Restriction map for pF1KB6189
 (Linear) (Six-base) MAPSORT of: KIBB6189.seq  Check: 7307  from: 1  to: 1980



KIBB6189.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     761    1056    1980 
   Size:        761     295     924

  Fragments arranged by size: 

                 924     761     295
 
AatII G_ACGT'C

Cuts at:      0     983    1980 
   Size:        983     997
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     790    1980 
   Size:        169     621    1190

  Fragments arranged by size: 

                1190     621     169
 
AflII C'TTAA_G

Cuts at:      0     495    1980 
   Size:        495    1485
 
AflIII A'CryG_T

Cuts at:      0    1360    1892    1980 
   Size:       1360     532      88

  Fragments arranged by size: 

                1360     532      88
 
AgeI A'CCGG_T

Cuts at:      0      80    1532    1980 
   Size:         80    1452     448

  Fragments arranged by size: 

                1452     448      80
 
AhdI GACnn_n'nnGTC

Cuts at:      0     311    1980 
   Size:        311    1669
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1924    1980 
   Size:       1924      56
 
ApaLI G'TGCA_C

Cuts at:      0    1240    1980 
   Size:       1240     740
 
ApoI r'AATT_y

Cuts at:      0     117     141     676     763     853    1042    1980 
   Size:        117      24     535      87      90     189     938

  Fragments arranged by size: 

                 938     535     189     117      90      87      24
 
AseI AT'TA_AT

Cuts at:      0     485    1980 
   Size:        485    1495
 
AvaI C'yCGr_G

Cuts at:      0    1301    1676    1980 
   Size:       1301     375     304

  Fragments arranged by size: 

                1301     375     304
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     175     523    1299    1346    1980 
   Size:        175     348     776      47     634

  Fragments arranged by size: 

                 776     634     348     175      47
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     726     760    1980 
   Size:        726      34    1220

  Fragments arranged by size: 

                1220     726      34
 
BfrBI ATG'CAT

Cuts at:      0     621     973    1980 
   Size:        621     352    1007

  Fragments arranged by size: 

                1007     621     352
 
Bme1580I G_kGCm'C

Cuts at:      0    1244    1980 
   Size:       1244     736
 
BmgBI CAC'GTC

Cuts at:      0     693    1636    1980 
   Size:        693     943     344

  Fragments arranged by size: 

                 943     693     344
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     723     729    1980 
   Size:        723       6    1251

  Fragments arranged by size: 

                1251     723       6
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     874    1980 
   Size:        874    1106
 
Bpu10I CC'TnA_GC

Cuts at:      0     697    1980 
   Size:        697    1283
 
BsaHI Gr'CG_yC

Cuts at:      0     980    1980 
   Size:        980    1000
 
BsaWI w'CCGG_w

Cuts at:      0      80    1532    1980 
   Size:         80    1452     448

  Fragments arranged by size: 

                1452     448      80
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     580     610    1537    1567    1980 
   Size:        580      30     927      30     413

  Fragments arranged by size: 

                 927     580     413      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     284     862    1980 
   Size:        284     578    1118

  Fragments arranged by size: 

                1118     578     284
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1084    1980 
   Size:       1084     896
 
BsiHKAI G_wGCw'C

Cuts at:      0      25     698    1244    1980 
   Size:         25     673     546     736

  Fragments arranged by size: 

                 736     673     546      25
 
BsmI GAATG_Cn'

Cuts at:      0    1479    1980 
   Size:       1479     501
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     987    1980 
   Size:        987     993
 
Bsp1286I G_dGCh'C

Cuts at:      0      25     698    1244    1980 
   Size:         25     673     546     736

  Fragments arranged by size: 

                 736     673     546      25
 
BspHI T'CATG_A

Cuts at:      0    1701    1980 
   Size:       1701     279
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     564     761    1056    1980 
   Size:        564     197     295     924

  Fragments arranged by size: 

                 924     564     295     197
 
BsrFI r'CCGG_y

Cuts at:      0      80    1532    1724    1980 
   Size:         80    1452     192     256

  Fragments arranged by size: 

                1452     256     192      80
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1924    1980 
   Size:       1924      56
 
BstBI TT'CG_AA

Cuts at:      0     174    1980 
   Size:        174    1806
 
BstYI r'GATC_y

Cuts at:      0     801    1389    1980 
   Size:        801     588     591

  Fragments arranged by size: 

                 801     591     588
 
Bsu36I CC'TnA_GG

Cuts at:      0     634    1980 
   Size:        634    1346
 
BtgI C'CryG_G

Cuts at:      0    1418    1980 
   Size:       1418     562
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     729    1980 
   Size:        729    1251
 
DraI TTT'AAA

Cuts at:      0     146    1356    1980 
   Size:        146    1210     624

  Fragments arranged by size: 

                1210     624     146
 
EaeI y'GGCC_r

Cuts at:      0    1640    1934    1980 
   Size:       1640     294      46

  Fragments arranged by size: 

                1640     294      46
 
EarI CTCTTCn'nnn_

Cuts at:      0      44     826    1980 
   Size:         44     782    1154

  Fragments arranged by size: 

                1154     782      44
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     790     874    1980 
   Size:        169     621      84    1106

  Fragments arranged by size: 

                1106     621     169      84
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     241    1980 
   Size:        241    1739
 
EcoO109I rG'GnC_Cy

Cuts at:      0     725    1980 
   Size:        725    1255
 
EcoRV GAT'ATC

Cuts at:      0    1950    1980 
   Size:       1950      30
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      43      45      75      77     297     329     496     528 
   Size:         43       2      30       2     220      32     167      32

Cuts at:    528     580     612    1436    1468    1980 
   Size:         52      32     824      32     512

  Fragments arranged by size: 

                 824     512     220     167      52      43      32      32
                  32      32      30       2       2
 
HincII GTy'rAC

Cuts at:      0     588     641    1980 
   Size:        588      53    1339

  Fragments arranged by size: 

                1339     588      53
 
HindIII A'AGCT_T

Cuts at:      0     917     925    1980 
   Size:        917       8    1055

  Fragments arranged by size: 

                1055     917       8
 
MfeI C'AATT_G

Cuts at:      0     520    1980 
   Size:        520    1460
 
MscI TGG'CCA

Cuts at:      0    1642    1936    1980 
   Size:       1642     294      44

  Fragments arranged by size: 

                1642     294      44
 
MslI CAynn'nnrTG

Cuts at:      0     873    1980 
   Size:        873    1107
 
MspA1I CmG'CkG

Cuts at:      0    1591    1980 
   Size:       1591     389
 
NcoI C'CATG_G

Cuts at:      0    1418    1980 
   Size:       1418     562
 
NsiI A_TGCA'T

Cuts at:      0     623     975    1980 
   Size:        623     352    1005

  Fragments arranged by size: 

                1005     623     352
 
NspI r_CATG'y

Cuts at:      0     625     741    1896    1980 
   Size:        625     116    1155      84

  Fragments arranged by size: 

                1155     625     116      84
 
PasI CC'CwG_GG

Cuts at:      0    1099    1980 
   Size:       1099     881
 
PciI A'CATG_T

Cuts at:      0    1892    1980 
   Size:       1892      88
 
PpuMI rG'GwC_Cy

Cuts at:      0     725    1980 
   Size:        725    1255
 
PsiI TTA'TAA

Cuts at:      0     818    1980 
   Size:        818    1162
 
PspXI vC'TCGA_Gb

Cuts at:      0    1301    1980 
   Size:       1301     679
 
PstI C_TGCA'G

Cuts at:      0      41    1464    1980 
   Size:         41    1423     516

  Fragments arranged by size: 

                1423     516      41
 
PvuII CAG'CTG

Cuts at:      0    1591    1980 
   Size:       1591     389
 
SanDI GG'GwC_CC

Cuts at:      0     725    1980 
   Size:        725    1255
 
SfcI C'TryA_G

Cuts at:      0      37    1460    1980 
   Size:         37    1423     520

  Fragments arranged by size: 

                1423     520      37
 
SmlI C'TyrA_G

Cuts at:      0     495    1301    1676    1980 
   Size:        495     806     375     304

  Fragments arranged by size: 

                 806     495     375     304
 
SphI G_CATG'C

Cuts at:      0     741    1980 
   Size:        741    1239
 
SspI AAT'ATT

Cuts at:      0     168    1256    1647    1880    1980 
   Size:        168    1088     391     233     100

  Fragments arranged by size: 

                1088     391     233     168     100
 
StyI C'CwwG_G

Cuts at:      0     380    1418    1937    1980 
   Size:        380    1038     519      43

  Fragments arranged by size: 

                1038     519     380      43
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     325     357    1223    1255    1310    1342    1980 
   Size:        325      32     866      32      55      32     638

  Fragments arranged by size: 

                 866     638     325      55      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1450    1980 
   Size:       1450     530
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1318    1692    1980 
   Size:       1318     374     288

  Fragments arranged by size: 

                1318     374     288
 
XhoI C'TCGA_G

Cuts at:      0    1301    1676    1980 
   Size:       1301     375     304

  Fragments arranged by size: 

                1301     375     304
 
ZraI GAC'GTC

Cuts at:      0     981    1980 
   Size:        981     999
 

 Enzymes that do cut:

     AarI    AatII     AcuI    AflII   AflIII     AgeI     AhdI    AlwNI
    ApaLI     ApoI     AseI     AvaI     BbsI     BcgI    BfrBI Bme1580I
    BmgBI     BmrI     BpmI   Bpu10I    BsaHI    BsaWI    BsaXI    BseRI
     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrFI
   BstAPI    BstBI    BstYI   Bsu36I     BtgI    BtgZI     DraI     EaeI
     EarI  Eco57MI    EcoNI EcoO109I    EcoRV    Hin4I   HincII  HindIII
     MfeI     MscI     MslI   MspA1I     NcoI     NsiI     NspI     PasI
     PciI    PpuMI     PsiI    PspXI     PstI    PvuII    SanDI     SfcI
     SmlI     SphI     SspI     StyI     TstI  Tth111I     XcmI     XhoI
     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AfeI     AleI     AlfI     AloI     ApaI
     AscI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI    BciVI     BclI     BglI    BglII     BlpI     BmtI     BplI
    BpuEI     BsaI    BsaAI    BsaBI    BseYI    BsiEI    BsiWI    BspEI
    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstEII    BstXI  BstZ17I
     BtsI     ClaI    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoRI     FalI     FseI     FspI    FspAI    HaeII     HpaI     KasI
     KpnI     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     PacI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI   PspOMI     PsrI     PvuI    RsrII     SacI    SacII     SalI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     StuI     SwaI    TaqII     TatI
     XbaI     XmaI     XmnI
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