Restriction map for pF1KB6171
 (Linear) (Six-base) MAPSORT of: KIBB6171.seq  Check: 2414  from: 1  to: 1947



KIBB6171.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1838    1947 
   Size:       1838     109
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     394     639    1947 
   Size:        394     245    1308

  Fragments arranged by size: 

                1308     394     245
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1525    1557    1947 
   Size:       1525      32     390

  Fragments arranged by size: 

                1525     390      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1935    1947 
   Size:       1935      12
 
ApaI G_GGCC'C

Cuts at:      0      58    1947 
   Size:         58    1889
 
ApaLI G'TGCA_C

Cuts at:      0    1113    1947 
   Size:       1113     834
 
ApoI r'AATT_y

Cuts at:      0     810    1947 
   Size:        810    1137
 
AvaI C'yCGr_G

Cuts at:      0     660    1947 
   Size:        660    1287
 
AvrII C'CTAG_G

Cuts at:      0    1842    1947 
   Size:       1842     105
 
BanI G'GyrC_C

Cuts at:      0       3      63     720    1947 
   Size:          3      60     657    1227

  Fragments arranged by size: 

                1227     657      60       3
 
BanII G_rGCy'C

Cuts at:      0      58    1103    1558    1946    1947 
   Size:         58    1045     455     388       1

  Fragments arranged by size: 

                1045     455     388      58       1
 
BbeI G_GCGC'C

Cuts at:      0      67    1947 
   Size:         67    1880
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     216    1647    1947 
   Size:        216    1431     300

  Fragments arranged by size: 

                1431     300     216
 
BbvCI CC'TCA_GC

Cuts at:      0     758    1947 
   Size:        758    1189
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1019    1947 
   Size:       1019     928
 
BclI T'GATC_A

Cuts at:      0     489     986    1947 
   Size:        489     497     961

  Fragments arranged by size: 

                 961     497     489
 
BfrBI ATG'CAT

Cuts at:      0     837    1947 
   Size:        837    1110
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1604    1766    1947 
   Size:       1604     162     181

  Fragments arranged by size: 

                1604     181     162
 
BglII A'GATC_T

Cuts at:      0    1175    1947 
   Size:       1175     772
 
Bme1580I G_kGCm'C

Cuts at:      0      58    1117    1230    1859    1947 
   Size:         58    1059     113     629      88

  Fragments arranged by size: 

                1059     629     113      88      58
 
BmgBI CAC'GTC

Cuts at:      0    1134    1947 
   Size:       1134     813
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1224    1947 
   Size:       1224     723
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     748     995    1156    1820    1947 
   Size:        748     247     161     664     127

  Fragments arranged by size: 

                 748     664     247     161     127
 
Bpu10I CC'TnA_GC

Cuts at:      0     758     980    1947 
   Size:        758     222     967

  Fragments arranged by size: 

                 967     758     222
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     352     868    1947 
   Size:        352     516    1079

  Fragments arranged by size: 

                1079     516     352
 
BsaBI GATnn'nnATC

Cuts at:      0    1007    1947 
   Size:       1007     940
 
BsaHI Gr'CG_yC

Cuts at:      0      64    1947 
   Size:         64    1883
 
BsaWI w'CCGG_w

Cuts at:      0     266     467    1947 
   Size:        266     201    1480

  Fragments arranged by size: 

                1480     266     201
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     968     998    1420    1450    1947 
   Size:        968      30     422      30     497

  Fragments arranged by size: 

                 968     497     422      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     151     208     775    1947 
   Size:        151      57     567    1172

  Fragments arranged by size: 

                1172     567     151      57
 
BseYI C'CCAG_C

Cuts at:      0    1338    1557    1602    1947 
   Size:       1338     219      45     345

  Fragments arranged by size: 

                1338     345     219      45
 
BsiEI CG_ry'CG

Cuts at:      0     147    1947 
   Size:        147    1800
 
BsiHKAI G_wGCw'C

Cuts at:      0     622    1117    1946    1947 
   Size:        622     495     829       1

  Fragments arranged by size: 

                 829     622     495       1
 
BsmI GAATG_Cn'

Cuts at:      0    1073    1947 
   Size:       1073     874
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     441    1947 
   Size:        441    1506
 
Bsp1286I G_dGCh'C

Cuts at:      0      58     622    1103    1117    1230    1558    1859    1946 
   Size:         58     564     481      14     113     328     301      87

Cuts at:   1946    1947 
   Size:          1

  Fragments arranged by size: 

                 564     481     328     301     113      87      58      14
                   1
 
BspEI T'CCGG_A

Cuts at:      0     266    1947 
   Size:        266    1681
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     784    1838    1947 
   Size:        784    1054     109

  Fragments arranged by size: 

                1054     784     109
 
BsrDI GCAATG_nn'

Cuts at:      0    1055    1679    1947 
   Size:       1055     624     268

  Fragments arranged by size: 

                1055     624     268
 
BsrFI r'CCGG_y

Cuts at:      0    1466    1947 
   Size:       1466     481
 
BsrGI T'GTAC_A

Cuts at:      0     929    1947 
   Size:        929    1018
 
BssSI C'ACGA_G

Cuts at:      0     961    1947 
   Size:        961     986
 
BstEII G'GTnAC_C

Cuts at:      0     771    1053    1947 
   Size:        771     282     894

  Fragments arranged by size: 

                 894     771     282
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     600    1401    1947 
   Size:        600     801     546

  Fragments arranged by size: 

                 801     600     546
 
BstYI r'GATC_y

Cuts at:      0    1175    1540    1947 
   Size:       1175     365     407

  Fragments arranged by size: 

                1175     407     365
 
Bsu36I CC'TnA_GG

Cuts at:      0      59    1947 
   Size:         59    1888
 
BtgI C'CryG_G

Cuts at:      0     114     141     188     893    1947 
   Size:        114      27      47     705    1054

  Fragments arranged by size: 

                1054     705     114      47      27
 
BtsI GCAGTG_nn'

Cuts at:      0     214    1947 
   Size:        214    1733
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1190    1225    1575    1610    1947 
   Size:       1190      35     350      35     337

  Fragments arranged by size: 

                1190     350     337      35      35
 
DraI TTT'AAA

Cuts at:      0    1377    1947 
   Size:       1377     570
 
EaeI y'GGCC_r

Cuts at:      0     144    1411    1502    1596    1947 
   Size:        144    1267      91      94     351

  Fragments arranged by size: 

                1267     351     144      94      91
 
EagI C'GGCC_G

Cuts at:      0     144    1947 
   Size:        144    1803
 
EarI CTCTTCn'nnn_

Cuts at:      0     935    1328    1947 
   Size:        935     393     619

  Fragments arranged by size: 

                 935     619     393
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     433    1947 
   Size:        433    1514
 
EcoICRI GAG'CTC

Cuts at:      0    1944    1947 
   Size:       1944       3
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     394     639     748     995    1156    1820    1947 
   Size:        394     245     109     247     161     664     127

  Fragments arranged by size: 

                 664     394     247     245     161     127     109
 
EcoO109I rG'GnC_Cy

Cuts at:      0      55     369    1901    1947 
   Size:         55     314    1532      46

  Fragments arranged by size: 

                1532     314      55      46
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1515    1947 
   Size:       1515     432
 
HaeII r_GCGC'y

Cuts at:      0      67     411    1947 
   Size:         67     344    1536

  Fragments arranged by size: 

                1536     344      67
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       2      34      42      74     406     438     474     506 
   Size:          2      32       8      32     332      32      36      32

Cuts at:    506     778     810     978    1010    1310    1342    1657    1689 
   Size:        272      32     168      32     300      32     315      32

Cuts at:   1689    1947 
   Size:        258

  Fragments arranged by size: 

                 332     315     300     272     258     168      36      32
                  32      32      32      32      32      32      32       8
                   2
 
KasI G'GCGC_C

Cuts at:      0      63    1947 
   Size:         63    1884
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     636     659    1610    1934    1947 
   Size:        636      23     951     324      13

  Fragments arranged by size: 

                 951     636     324      23      13
 
MscI TGG'CCA

Cuts at:      0    1413    1504    1598    1947 
   Size:       1413      91      94     349

  Fragments arranged by size: 

                1413     349      94      91
 
MslI CAynn'nnrTG

Cuts at:      0     133     892    1947 
   Size:        133     759    1055

  Fragments arranged by size: 

                1055     759     133
 
MspA1I CmG'CkG

Cuts at:      0     143     477    1602    1947 
   Size:        143     334    1125     345

  Fragments arranged by size: 

                1125     345     334     143
 
NaeI GCC'GGC

Cuts at:      0    1468    1947 
   Size:       1468     479
 
NarI GG'CG_CC

Cuts at:      0      64    1947 
   Size:         64    1883
 
NcoI C'CATG_G

Cuts at:      0     114     893    1947 
   Size:        114     779    1054

  Fragments arranged by size: 

                1054     779     114
 
NgoMIV G'CCGG_C

Cuts at:      0    1466    1947 
   Size:       1466     481
 
NsiI A_TGCA'T

Cuts at:      0     839    1947 
   Size:        839    1108
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1712    1935    1947 
   Size:       1712     223      12

  Fragments arranged by size: 

                1712     223      12
 
PfoI T'CCnGG_A

Cuts at:      0     400     424    1328    1947 
   Size:        400      24     904     619

  Fragments arranged by size: 

                 904     619     400      24
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     421     453    1947 
   Size:        421      32    1494

  Fragments arranged by size: 

                1494     421      32
 
PspOMI G'GGCC_C

Cuts at:      0      54    1947 
   Size:         54    1893
 
PstI C_TGCA'G

Cuts at:      0     288    1257    1372    1947 
   Size:        288     969     115     575

  Fragments arranged by size: 

                 969     575     288     115
 
PvuII CAG'CTG

Cuts at:      0    1602    1947 
   Size:       1602     345
 
SacI G_AGCT'C

Cuts at:      0    1946    1947 
   Size:       1946       1
 
SacII CC_GC'GG

Cuts at:      0     144    1947 
   Size:        144    1803
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1328    1947 
   Size:       1328     619
 
SfcI C'TryA_G

Cuts at:      0     284    1061    1104    1253    1368    1947 
   Size:        284     777      43     149     115     579

  Fragments arranged by size: 

                 777     579     284     149     115      43
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1604    1947 
   Size:       1604     343
 
SfoI GGC'GCC

Cuts at:      0      65    1947 
   Size:         65    1882
 
StyI C'CwwG_G

Cuts at:      0     114     893    1461    1706    1842    1947 
   Size:        114     779     568     245     136     105

  Fragments arranged by size: 

                 779     568     245     136     114     105
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     127    1211    1947 
   Size:        127    1084     736

  Fragments arranged by size: 

                1084     736     127
 
TatI w'GTAC_w

Cuts at:      0     317     929    1232    1947 
   Size:        317     612     303     715

  Fragments arranged by size: 

                 715     612     317     303
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     574     606     968    1000    1838    1870    1947 
   Size:        574      32     362      32     838      32      77

  Fragments arranged by size: 

                 838     574     362      77      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1080    1947 
   Size:       1080     867
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     600     835    1947 
   Size:        600     235    1112

  Fragments arranged by size: 

                1112     600     235
 

 Enzymes that do cut:

     AarI     AcuI     AloI    AlwNI     ApaI    ApaLI     ApoI     AvaI
    AvrII     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI
    BfrBI     BglI    BglII Bme1580I    BmgBI     BmrI     BpmI   Bpu10I
     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrDI    BsrFI
    BsrGI    BssSI   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI
    CspCI     DraI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I     FalI    HaeII    Hin4I     KasI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NsiI    PflMI     PfoI
     PpiI   PspOMI     PstI    PvuII     SacI    SacII     SapI     SfcI
     SfiI     SfoI     StyI    TaqII     TatI     TstI  Tth111I     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AscI     AseI    AsiSI     BaeI    BamHI
     BcgI     BlpI     BmtI     BplI    BpuEI    BsaAI     BsgI    BsiWI
    BspHI    BsrBI   BssHII   BstAPI    BstBI  BstZ17I    BtgZI     ClaI
   DraIII     DrdI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     NdeI     NheI
     NotI     NruI     NspI     PacI     PasI     PciI     PmeI     PmlI
    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SalI
    SanDI     SbfI     ScaI    SexAI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     XbaI
     XhoI     XmaI     XmnI     ZraI
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