Restriction map for pF1KB6212
##################################
# NO CUT ENZYMES (   81 enzymes) #
##################################

AclI        AflII       AgeI        AhdI        Alw44I      
ApaLI       AscI        AseI        AsnI        Asp700I     
AspEI       AvrII       BamHI       BcgI        BclI        
BfrI        BglI        BglII       BlnI        BsaI        
BsiWI       BsmI        BspDI       BspHI       BsrDI       
BssHII      Bst1107I    BstBI       BstYI       BstZ17I     
ClaI        CpoI        DraI        DraIII      EagI        
Eam1105I    EclXI       Eco52I      EcoRV       EcoT22I     
FbaI        FseI        HpaI        KspI        MfeI        
MflI        MluI        MseI        MunI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmeI        PshAI       
PshBI       Psp1406I    RcaI        RsrII       SacII       
SalI        SapI        ScaI        SfiI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        SspI        
SwaI        Tru9I       XbaI        XhoI        XhoII       
XmnI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

AatII        1018  GACGTC               GACGT^C
AccI          241  GTCTAC               GT^[AC][GT]AC
AccIII        891  TCCGGA               T^CCGGA
AcsI            6  GAATTC               [GA]^AATT[CT]
AocI         1242  CCTCAGG              CC^T[ACGT]AGG
Aor51HI      1816  AGCGCT               AGC^GCT
ApoI            6  GAATTC               [GA]^AATT[CT]
AviII        1811  TGCGCA               TGC^GCA
BalI          788  TGGCCA               TGG^CCA
BbeI         1189  GGCGCC               GGCGC^C
BbrPI         196  CACGTG               CAC^GTG
BbvCI        1311  GCTGAGG              CCTCAGC(-5/-2)
BciVI         201  GTATCC               GTATCC(6/5)
BfaI          622  CTAG                 C^TAG
BlpI         1536  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1536  GCTCAGC              GC^T[ACGT]AGC
BsaBI        1293  GATTCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI         891  TCCGGA               T^CCGGA
BsiEI        1919  CGATCG               CG[GA][CT]^CG
Bsp1407I     1040  TGTACA               T^GTACA
BspEI         891  TCCGGA               T^CCGGA
BspLU11I     1868  ACATGT               A^CATGT
BsrGI        1040  TGTACA               T^GTACA
BssSI        1584  CTCGTG               CACGAG(-5/-1)
Bsu36I       1242  CCTCAGG              CC^T[ACGT]AGG
CelII        1536  GCTCAGC              GC^T[ACGT]AGC
DrdI         1690  GACAGTGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1160  CTCTTC               CTCTTC(1/4)
Eco47III     1816  AGCGCT               AGC^GCT
Eco81I       1242  CCTCAGG              CC^T[ACGT]AGG
EcoRI           6  GAATTC               G^AATTC
FspI         1811  TGCGCA               TGC^GCA
HincII       1377  GTTGAC               GT[CT]^[GA]AC
HindII       1377  GTTGAC               GT[CT]^[GA]AC
HindIII      1210  AAGCTT               A^AGCTT
KasI         1189  GGCGCC               G^GCGCC
Ksp632I      1160  CTCTTC               CTCTTC(1/4)
MaeI          622  CTAG                 C^TAG
MamI         1293  GATTCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         788  TGGCCA               TGG^CCA
MroI          891  TCCGGA               T^CCGGA
MscI          788  TGGCCA               TGG^CCA
NaeI         1723  GCCGGC               GCC^GGC
NarI         1189  GGCGCC               GG^CGCC
NgoMIV       1723  GCCGGC               G^CCGGC
PmaCI         196  CACGTG               CAC^GTG
PmlI          196  CACGTG               CAC^GTG
PvuI         1919  CGATCG               CGAT^CG
SacI         1659  GAGCTC               GAGCT^C
SbfI         2082  CCTGCAGG             CCTGCA^GG
SexAI        1877  ACCTGGT              A^CC[AT]GGT
SfoI         1189  GGCGCC               GGC^GCC
SmaI         1496  CCCGGG               CCC^GGG
SphI         1719  GCATGC               GCATG^C
Sse8387I     2082  CCTGCAGG             CCTGCA^GG
SspBI        1040  TGTACA               T^GTACA
XcmI          479  CCAGACACTCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        1496  CCCGGG               C^CCGGG
XmaI         1496  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

Acc65I        168  GGTACC               G^GTACC
              824  GGTACC              
AlwI          657  GATCC                GGATC(4/5)
             1794  GATCC               
ApaI          128  GGGCCC               GGGCC^C
             2016  GGGCCC              
Asp718I       168  GGTACC               G^GTACC
              824  GGTACC              
AspI          235  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1000  GACGGCGTC           
BsaAI         196  CACGTG               [CT]AC^GT[GA]
              220  TACGTG              
BsaWI         891  TCCGGA               [AT]^CCGG[AT]
             1906  ACCGGA              
BspMI         165  GCAGGT               ACCTGC(4/8)
              177  GCAGGT              
BsrBI         504  CCGCTC               CCGCTC(-3/-3)
               20  GAGCGG              
BstEII        348  GGTGACC              G^GT[ACGT]ACC
             1698  GGTCACC             
BstPI         348  GGTGACC              G^GT[ACGT]ACC
             1698  GGTCACC             
EcoO65I       348  GGTGACC              G^GT[ACGT]ACC
             1698  GGTCACC             
KpnI          168  GGTACC               GGTAC^C
              824  GGTACC              
NspI         1719  GCATGC               [GA]CATG^[CT]
             1868  ACATGT              
PflFI         235  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1000  GACGGCGTC           
PflMI         387  CCACAGCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1278  CCAGGCGCTGG         
PleI          748  GACTC                GAGTC(4/5)
              564  GAGTC               
PpuMI        1706  AGGTCCT              [GA]G^G[AT]CC[CT]
             1799  GGGACCT             
PvuII        1033  CAGCTG               CAG^CTG
             1680  CAGCTG              
StuI         1133  AGGCCT               AGG^CCT
             1580  AGGCCT              
Tsp509I         7  AATT                 ^AATT
              541  AATT                
Tth111I       235  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1000  GACGGCGTC           
Van91I        387  CCACAGCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1278  CCAGGCGCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   95 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AlwNI       AspHI       AvaI        AvaII       
BanI        BanII       BbsI        BbvI        BcnI        
BmyI        BpmI        BpuAI       BsaHI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BsrFI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstUI       BstXI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeII       MaeIII      MboI        MboII       MnlI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TaqI        
TfiI        TseI        Tsp45I      TspRI       TthHB8I     
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