Restriction map for pF1KB6174
##################################
# NO CUT ENZYMES (  118 enzymes) #
##################################

AatII       Acc65I      AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AviII       AvrII       BbrPI       
BcgI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsiEI       BsiWI       
BsmI        Bsp1407I    BspDI       BspHI       BspLU11I    
BsrBI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FseI        FspI        HincII      HindII      
HindIII     HpaI        KpnI        KspI        MamI        
MfeI        MluI        MunI        NcoI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
PvuI        RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        Tth111I     XbaI        
XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccII          52  CGCG                 CG^CG
AccIII        647  TCCGGA               T^CCGGA
AclI          453  AACGTT               AA^CGTT
AcsI         1156  AAATTC               [GA]^AATT[CT]
ApoI         1156  AAATTC               [GA]^AATT[CT]
Asp700I        55  GAAGTCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          333  CCCGAG               C^[CT]CG[GA]G
BalI          711  TGGCCA               TGG^CCA
BamHI         125  GGATCC               G^GATCC
BbsI          556  GAAGAC               GAAGAC(2/6)
BbvCI           4  GCTGAGG              CCTCAGC(-5/-2)
BciVI        1584  GGATAC               GTATCC(6/5)
BclI         1790  TGATCA               T^GATCA
BpmI         1874  CTGGAG               CTGGAG(16/14)
BpuAI         556  GAAGAC               GAAGAC(2/6)
BsaWI         647  TCCGGA               [AT]^CCGG[AT]
BseAI         647  TCCGGA               T^CCGGA
BsmBI         636  GAGACG               CGTCTC(1/5)
BsmFI         963  GTCCC                GGGAC(10/14)
BsoBI         333  CCCGAG               C^[CT]CG[GA]G
BspEI         647  TCCGGA               T^CCGGA
BsrDI         715  CATTGC               GCAATG(2/0)
BsrFI        1070  GCCGGC               [GA]^CCGG[CT]
BstAPI        200  GCATCTTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI          52  CGCG                 CG^CG
BstYI         125  GGATCC               [GA]^GATC[CT]
Cfr10I       1070  GCCGGC               [GA]^CCGG[CT]
DsaI          233  CCGTGG               C^C[GA][CT]GG
EcoNI        1512  CCTGTTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI         1790  TGATCA               T^GATCA
MflI          125  GGATCC               [GA]^GATC[CT]
MluNI         711  TGGCCA               TGG^CCA
MroI          647  TCCGGA               T^CCGGA
MscI          711  TGGCCA               TGG^CCA
MslI         1782  CACACCCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI           52  CGCG                 CG^CG
NaeI         1070  GCCGGC               GCC^GGC
NgoMIV       1070  GCCGGC               G^CCGGC
NspI          873  ACATGC               [GA]CATG^[CT]
Psp1406I      453  AACGTT               AA^CGTT
StuI         1671  AGGCCT               AGG^CCT
Tsp45I        896  GTGAC                ^GT[GC]AC
XcmI          699  CCAAGAGGATTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         125  GGATCC               [GA]^GATC[CT]
XmnI           55  GAAGTCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccI         1036  GTAGAC               GT^[AC][GT]AC
             1663  GTAGAC              
AspHI         473  GAGCAC               G[AT]GC[AT]^C
             1530  GTGCTC              
AvaII         301  GGACC                G^G[AT]CC
             1853  GGTCC               
BfaI         1652  CTAG                 C^TAG
             1880  CTAG                
BglI          217  GCCCCCAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              264  GCCTTCAAGGC         
BsaI          343  GAGACC               GGTCTC(1/5)
              748  GGTCTC              
BseRI        1708  GAGGAG               GAGGAG(10/8)
             1720  GAGGAG              
BsiHKAI       473  GAGCAC               G[AT]GC[AT]^C
             1530  GTGCTC              
BstXI         989  CCAGAGCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1527  CCAGTGCTCTGG        
EaeI          711  TGGCCA               [CT]^GGCC[GA]
             1125  TGGCCG              
EarI          181  CTCTTC               CTCTTC(1/4)
             1762  GAAGAG              
Ksp632I       181  CTCTTC               CTCTTC(1/4)
             1762  GAAGAG              
MaeI         1652  CTAG                 C^TAG
             1880  CTAG                
MaeIII        550  GTAAC                ^GT[ACGT]AC
              896  GTGAC               
MseI          512  TTAA                 T^TAA
             1865  TTAA                
PstI          586  CTGCAG               CTGCA^G
             1207  CTGCAG              
PvuII         589  CAGCTG               CAG^CTG
             1613  CAGCTG              
SfaNI         200  GCATC                GCATC(5/9)
              607  GCATC               
TfiI          410  GAATC                G^A[AT]TC
             1735  GATTC               
Tru9I         512  TTAA                 T^TAA
             1865  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   75 enzymes) #
#########################################

AciI        AcyI        AluI        AlwI        AlwNI       
ApaI        BanI        BanII       BbeI        BbvI        
BcnI        BmyI        BsaHI       BsaJI       BsgI        
BsiYI       BslI        BsmAI       Bsp1286I    BspMI       
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        KasI        MaeII       MboI        
MboII       MnlI        MspA1I      MspI        MvaI        
MwoI        NarI        NciI        NdeII       NlaIII      
NlaIV       PflMI       PleI        Sau3AI      Sau96I      
ScrFI       SfcI        SfoI        StyI        TaqI        
TseI        Tsp509I     TspRI       TthHB8I     Van91I      
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