Restriction map for pF1KB6188
 (Linear) (Six-base) MAPSORT of: KIBB6188.seq  Check: 7598  from: 1  to: 2109



KIBB6188.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1811    2109 
   Size:       1811     298
 
AclI AA'CG_TT

Cuts at:      0     449    2109 
   Size:        449    1660
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     238     599     896     977     997    1244    2109 
   Size:        238     361     297      81      20     247     865

  Fragments arranged by size: 

                 865     361     297     247     238      81      20
 
AflIII A'CryG_T

Cuts at:      0      40    2109 
   Size:         40    2069
 
AleI CACnn'nnGTG

Cuts at:      0    1957    2109 
   Size:       1957     152
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     724    2109 
   Size:        724    1385
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1056    2053    2109 
   Size:       1056     997      56

  Fragments arranged by size: 

                1056     997      56
 
ApaI G_GGCC'C

Cuts at:      0     679    1381    1602    1905    2109 
   Size:        679     702     221     303     204

  Fragments arranged by size: 

                 702     679     303     221     204
 
AvaI C'yCGr_G

Cuts at:      0    1905    2109 
   Size:       1905     204
 
BanI G'GyrC_C

Cuts at:      0     387    1447    2109 
   Size:        387    1060     662

  Fragments arranged by size: 

                1060     662     387
 
BanII G_rGCy'C

Cuts at:      0     221     679    1163    1381    1391    1602    1905    2109 
   Size:        221     458     484     218      10     211     303     204

  Fragments arranged by size: 

                 484     458     303     221     218     211     204      10
            
 
BbeI G_GCGC'C

Cuts at:      0    1451    2109 
   Size:       1451     658
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     379     601    1197    1772    2109 
   Size:        379     222     596     575     337

  Fragments arranged by size: 

                 596     575     379     337     222
 
BbvCI CC'TCA_GC

Cuts at:      0     298    2109 
   Size:        298    1811
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1693    1727    2109 
   Size:       1693      34     382

  Fragments arranged by size: 

                1693     382      34
 
BclI T'GATC_A

Cuts at:      0     995    2109 
   Size:        995    1114
 
BglI GCCn_nnn'nGGC

Cuts at:      0     879    1144    2065    2074    2109 
   Size:        879     265     921       9      35

  Fragments arranged by size: 

                 921     879     265      35       9
 
BglII A'GATC_T

Cuts at:      0     249    2109 
   Size:        249    1860
 
BlpI GC'TnA_GC

Cuts at:      0      19      64    2109 
   Size:         19      45    2045

  Fragments arranged by size: 

                2045      45      19
 
Bme1580I G_kGCm'C

Cuts at:      0     679    1381    1602    1905    1988    2109 
   Size:        679     702     221     303      83     121

  Fragments arranged by size: 

                 702     679     303     221     121      83
 
BmgBI CAC'GTC

Cuts at:      0     192    2109 
   Size:        192    1917
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      87     823     832    1409    2109 
   Size:         87     736       9     577     700

  Fragments arranged by size: 

                 736     700     577      87       9
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     540    1078    1153    1345    1625    2109 
   Size:        540     538      75     192     280     484

  Fragments arranged by size: 

                 540     538     484     280     192      75
 
Bpu10I CC'TnA_GC

Cuts at:      0     298    2109 
   Size:        298    1811
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1147    2109 
   Size:       1147     962
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1463    1876    2109 
   Size:       1463     413     233

  Fragments arranged by size: 

                1463     413     233
 
BsaBI GATnn'nnATC

Cuts at:      0    1930    1966    2109 
   Size:       1930      36     143

  Fragments arranged by size: 

                1930     143      36
 
BsaHI Gr'CG_yC

Cuts at:      0    1448    2109 
   Size:       1448     661
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     166     196     386     416     566     596    1819    1849 
   Size:        166      30     190      30     150      30    1223      30

Cuts at:   1849    2062    2092    2109 
   Size:        213      30      17

  Fragments arranged by size: 

                1223     213     190     166     150      30      30      30
                  30      30      17
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     142     282     543     592     684    1265    1482    1933 
   Size:        142     140     261      49      92     581     217     451

Cuts at:   1933    2109 
   Size:        176

  Fragments arranged by size: 

                 581     451     261     217     176     142     140      92
                  49
 
BseYI C'CCAG_C

Cuts at:      0     639     787    1317    1391    1396    1553    1812    2109 
   Size:        639     148     530      74       5     157     259     297

  Fragments arranged by size: 

                 639     530     297     259     157     148      74       5
            
 
BsiHKAI G_wGCw'C

Cuts at:      0     365    1163    2109 
   Size:        365     798     946

  Fragments arranged by size: 

                 946     798     365
 
BsmI GAATG_Cn'

Cuts at:      0    1116    1738    2109 
   Size:       1116     622     371

  Fragments arranged by size: 

                1116     622     371
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     322    2109 
   Size:        322    1787
 
Bsp1286I G_dGCh'C

Cuts at:      0     221     365     679    1163    1381    1391    1602    1905 
   Size:        221     144     314     484     218      10     211     303

Cuts at:   1905    1988    2109 
   Size:         83     121

  Fragments arranged by size: 

                 484     314     303     221     218     211     144     121
                  83      10
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      68    1811    2109 
   Size:         68    1743     298

  Fragments arranged by size: 

                1743     298      68
 
BsrBI CCG'CTC

Cuts at:      0     928    2109 
   Size:        928    1181
 
BsrDI GCAATG_nn'

Cuts at:      0    1217    2031    2109 
   Size:       1217     814      78

  Fragments arranged by size: 

                1217     814      78
 
BsrFI r'CCGG_y

Cuts at:      0     882    1502    2109 
   Size:        882     620     607

  Fragments arranged by size: 

                 882     620     607
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1669    2109 
   Size:       1669     440
 
BstYI r'GATC_y

Cuts at:      0     249    1586    2109 
   Size:        249    1337     523

  Fragments arranged by size: 

                1337     523     249
 
Bsu36I CC'TnA_GG

Cuts at:      0     696    1382    1528    2109 
   Size:        696     686     146     581

  Fragments arranged by size: 

                 696     686     581     146
 
BtgI C'CryG_G

Cuts at:      0     223     898    1624    1868    1896    2109 
   Size:        223     675     726     244      28     213

  Fragments arranged by size: 

                 726     675     244     223     213      28
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     296    2109 
   Size:        296    1813
 
BtsI GCAGTG_nn'

Cuts at:      0     727    2109 
   Size:        727    1382
 
EaeI y'GGCC_r

Cuts at:      0     122    2109 
   Size:        122    1987
 
EarI CTCTTCn'nnn_

Cuts at:      0     148     291     907    1219    1489    2109 
   Size:        148     143     616     312     270     620

  Fragments arranged by size: 

                 620     616     312     270     148     143
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19    1090    2109 
   Size:         19    1071    1019

  Fragments arranged by size: 

                1071    1019      19
 
EcoICRI GAG'CTC

Cuts at:      0    1161    2109 
   Size:       1161     948
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     238     540     599     896     977     997    1078    1153 
   Size:        238     302      59     297      81      20      81      75

Cuts at:   1153    1244    1345    1625    2109 
   Size:         91     101     280     484

  Fragments arranged by size: 

                 484     302     297     280     238     101      91      81
                  81      75      59      20
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     850    2109 
   Size:        850    1259
 
EcoO109I rG'GnC_Cy

Cuts at:      0     582    1137    1367    1377    1378    1598    1901    1902 
   Size:        582     555     230      10       1     220     303       1

Cuts at:   1902    2109 
   Size:        207

  Fragments arranged by size: 

                 582     555     303     230     220     207      10       1
                   1
 
HaeII r_GCGC'y

Cuts at:      0    1451    1566    2109 
   Size:       1451     115     543

  Fragments arranged by size: 

                1451     543     115
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     166     198     406     438     457     489     566     592 
   Size:        166      32     208      32      19      32      77      26

Cuts at:    592     598     624     679     711    1873    1905    2109 
   Size:          6      26      55      32    1162      32     204

  Fragments arranged by size: 

                1162     208     204     166      77      55      32      32
                  32      32      32      26      26      19       6
 
HincII GTy'rAC

Cuts at:      0     522    2109 
   Size:        522    1587
 
KasI G'GCGC_C

Cuts at:      0    1447    2109 
   Size:       1447     662
 
MfeI C'AATT_G

Cuts at:      0    1751    2109 
   Size:       1751     358
 
MluI A'CGCG_T

Cuts at:      0      40    2109 
   Size:         40    2069
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1686    2109 
   Size:       1686     423
 
MslI CAynn'nnrTG

Cuts at:      0    1957    2109 
   Size:       1957     152
 
MspA1I CmG'CkG

Cuts at:      0    1701    2109 
   Size:       1701     408
 
NaeI GCC'GGC

Cuts at:      0     884    1504    2109 
   Size:        884     620     605

  Fragments arranged by size: 

                 884     620     605
 
NarI GG'CG_CC

Cuts at:      0    1448    2109 
   Size:       1448     661
 
NcoI C'CATG_G

Cuts at:      0     223    1868    2109 
   Size:        223    1645     241

  Fragments arranged by size: 

                1645     241     223
 
NgoMIV G'CCGG_C

Cuts at:      0     882    1502    2109 
   Size:        882     620     607

  Fragments arranged by size: 

                 882     620     607
 
PasI CC'CwG_GG

Cuts at:      0    1374    1441    2109 
   Size:       1374      67     668

  Fragments arranged by size: 

                1374     668      67
 
PfoI T'CCnGG_A

Cuts at:      0     584    1024    2109 
   Size:        584     440    1085

  Fragments arranged by size: 

                1085     584     440
 
PpuMI rG'GwC_Cy

Cuts at:      0     582    1367    2109 
   Size:        582     785     742

  Fragments arranged by size: 

                 785     742     582
 
PshAI GACnn'nnGTC

Cuts at:      0      80    1614    2109 
   Size:         80    1534     495

  Fragments arranged by size: 

                1534     495      80
 
PspOMI G'GGCC_C

Cuts at:      0     675    1377    1598    1901    2109 
   Size:        675     702     221     303     208

  Fragments arranged by size: 

                 702     675     303     221     208
 
PstI C_TGCA'G

Cuts at:      0      89     734     809    1052    2058    2109 
   Size:         89     645      75     243    1006      51

  Fragments arranged by size: 

                1006     645     243      89      75      51
 
PvuII CAG'CTG

Cuts at:      0    1701    2109 
   Size:       1701     408
 
SacI G_AGCT'C

Cuts at:      0    1163    2109 
   Size:       1163     946
 
SapI GCTCTTCn'nnn_

Cuts at:      0     148     291     907    1489    2109 
   Size:        148     143     616     582     620

  Fragments arranged by size: 

                 620     616     582     148     143
 
ScaI AGT'ACT

Cuts at:      0    1079    2109 
   Size:       1079    1030
 
SfcI C'TryA_G

Cuts at:      0      85     730     743     805    1048    2054    2109 
   Size:         85     645      13      62     243    1006      55

  Fragments arranged by size: 

                1006     645     243      85      62      55      13
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    2074    2109 
   Size:       2074      35
 
SfoI GGC'GCC

Cuts at:      0    1449    2109 
   Size:       1449     660
 
SmaI CCC'GGG

Cuts at:      0    1907    2109 
   Size:       1907     202
 
SmlI C'TyrA_G

Cuts at:      0    1162    2109 
   Size:       1162     947
 
StuI AGG'CCT

Cuts at:      0     532    2077    2109 
   Size:        532    1545      32

  Fragments arranged by size: 

                1545     532      32
 
StyI C'CwwG_G

Cuts at:      0     223     383    1451    1868    2109 
   Size:        223     160    1068     417     241

  Fragments arranged by size: 

                1068     417     241     223     160
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1733    1772    2109 
   Size:       1733      39     337

  Fragments arranged by size: 

                1733     337      39
 
TatI w'GTAC_w

Cuts at:      0    1077    2109 
   Size:       1077    1032
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     386     418    2062    2094    2109 
   Size:        386      32    1644      32      15

  Fragments arranged by size: 

                1644     386      32      32      15
 
Tth111I GACn'n_nGTC

Cuts at:      0     378     600    1887    2109 
   Size:        378     222    1287     222

  Fragments arranged by size: 

                1287     378     222     222
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1851    2109 
   Size:       1851     258
 
XmaI C'CCGG_G

Cuts at:      0    1905    2109 
   Size:       1905     204
 

 Enzymes that do cut:

     AarI     AclI     AcuI   AflIII     AleI     AlfI    AlwNI     ApaI
     AvaI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I
    BpuEI     BsaI    BsaBI    BsaHI    BsaXI    BseRI    BseYI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     EaeI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I   HincII     KasI
     MfeI     MluI     MmeI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     PasI     PfoI    PpuMI    PshAI   PspOMI     PstI    PvuII
     SacI     SapI     ScaI     SfcI     SfiI     SfoI     SmaI     SmlI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AfeI    AflII     AgeI     AhdI     AloI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
    BciVI    BfrBI     BmtI     BplI    BsaAI    BsaWI     BsgI    BsiEI
    BsiWI    BspEI    BspHI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I     ClaI    CspCI     DraI   DraIII     DrdI     EagI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI  HindIII     HpaI
     KpnI     MscI     NdeI     NheI     NotI     NruI     NsiI     NspI
     PacI     PciI    PflMI     PmeI     PmlI     PpiI     PsiI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SbfI    SexAI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     XbaI     XhoI     XmnI     ZraI
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