Restriction map for pF1KB6167
##################################
# NO CUT ENZYMES (  153 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        Asp718I     AspEI       AspHI       AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BfrI        BglI        BlnI        
BlpI        BmyI        Bpu1102I    BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BseRI       BsiEI       
BsiHKAI     BsiWI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspEI       BspHI       BspLU11I    BsrBI       
BsrFI       BsrGI       BssHII      Bst1107I    BstBI       
BstEII      BstNI       BstPI       BstUI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      CpoI        
DraIII      EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRII      EcoRV       EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HhaI        HinP1I      
HincII      HindII      HpaI        KasI        KpnI        
Ksp632I     KspI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MspA1I      MunI        
MvaI        MvnI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AcyI            3  GGCGTC               G[GA]^CG[CT]C
AlwI         1494  GATCC                GGATC(4/5)
AlwNI        1003  CAGAATCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspI         1770  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII        1717  GGACC                G^G[AT]CC
BcnI          164  CCGGG                CC^[GC]GG
BsaHI           3  GGCGTC               G[GA]^CG[CT]C
BsmAI        1806  GTCTC                GTCTC(1/5)
BsmBI        1805  CGTCTC               CGTCTC(1/5)
BspDI        1936  ATCGAT               AT^CGAT
BsrDI         173  GCAATG               GCAATG(2/0)
BssKI         164  CCGGG                ^CC[ACGT]GG
BssSI        1613  CTCGTG               CACGAG(-5/-1)
BstAPI       1108  GCAACTGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        1197  CCAAGAGAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I       1717  GGACC                G^G[ACGT]CC
ClaI         1936  ATCGAT               AT^CGAT
DraII        1716  AGGACCT              [GA]G^G[ACGT]CC[CT]
DrdI         1208  GACGTGCAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I     1716  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRI        1013  GAATTC               G^AATTC
HaeIII        848  GGCC                 GG^CC
HapII         164  CCGG                 C^CGG
HgaI            4  GCGTC                GACGC(5/10)
Hin1I           3  GGCGTC               G[GA]^CG[CT]C
HindIII      1315  AAGCTT               A^AGCTT
HpaII         164  CCGG                 C^CGG
MspI          164  CCGG                 C^CGG
NciI          164  CCGGG                CC^[GC]GG
PflFI        1770  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        1716  AGGACCT              [GA]G^G[AT]CC[CT]
PstI         1476  CTGCAG               CTGCA^G
Sau96I       1717  GGACC                G^G[ACGT]CC
ScaI         1472  AGTACT               AGT^ACT
ScrFI         164  CCGGG                CC^[ACGT]GG
Tth111I      1770  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AciI          197  CCGC                 CCGC(-3/-1)
              726  GCGG                
AfaI         1248  GTAC                 GT^AC
             1473  GTAC                
AseI          441  ATTAAT               AT^TAAT
              922  ATTAAT              
AsnI          441  ATTAAT               AT^TAAT
              922  ATTAAT              
BbsI          433  GAAGAC               GAAGAC(2/6)
             1669  GAAGAC              
BbvI         1570  GCAGC                GCAGC(8/12)
             1879  GCTGC               
BfaI          491  CTAG                 C^TAG
              610  CTAG                
BglII        1482  AGATCT               A^GATCT
             1620  AGATCT              
BpmI          569  CTCCAG               CTGGAG(16/14)
             1624  CTGGAG              
BpuAI         433  GAAGAC               GAAGAC(2/6)
             1669  GAAGAC              
BsgI         1211  GTGCAG               GTGCAG(16/14)
             1568  GTGCAG              
BsmFI         681  GGGAC                GGGAC(10/14)
             1305  GGGAC               
Cac8I         169  GCAAGC               GC[ACGT]^[ACGT]GC
             1503  GCAGGC              
DraI          259  TTTAAA               TTT^AAA
              450  TTTAAA              
DsaI          220  CCATGG               C^C[GA][CT]GG
             1181  CCATGG              
EcoNI         340  CCTACAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1497  CCTGAAGCAGG         
EcoT14I       220  CCATGG               C^C[AT][AT]GG
             1181  CCATGG              
MaeI          491  CTAG                 C^TAG
              610  CTAG                
MslI         1018  CATGCAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1381  CATTTTGATG          
NcoI          220  CCATGG               C^CATGG
             1181  CCATGG              
NlaIV        1044  GGAGCC               GG[ACGT]^[ACGT]CC
             1599  GGCTCC              
PflMI         220  CCATGGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              438  CCAATTAATGG         
PleI          567  GACTC                GAGTC(4/5)
              976  GACTC               
PshBI         441  ATTAAT               AT^TAAT
              922  ATTAAT              
RsaI         1248  GTAC                 GT^AC
             1473  GTAC                
StyI          220  CCATGG               C^C[AT][AT]GG
             1181  CCATGG              
TseI         1570  GCAGC                G^C[AT]GC
             1879  GCTGC               
Tsp45I        565  GTGAC                ^GT[GC]AC
             1768  GTGAC               
Van91I        220  CCATGGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              438  CCAATTAATGG         


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AcsI        AluI        ApoI        Asp700I     BsaJI       
BsiYI       BslI        BspMI       BsrI        BstF5I      
BstYI       DdeI        DpnII       Fnu4HI      FokI        
HinfI       HphI        ItaI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MwoI        NdeII       NlaIII      NspI        Sau3AI      
SfaNI       SfcI        TaqI        TfiI        Tru9I       
Tsp509I     TspRI       TthHB8I     XhoII       XmnI        
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