Restriction map for pF1KB6167
 (Linear) (Six-base) MAPSORT of: KIBB6167.seq  Check: 4878  from: 1  to: 1947



KIBB6167.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     201    1012    1579    1947 
   Size:        201     811     567     368

  Fragments arranged by size: 

                 811     567     368     201
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     268     911    1519    1876    1947 
   Size:        268     643     608     357      71

  Fragments arranged by size: 

                 643     608     357     268      71
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1008    1947 
   Size:       1008     939
 
ApoI r'AATT_y

Cuts at:      0      65      71     464     800    1013    1091    1854    1947 
   Size:         65       6     393     336     213      78     763      93

  Fragments arranged by size: 

                 763     393     336     213      93      78      65       6
            
 
AseI AT'TA_AT

Cuts at:      0     442     923    1947 
   Size:        442     481    1024

  Fragments arranged by size: 

                1024     481     442
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1642    1675    1947 
   Size:       1642      33     272

  Fragments arranged by size: 

                1642     272      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     440    1676    1947 
   Size:        440    1236     271

  Fragments arranged by size: 

                1236     440     271
 
BglII A'GATC_T

Cuts at:      0    1482    1620    1947 
   Size:       1482     138     327

  Fragments arranged by size: 

                1482     327     138
 
BmgBI CAC'GTC

Cuts at:      0    1210    1947 
   Size:       1210     737
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     656    1947 
   Size:        656    1291
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     554    1645    1947 
   Size:        554    1091     302

  Fragments arranged by size: 

                1091     554     302
 
BsaHI Gr'CG_yC

Cuts at:      0       4    1947 
   Size:          4    1943
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1232    1589    1947 
   Size:       1232     357     358

  Fragments arranged by size: 

                1232     358     357
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1811    1947 
   Size:       1811     136
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     201    1012    1204    1579    1947 
   Size:        201     811     192     375     368

  Fragments arranged by size: 

                 811     375     368     201     192
 
BsrDI GCAATG_nn'

Cuts at:      0     180    1947 
   Size:        180    1767
 
BssSI C'ACGA_G

Cuts at:      0    1613    1947 
   Size:       1613     334
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1114    1947 
   Size:       1114     833
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1204    1947 
   Size:       1204     743
 
BstYI r'GATC_y

Cuts at:      0    1482    1493    1620    1947 
   Size:       1482      11     127     327

  Fragments arranged by size: 

                1482     327     127      11
 
BtgI C'CryG_G

Cuts at:      0     220    1181    1947 
   Size:        220     961     766

  Fragments arranged by size: 

                 961     766     220
 
BtsI GCAGTG_nn'

Cuts at:      0     148     663    1947 
   Size:        148     515    1284

  Fragments arranged by size: 

                1284     515     148
 
ClaI AT'CG_AT

Cuts at:      0    1937    1947 
   Size:       1937      10
 
DraI TTT'AAA

Cuts at:      0     261     452    1947 
   Size:        261     191    1495

  Fragments arranged by size: 

                1495     261     191
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1214    1947 
   Size:       1214     733
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     268     554     911    1519    1645    1876    1947 
   Size:        268     286     357     608     126     231      71

  Fragments arranged by size: 

                 608     357     286     268     231     126      71
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     344    1501    1947 
   Size:        344    1157     446

  Fragments arranged by size: 

                1157     446     344
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1717    1947 
   Size:       1717     230
 
EcoRI G'AATT_C

Cuts at:      0    1013    1947 
   Size:       1013     934
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1747    1947 
   Size:       1747     200
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     777     809    1315    1347    1472    1504    1947 
   Size:        777      32     506      32     125      32     443

  Fragments arranged by size: 

                 777     506     443     125      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0    1315    1947 
   Size:       1315     632
 
MslI CAynn'nnrTG

Cuts at:      0    1022    1385    1947 
   Size:       1022     363     562

  Fragments arranged by size: 

                1022     562     363
 
NcoI C'CATG_G

Cuts at:      0     220    1181    1947 
   Size:        220     961     766

  Fragments arranged by size: 

                 961     766     220
 
NspI r_CATG'y

Cuts at:      0      58     508    1412    1753    1890    1947 
   Size:         58     450     904     341     137      57

  Fragments arranged by size: 

                 904     450     341     137      58      57
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     226     444    1947 
   Size:        226     218    1503

  Fragments arranged by size: 

                1503     226     218
 
PpuMI rG'GwC_Cy

Cuts at:      0    1717    1947 
   Size:       1717     230
 
PsiI TTA'TAA

Cuts at:      0     704    1947 
   Size:        704    1243
 
PstI C_TGCA'G

Cuts at:      0    1480    1947 
   Size:       1480     467
 
ScaI AGT'ACT

Cuts at:      0    1474    1947 
   Size:       1474     473
 
SfcI C'TryA_G

Cuts at:      0     341     669    1476    1674    1947 
   Size:        341     328     807     198     273

  Fragments arranged by size: 

                 807     341     328     273     198
 
StyI C'CwwG_G

Cuts at:      0     220    1181    1947 
   Size:        220     961     766

  Fragments arranged by size: 

                 961     766     220
 
TatI w'GTAC_w

Cuts at:      0    1247    1472    1947 
   Size:       1247     225     475

  Fragments arranged by size: 

                1247     475     225
 
Tth111I GACn'n_nGTC

Cuts at:      0    1773    1947 
   Size:       1773     174
 
XmnI GAAnn'nnTTC

Cuts at:      0     215    1278    1789    1947 
   Size:        215    1063     511     158

  Fragments arranged by size: 

                1063     511     215     158
 

 Enzymes that do cut:

     AarI     AcuI    AlwNI     ApoI     AseI     BaeI     BbsI    BglII
    BmgBI     BplI     BpmI    BsaHI     BsgI    BsmBI    BspMI    BsrDI
    BssSI   BstAPI    BstXI    BstYI     BtgI     BtsI     ClaI     DraI
     DrdI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    Hin4I  HindIII
     MslI     NcoI     NspI    PflMI    PpuMI     PsiI     PstI     ScaI
     SfcI     StyI     TatI  Tth111I     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII    BamHI     BanI    BanII     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I     BmrI     BmtI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI
    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I    BspEI    BspHI
    BsrBI    BsrFI    BsrGI   BssHII    BstBI   BstEII  BstZ17I   Bsu36I
    BtgZI    CspCI   DraIII     EaeI     EagI     EarI     EciI  EcoICRI
    EcoRV     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TstI     XbaI     XcmI     XhoI     XmaI     ZraI
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