Restriction map for
pF1KB6208
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
AccI AclI AhdI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BamHI BbrPI BbsI BbvCI
BcgI BciVI BglII BlnI BpuAI
BsaAI BsaBI BsiEI BsiWI BspDI
BspHI BspLU11I BsrBI BssHII Bst1107I
BstAPI BstBI BstZ17I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco52I
EcoNI EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
KspI MamI MfeI MluI MunI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI ScaI SfiI SfuI SgrAI
SnaBI SpeI SphI SspI SwaI
TaqI TfiI Tth111I TthHB8I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 124 GACGTC GACGT^C
Acc65I 1055 GGTACC G^GTACC
AcsI 731 GAATTT [GA]^AATT[CT]
AflII 709 CTTAAG C^TTAAG
AflIII 356 ACGTGT A^C[GA][CT]GT
AgeI 1058 ACCGGT A^CCGGT
Alw44I 1120 GTGCAC G^TGCAC
Aor51HI 212 AGCGCT AGC^GCT
ApaI 528 GGGCCC GGGCC^C
ApaLI 1120 GTGCAC G^TGCAC
ApoI 731 GAATTT [GA]^AATT[CT]
Asp718I 1055 GGTACC G^GTACC
BclI 939 TGATCA T^GATCA
BfaI 389 CTAG C^TAG
BfrI 709 CTTAAG C^TTAAG
BglI 1013 GCCCTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1960 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1960 GCTGAGC GC^T[ACGT]AGC
BsgI 1714 CTGCAC GTGCAG(16/14)
BsmBI 353 GAGACG CGTCTC(1/5)
Bsp1407I 600 TGTACA T^GTACA
BspMI 1529 ACCTGC ACCTGC(4/8)
BsrDI 761 CATTGC GCAATG(2/0)
BsrGI 600 TGTACA T^GTACA
BssSI 1705 CACGAG CACGAG(-5/-1)
BstEII 2025 GGTCACC G^GT[ACGT]ACC
BstPI 2025 GGTCACC G^GT[ACGT]ACC
BstYI 651 AGATCC [GA]^GATC[CT]
CelII 1960 GCTGAGC GC^T[ACGT]AGC
DraI 159 TTTAAA TTT^AAA
EarI 274 GAAGAG CTCTTC(1/4)
Eco47III 212 AGCGCT AGC^GCT
EcoO65I 2025 GGTCACC G^GT[ACGT]ACC
FbaI 939 TGATCA T^GATCA
HgaI 1563 GCGTC GACGC(5/10)
KpnI 1055 GGTACC GGTAC^C
Ksp632I 274 GAAGAG CTCTTC(1/4)
MaeI 389 CTAG C^TAG
MflI 651 AGATCC [GA]^GATC[CT]
NaeI 1343 GCCGGC GCC^GGC
NcoI 346 CCATGG C^CATGG
NgoMIV 1343 GCCGGC G^CCGGC
PinAI 1058 ACCGGT A^CCGGT
SacI 1700 GAGCTC GAGCT^C
SbfI 1953 CCTGCAGG CCTGCA^GG
SmaI 173 CCCGGG CCC^GGG
SmlI 709 CTTAAG C^T[CT][GA]AG
Sse8387I 1953 CCTGCAGG CCTGCA^GG
SspBI 600 TGTACA T^GTACA
XhoII 651 AGATCC [GA]^GATC[CT]
XmaCI 173 CCCGGG C^CCGGG
XmaI 173 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccIII 94 TCCGGA T^CCGGA
1720 TCCGGA
AlwI 652 GATCC GGATC(4/5)
443 GGATC
AlwNI 488 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
968 CAGAGCCTG
AocI 1863 CCTCAGG CC^T[ACGT]AGG
1891 CCTGAGG
BpmI 412 CTGGAG CTGGAG(16/14)
974 CTGGAG
BsaI 1265 GAGACC GGTCTC(1/5)
1682 GAGACC
BseAI 94 TCCGGA T^CCGGA
1720 TCCGGA
BsmI 790 GAATGC GAATGC(1/-1)
1081 GCATTC
BspEI 94 TCCGGA T^CCGGA
1720 TCCGGA
Bsu36I 1863 CCTCAGG CC^T[ACGT]AGG
1891 CCTGAGG
Eco81I 1863 CCTCAGG CC^T[ACGT]AGG
1891 CCTGAGG
HinfI 280 GAGTC G^A[ACGT]TC
592 GAGTC
MroI 94 TCCGGA T^CCGGA
1720 TCCGGA
MseI 160 TTAA T^TAA
710 TTAA
MslI 1291 CATTCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1941 CACTGTGGTG
NdeI 1195 CATATG CA^TATG
1456 CATATG
PflMI 967 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1194 CCATATGCTGG
PleI 280 GAGTC GAGTC(4/5)
592 GAGTC
PpuMI 236 GGGACCC [GA]G^G[AT]CC[CT]
848 AGGACCC
SexAI 1280 ACCAGGT A^CC[AT]GGT
1376 ACCAGGT
StuI 1247 AGGCCT AGG^CCT
1331 AGGCCT
Tru9I 160 TTAA T^TAA
710 TTAA
Tsp509I 732 AATT ^AATT
832 AATT
Van91I 967 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1194 CCATATGCTGG
XcmI 663 CCAGTCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1829 CCACCTGTGGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbvI BcnI BmyI
BsaHI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HpaII HphI ItaI KasI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
StyI TseI Tsp45I TspRI