Restriction map for pF1KB6212
 (Linear) (Six-base) MAPSORT of: KIBB6212.seq  Check: 8649  from: 1  to: 2094



KIBB6212.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     168    2094 
   Size:        168    1926
 
AatII G_ACGT'C

Cuts at:      0    1022    2094 
   Size:       1022    1072
 
AccI GT'mk_AC

Cuts at:      0     242    2094 
   Size:        242    1852
 
Acc65I G'GTAC_C

Cuts at:      0     168     824    2094 
   Size:        168     656    1270

  Fragments arranged by size: 

                1270     656     168
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     760    1732    2094 
   Size:        760     972     362

  Fragments arranged by size: 

                 972     760     362
 
AfeI AGC'GCT

Cuts at:      0    1818    2094 
   Size:       1818     276
 
AflIII A'CryG_T

Cuts at:      0     197    1466    1514    1868    2094 
   Size:        197    1269      48     354     226

  Fragments arranged by size: 

                1269     354     226     197      48
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1006    2094 
   Size:       1006    1088
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     625     657    2094 
   Size:        625      32    1437

  Fragments arranged by size: 

                1437     625      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     531    1284    1455    1623    1710    2094 
   Size:        531     753     171     168      87     384

  Fragments arranged by size: 

                 753     531     384     171     168      87
 
ApaI G_GGCC'C

Cuts at:      0     132    2020    2094 
   Size:        132    1888      74

  Fragments arranged by size: 

                1888     132      74
 
ApoI r'AATT_y

Cuts at:      0       6    2094 
   Size:          6    2088
 
AvaI C'yCGr_G

Cuts at:      0     283     853     958    1183    1496    2094 
   Size:        283     570     105     225     313     598

  Fragments arranged by size: 

                 598     570     313     283     225     105
 
BanI G'GyrC_C

Cuts at:      0     168     340     697     824    1189    2094 
   Size:        168     172     357     127     365     905

  Fragments arranged by size: 

                 905     365     357     172     168     127
 
BanII G_rGCy'C

Cuts at:      0     132     427    1663    2020    2045    2094 
   Size:        132     295    1236     357      25      49

  Fragments arranged by size: 

                1236     357     295     132      49      25
 
BbeI G_GCGC'C

Cuts at:      0    1193    2094 
   Size:       1193     901
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     847     945    1184    2094 
   Size:        847      98     239     910

  Fragments arranged by size: 

                 910     847     239      98
 
BbvCI CC'TCA_GC

Cuts at:      0    1312    2094 
   Size:       1312     782
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     212    2094 
   Size:        212    1882
 
BlpI GC'TnA_GC

Cuts at:      0    1537    2094 
   Size:       1537     557
 
Bme1580I G_kGCm'C

Cuts at:      0     132     345     700    2020    2094 
   Size:        132     213     355    1320      74

  Fragments arranged by size: 

                1320     355     213     132      74
 
BmgBI CAC'GTC

Cuts at:      0     951    1308    1469    1517    2094 
   Size:        951     357     161      48     577

  Fragments arranged by size: 

                 951     577     357     161      48
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     622    1319    2094 
   Size:        622     697     775

  Fragments arranged by size: 

                 775     697     622
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     469    1599    2094 
   Size:        469    1130     495

  Fragments arranged by size: 

                1130     495     469
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     420     508    1069    1125    1645    2094 
   Size:        420      88     561      56     520     449

  Fragments arranged by size: 

                 561     520     449     420      88      56
 
Bpu10I CC'TnA_GC

Cuts at:      0    1312    2094 
   Size:       1312     782
 
BsaAI yAC'GTr

Cuts at:      0     198     222    2094 
   Size:        198      24    1872

  Fragments arranged by size: 

                1872     198      24
 
BsaBI GATnn'nnATC

Cuts at:      0    1297    2094 
   Size:       1297     797
 
BsaHI Gr'CG_yC

Cuts at:      0     236    1004    1019    1190    1430    1915    2024    2094 
   Size:        236     768      15     171     240     485     109      70

  Fragments arranged by size: 

                 768     485     240     236     171     109      70      15
            
 
BsaWI w'CCGG_w

Cuts at:      0     891    1906    2094 
   Size:        891    1015     188

  Fragments arranged by size: 

                1015     891     188
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     224     254     625     655     850     880    2094 
   Size:        224      30     371      30     195      30    1214

  Fragments arranged by size: 

                1214     371     224     195      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1128    1269    1618    2094 
   Size:       1128     141     349     476

  Fragments arranged by size: 

                1128     476     349     141
 
BseYI C'CCAG_C

Cuts at:      0     173     304     693     966    1732    2094 
   Size:        173     131     389     273     766     362

  Fragments arranged by size: 

                 766     389     362     273     173     131
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202     211     235     865     931     997    1591    1636 
   Size:        202       9      24     630      66      66     594      45

Cuts at:   1636    2094 
   Size:        458

  Fragments arranged by size: 

                 630     594     458     202      66      66      45      24
                   9
 
BsiEI CG_ry'CG

Cuts at:      0    1922    2094 
   Size:       1922     172
 
BsiHKAI G_wGCw'C

Cuts at:      0     617    1663    1889    2094 
   Size:        617    1046     226     205

  Fragments arranged by size: 

                1046     617     226     205
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     227     438    1906    2094 
   Size:        227     211    1468     188

  Fragments arranged by size: 

                1468     227     211     188
 
Bsp1286I G_dGCh'C

Cuts at:      0     132     345     427     617     700    1663    1889    2020 
   Size:        132     213      82     190      83     963     226     131

Cuts at:   2020    2045    2094 
   Size:         25      49

  Fragments arranged by size: 

                 963     226     213     190     132     131      83      82
                  49      25
 
BspEI T'CCGG_A

Cuts at:      0     891    2094 
   Size:        891    1203
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     156     168    2094 
   Size:        156      12    1926

  Fragments arranged by size: 

                1926     156      12
 
BsrBI CCG'CTC

Cuts at:      0      22     506    2094 
   Size:         22     484    1588

  Fragments arranged by size: 

                1588     484      22
 
BsrFI r'CCGG_y

Cuts at:      0     186     761     827    1317    1723    2094 
   Size:        186     575      66     490     406     371

  Fragments arranged by size: 

                 575     490     406     371     186      66
 
BsrGI T'GTAC_A

Cuts at:      0    1040    2094 
   Size:       1040    1054
 
BssSI C'ACGA_G

Cuts at:      0    1584    2094 
   Size:       1584     510
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     189     807    1761    2094 
   Size:        189     618     954     333

  Fragments arranged by size: 

                 954     618     333     189
 
BstEII G'GTnAC_C

Cuts at:      0     348    1698    2094 
   Size:        348    1350     396

  Fragments arranged by size: 

                1350     396     348
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     727    1365    1686    2094 
   Size:        727     638     321     408

  Fragments arranged by size: 

                 727     638     408     321
 
Bsu36I CC'TnA_GG

Cuts at:      0    1243    2094 
   Size:       1243     851
 
BtgI C'CryG_G

Cuts at:      0      38     353     407     459     902    1787    2094 
   Size:         38     315      54      52     443     885     307

  Fragments arranged by size: 

                 885     443     315     307      54      52      38
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     494    2006    2094 
   Size:        494    1512      88

  Fragments arranged by size: 

                1512     494      88
 
BtsI GCAGTG_nn'

Cuts at:      0     603    1009    1050    2070    2094 
   Size:        603     406      41    1020      24

  Fragments arranged by size: 

                1020     603     406      41      24
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1696    2094 
   Size:       1696     398
 
EaeI y'GGCC_r

Cuts at:      0     788     829     905    2094 
   Size:        788      41      76    1189

  Fragments arranged by size: 

                1189     788      76      41
 
EarI CTCTTCn'nnn_

Cuts at:      0    1166    2094 
   Size:       1166     928
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1837    2094 
   Size:       1837     257
 
EcoICRI GAG'CTC

Cuts at:      0    1661    2094 
   Size:       1661     433
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     420     508     760    1069    1125    1645    1732    2094 
   Size:        420      88     252     309      56     520      87     362

  Fragments arranged by size: 

                 520     420     362     309     252      88      87      56
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     994    1201    1276    1486    2094 
   Size:        994     207      75     210     608

  Fragments arranged by size: 

                 994     608     210     207      75
 
EcoO109I rG'GnC_Cy

Cuts at:      0     128     330    1026    1707    1800    2094 
   Size:        128     202     696     681      93     294

  Fragments arranged by size: 

                 696     681     294     202     128      93
 
EcoRI G'AATT_C

Cuts at:      0       6    2094 
   Size:          6    2088
 
FspI TGC'GCA

Cuts at:      0    1813    2094 
   Size:       1813     281
 
HaeII r_GCGC'y

Cuts at:      0     973    1193    1285    1464    1820    2094 
   Size:        973     220      92     179     356     274

  Fragments arranged by size: 

                 973     356     274     220     179      92
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     224     256     850     882    1936    1968    2094 
   Size:        224      32     594      32    1054      32     126

  Fragments arranged by size: 

                1054     594     224     126      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1379    2094 
   Size:       1379     715
 
HindIII A'AGCT_T

Cuts at:      0    1210    2094 
   Size:       1210     884
 
KasI G'GCGC_C

Cuts at:      0    1189    2094 
   Size:       1189     905
 
KpnI G_GTAC'C

Cuts at:      0     172     828    2094 
   Size:        172     656    1266

  Fragments arranged by size: 

                1266     656     172
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     515    2094 
   Size:        515    1579
 
MscI TGG'CCA

Cuts at:      0     790    2094 
   Size:        790    1304
 
MslI CAynn'nnrTG

Cuts at:      0     406     725     790     907    2094 
   Size:        406     319      65     117    1187

  Fragments arranged by size: 

                1187     406     319     117      65
 
MspA1I CmG'CkG

Cuts at:      0     376     553     863     875    1035    1620    1682    2094 
   Size:        376     177     310      12     160     585      62     412

  Fragments arranged by size: 

                 585     412     376     310     177     160      62      12
            
 
NaeI GCC'GGC

Cuts at:      0    1725    2094 
   Size:       1725     369
 
NarI GG'CG_CC

Cuts at:      0    1190    2094 
   Size:       1190     904
 
NcoI C'CATG_G

Cuts at:      0     407     902    1787    2094 
   Size:        407     495     885     307

  Fragments arranged by size: 

                 885     495     407     307
 
NgoMIV G'CCGG_C

Cuts at:      0    1723    2094 
   Size:       1723     371
 
NspI r_CATG'y

Cuts at:      0    1723    1872    2094 
   Size:       1723     149     222

  Fragments arranged by size: 

                1723     222     149
 
PciI A'CATG_T

Cuts at:      0    1868    2094 
   Size:       1868     226
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     393    1284    2094 
   Size:        393     891     810

  Fragments arranged by size: 

                 891     810     393
 
PfoI T'CCnGG_A

Cuts at:      0    1796    2094 
   Size:       1796     298
 
PmlI CAC'GTG

Cuts at:      0     198    2094 
   Size:        198    1896
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     625     657    1855    1887    2094 
   Size:        625      32    1198      32     207

  Fragments arranged by size: 

                1198     625     207      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1707    1800    2094 
   Size:       1707      93     294

  Fragments arranged by size: 

                1707     294      93
 
PspOMI G'GGCC_C

Cuts at:      0     128    2016    2094 
   Size:        128    1888      78

  Fragments arranged by size: 

                1888     128      78
 
PstI C_TGCA'G

Cuts at:      0     770     998    1034    1511    1652    2087    2094 
   Size:        770     228      36     477     141     435       7

  Fragments arranged by size: 

                 770     477     435     228     141      36       7
 
PvuI CG_AT'CG

Cuts at:      0    1922    2094 
   Size:       1922     172
 
PvuII CAG'CTG

Cuts at:      0    1035    1682    2094 
   Size:       1035     647     412

  Fragments arranged by size: 

                1035     647     412
 
SacI G_AGCT'C

Cuts at:      0    1663    2094 
   Size:       1663     431
 
SbfI CC_TGCA'GG

Cuts at:      0    2087    2094 
   Size:       2087       7
 
SexAI A'CCwGG_T

Cuts at:      0    1877    2094 
   Size:       1877     217
 
SfcI C'TryA_G

Cuts at:      0     766     994    1030    1064    1507    1648    2083    2094 
   Size:        766     228      36      34     443     141     435      11

  Fragments arranged by size: 

                 766     443     435     228     141      36      34      11
            
 
SfoI GGC'GCC

Cuts at:      0    1191    2094 
   Size:       1191     903
 
SmaI CCC'GGG

Cuts at:      0    1498    2094 
   Size:       1498     596
 
SphI G_CATG'C

Cuts at:      0    1723    2094 
   Size:       1723     371
 
SrfI GCCC'GGGC

Cuts at:      0    1498    2094 
   Size:       1498     596
 
StuI AGG'CCT

Cuts at:      0    1135    1582    2094 
   Size:       1135     447     512

  Fragments arranged by size: 

                1135     512     447
 
StyI C'CwwG_G

Cuts at:      0     407     902    1202    1421    1742    1787    2094 
   Size:        407     495     300     219     321      45     307

  Fragments arranged by size: 

                 495     407     321     307     300     219      45
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1578    1717    2026    2094 
   Size:       1578     139     309      68

  Fragments arranged by size: 

                1578     309     139      68
 
TatI w'GTAC_w

Cuts at:      0     782     932    1040    1865    2094 
   Size:        782     150     108     825     229

  Fragments arranged by size: 

                 825     782     229     150     108
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1146    1178    1328    1360    2094 
   Size:       1146      32     150      32     734

  Fragments arranged by size: 

                1146     734     150      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     238    1003    2094 
   Size:        238     765    1091

  Fragments arranged by size: 

                1091     765     238
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     486    2094 
   Size:        486    1608
 
XmaI C'CCGG_G

Cuts at:      0    1496    2094 
   Size:       1496     598
 
ZraI GAC'GTC

Cuts at:      0    1020    2094 
   Size:       1020    1074
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI     AfeI   AflIII     AlfI
     AloI    AlwNI     ApaI     ApoI     AvaI     BanI    BanII     BbeI
     BbsI    BbvCI    BciVI     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI
    BsrBI    BsrFI    BsrGI    BssSI   BstAPI   BstEII    BstXI   Bsu36I
     BtgI    BtgZI     BtsI     DrdI     EaeI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII
  HindIII     KasI     KpnI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PciI    PflMI     PfoI     PmlI
     PpiI    PpuMI   PspOMI     PstI     PvuI    PvuII     SacI     SbfI
    SexAI     SfcI     SfoI     SmaI     SphI     SrfI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AclI    AflII     AgeI     AhdI     AleI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BcgI     BclI    BfrBI     BglI
    BglII     BmtI    BpuEI     BsaI    BsiWI     BsmI    BspHI    BsrDI
   BssHII    BstBI    BstYI  BstZ17I     ClaI    CspCI     DraI   DraIII
     EagI    EcoRV     FalI     FseI    FspAI     HpaI     MfeI     MluI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI     PmeI
    PshAI     PsiI    PspXI     PsrI    RsrII    SacII     SalI    SanDI
     SapI     ScaI     SfiI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SspI     SwaI     XbaI     XhoI     XmnI
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