Restriction map for pF1KB6189
##################################
# NO CUT ENZYMES (  106 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        Aor51HI     
ApaI        AscI        Asp700I     Asp718I     AviII       
AvrII       BamHI       BanI        BanII       BbeI        
BbrPI       BbvCI       BciVI       BclI        BcnI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaI        BseAI       BsiEI       
BsiWI       Bsp1407I    BspDI       BspEI       BsrBI       
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstEII      BstPI       BstXI       BstZ17I     CelII       
ClaI        CpoI        DraIII      DrdI        EagI        
EclXI       Eco47III    Eco52I      EcoO65I     EcoRI       
FbaI        FseI        FspI        HaeII       HgaI        
HpaI        KasI        KpnI        KspI        MamI        
MluI        MroI        NaeI        NarI        NciI        
NdeI        NgoMIV      NheI        NotI        NruI        
PacI        PflMI       PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       StuI        
SwaI        Van91I      XbaI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AatII         979  GACGTC               GACGT^C
AcyI          979  GACGTC               G[GA]^CG[CT]C
AfaI            7  GTAC                 GT^AC
AflII         495  CTTAAG               C^TTAAG
AhdI          306  GACACCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1240  GTGCAC               G^TGCAC
AlwNI        1919  CAGTAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          633  CCTGAGG              CC^T[ACGT]AGG
ApaLI        1240  GTGCAC               G^TGCAC
AseI          484  ATTAAT               AT^TAAT
AsnI          484  ATTAAT               AT^TAAT
AspEI         306  GACACCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1447  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BcgI          737  GCATGCTCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI          495  CTTAAG               C^TTAAG
BpmI          889  CTCCAG               CTGGAG(16/14)
BsaHI         979  GACGTC               G[GA]^CG[CT]C
BsgI         1063  GTGCAG               GTGCAG(16/14)
BsmBI         981  CGTCTC               CGTCTC(1/5)
BsmI         1473  GAATGC               GAATGC(1/-1)
BspHI        1701  TCATGA               T^CATGA
BspLU11I     1892  ACATGT               A^CATGT
BstAPI       1918  GCAGTAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         173  TTCGAA               TT^CGAA
Bsu36I        633  CCTGAGG              CC^T[ACGT]AGG
DraII         724  GGGACCC              [GA]G^G[ACGT]CC[CT]
DsaI         1418  CCATGG               C^C[GA][CT]GG
Eam1105I      306  GACACCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        633  CCTGAGG              CC^T[ACGT]AGG
EcoNI         237  CCTACAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      724  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoRV        1948  GATATC               GAT^ATC
FauI          944  CCCGC                CCCGC(4/6)
Hin1I         979  GACGTC               G[GA]^CG[CT]C
MfeI          520  CAATTG               C^AATTG
MslI          869  CATATCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I       1589  CAGCTG               C[AC]G^C[GT]G
MunI          520  CAATTG               C^AATTG
NcoI         1418  CCATGG               C^CATGG
NspV          173  TTCGAA               TT^CGAA
PflFI        1447  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         724  GGGACCC              [GA]G^G[AT]CC[CT]
PshBI         484  ATTAAT               AT^TAAT
PvuII        1589  CAGCTG               CAG^CTG
RcaI         1701  TCATGA               T^CATGA
RsaI            7  GTAC                 GT^AC
SfuI          173  TTCGAA               TT^CGAA
SphI          737  GCATGC               GCATG^C
Tsp45I        304  GTGAC                ^GT[GC]AC
Tth111I      1447  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   37 enzymes) #
###################################

AccII          27  CGCG                 CG^CG
               29  CGCG                
AflIII       1360  ACACGT               A^C[GA][CT]GT
             1892  ACATGT              
AgeI           80  ACCGGT               A^CCGGT
             1532  ACCGGT              
AvaI         1301  CTCGAG               C^[CT]CG[GA]G
             1676  CTCGAG              
AvaII         472  GGTCC                G^G[AT]CC
              725  GGACC               
BalI         1640  TGGCCA               TGG^CCA
             1934  TGGCCA              
BsaWI          80  ACCGGT               [AT]^CCGG[AT]
             1532  ACCGGT              
BseRI         293  CTCCTC               GAGGAG(10/8)
              847  GAGGAG              
BsoBI        1301  CTCGAG               C^[CT]CG[GA]G
             1676  CTCGAG              
BstUI          27  CGCG                 CG^CG
               29  CGCG                
BstYI         801  GGATCT               [GA]^GATC[CT]
             1389  GGATCT              
CfoI           28  GCGC                 GCG^C
             1380  GCGC                
Cfr13I        472  GGTCC                G^G[ACGT]CC
              725  GGACC               
DraI          144  TTTAAA               TTT^AAA
             1354  TTTAAA              
EaeI         1640  TGGCCA               [CT]^GGCC[GA]
             1934  TGGCCA              
EarI           49  GAAGAG               CTCTTC(1/4)
              831  GAAGAG              
EcoT22I       619  ATGCAT               ATGCA^T
              971  ATGCAT              
HhaI           28  GCGC                 GCG^C
             1380  GCGC                
HinP1I         28  GCGC                 G^CGC
             1380  GCGC                
HincII        586  GTTGAC               GT[CT]^[GA]AC
              639  GTCAAC              
HindII        586  GTTGAC               GT[CT]^[GA]AC
              639  GTCAAC              
HindIII       917  AAGCTT               A^AGCTT
              925  AAGCTT              
Ksp632I        49  GAAGAG               CTCTTC(1/4)
              831  GAAGAG              
MflI          801  GGATCT               [GA]^GATC[CT]
             1389  GGATCT              
MluNI        1640  TGGCCA               TGG^CCA
             1934  TGGCCA              
MscI         1640  TGGCCA               TGG^CCA
             1934  TGGCCA              
MvnI           27  CGCG                 CG^CG
               29  CGCG                
NsiI          619  ATGCAT               ATGCA^T
              971  ATGCAT              
PaeR7I       1301  CTCGAG               C^TCGAG
             1676  CTCGAG              
PinAI          80  ACCGGT               A^CCGGT
             1532  ACCGGT              
PleI          180  GAGTC                GAGTC(4/5)
             1304  GAGTC               
PstI           37  CTGCAG               CTGCA^G
             1460  CTGCAG              
Sau96I        472  GGTCC                G^G[ACGT]CC
              725  GGACC               
SfcI           37  CTGCAG               C^T[GA][CT]AG
             1460  CTGCAG              
XcmI         1311  CCATGCTGGATTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1685  CCAAGATTCTTTTGG     
XhoI         1301  CTCGAG               C^TCGAG
             1676  CTCGAG              
XhoII         801  GGATCT               [GA]^GATC[CT]
             1389  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        ApoI        
AspHI       BbsI        BbvI        BfaI        BmyI        
BpuAI       BsaJI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspMI       BsrFI       
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr10I      DdeI        DpnII       EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MnlI        MseI        
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        Sau3AI      ScrFI       SfaNI       
SmlI        SspI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp509I     TspRI       TthHB8I     
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