size:
1122 1013 360 337 314 245 136
NaeI GCC'GGC
Cuts at: 1708 1708
Size: 3527
NarI GG'CG_CC
Cuts at: 243 958 1206 243
Size: 715 248 2564
Fragments arranged by size:
2564 715 248
NgoMIV G'CCGG_C
Cuts at: 1706 1706
Size: 3527
NspI r_CATG'y
Cuts at: 580 1080 1987 580
Size: 500 907 2120
Fragments arranged by size:
2120 907 500
PasI CC'CwG_GG
Cuts at: 141 141
Size: 3527
PciI A'CATG_T
Cuts at: 1983 1983
Size: 3527
PmeI GTTT'AAAC
Cuts at: 526 526
Size: 3527
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2666 2698 2666
Size: 32 3495
PspOMI G'GGCC_C
Cuts at: 149 149
Size: 3527
PstI C_TGCA'G
Cuts at: 119 349 574 1259 119
Size: 230 225 685 2387
Fragments arranged by size:
2387 685 230 225
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3527
PvuII CAG'CTG
Cuts at: 502 952 1312 502
Size: 450 360 2717
Fragments arranged by size:
2717 450 360
RsrII CG'GwC_CG
Cuts at: 1722 1722
Size: 3527
SacI G_AGCT'C
Cuts at: 521 541 521
Size: 20 3507
SacII CC_GC'GG
Cuts at: 166 166
Size: 3527
SalI G'TCGA_C
Cuts at: 564 564
Size: 3527
SapI GCTCTTCn'nnn_
Cuts at: 1550 1760 1550
Size: 210 3317
SbfI CC_TGCA'GG
Cuts at: 574 574
Size: 3527
ScaI AGT'ACT
Cuts at: 3044 3044
Size: 3527
SfcI C'TryA_G
Cuts at: 33 115 345 570 1255 2248 2439 33
Size: 82 230 225 685 993 191 1121
Fragments arranged by size:
1121 993 685 230 225 191 82
SfoI GGC'GCC
Cuts at: 244 959 1207 244
Size: 715 248 2564
Fragments arranged by size:
2564 715 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3527
SmaI CCC'GGG
Cuts at: 549 549
Size: 3527
SmlI C'TyrA_G
Cuts at: 682 1597 2089 2351 682
Size: 915 492 262 1858
Fragments arranged by size:
1858 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3527
SphI G_CATG'C
Cuts at: 580 1080 580
Size: 500 3027
StyI C'CwwG_G
Cuts at: 173 661 1015 173
Size: 488 354 2685
Fragments arranged by size:
2685 488 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 319 1110 1243 1702 1911 319
Size: 791 133 459 209 1935
Fragments arranged by size:
1935 791 459 209 133
TatI w'GTAC_w
Cuts at: 3042 3042
Size: 3527
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 462 494 3044 3076 462
Size: 32 2550 32 913
Fragments arranged by size:
2550 913 32 32
Tth111I GACn'n_nGTC
Cuts at: 1324 1324
Size: 3527
XbaI T'CTAG_A
Cuts at: 558 558
Size: 3527
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 243 1022 243
Size: 779 2748
XmaI C'CCGG_G
Cuts at: 547 547
Size: 3527
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BcgI BfrBI BglI BmgBI
BmtI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PflMI PfoI PmlI PpuMI PshAI PsiI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI