Restriction map for pF1KB7345
##################################
# NO CUT ENZYMES (   91 enzymes) #
##################################

AatII       AccII       AccIII      AcyI        AgeI        
AhdI        Alw44I      ApaI        ApaLI       AscI        
AspEI       AspHI       AspI        AviII       AvrII       
BalI        BbeI        BbrPI       BglI        BlnI        
BsaAI       BsaHI       BsaI        BseAI       BsiEI       
BsiHKAI     BsiWI       BsmBI       Bsp1407I    BspEI       
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Cfr10I      CpoI        EaeI        
EagI        Eam1105I    EclXI       Eco52I      EcoO65I     
EcoT22I     FseI        FspI        HgaI        Hin1I       
KasI        KspI        MluI        MluNI       MroI        
MscI        MvnI        NaeI        NarI        NdeI        
NgoMIV      NotI        NruI        NsiI        NspV        
PacI        PflFI       PinAI       PmaCI       PmeI        
PmlI        PvuI        RsrII       SacI        SacII       
SalI        SbfI        SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        Sse8387I    SspBI       
Tth111I     


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

Acc65I       4363  GGTACC               G^GTACC
AccI         1270  GTAGAC               GT^[AC][GT]AC
AclI         2486  AACGTT               AA^CGTT
AflIII       4911  ACATGT               A^C[GA][CT]GT
Aor51HI      2852  AGCGCT               AGC^GCT
Asp718I      4363  GGTACC               G^GTACC
BcgI         2369  CGAAAGTACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         4419  TGATCA               T^GATCA
BsaWI        5141  TCCGGT               [AT]^CCGG[AT]
BspDI        2834  ATCGAT               AT^CGAT
BspLU11I     4911  ACATGT               A^CATGT
ClaI         2834  ATCGAT               AT^CGAT
DraIII       1447  CACATTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III     2852  AGCGCT               AGC^GCT
EcoNI         529  CCTGCCAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1315  GAATTC               G^AATTC
EcoRV        2748  GATATC               GAT^ATC
FbaI         4419  TGATCA               T^GATCA
HaeII        2852  AGCGCT               [GA]GCGC^[CT]
HindIII      3341  AAGCTT               A^AGCTT
KpnI         4363  GGTACC               GGTAC^C
MfeI         5123  CAATTG               C^AATTG
MunI         5123  CAATTG               C^AATTG
NheI         2689  GCTAGC               G^CTAGC
PaeR7I       4298  CTCGAG               C^TCGAG
PshAI        1248  GACTTCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     2486  AACGTT               AA^CGTT
SapI         2824  GAAGAGC              GCTCTTC(1/4)
SmaI         5256  CCCGGG               CCC^GGG
SphI         1103  GCATGC               GCATG^C
XhoI         4298  CTCGAG               C^TCGAG
XmaCI        5256  CCCGGG               C^CCGGG
XmaI         5256  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AvaI         4298  CTCGAG               C^[CT]CG[GA]G
             5256  CCCGGG              
BbvCI         178  CCTCAGC              CCTCAGC(-5/-2)
              691  CCTCAGC             
BciVI        3061  GGATAC               GTATCC(6/5)
             3114  GTATCC              
BcnI         5256  CCCGG                CC^[GC]GG
             5257  CCGGG               
BglII         828  AGATCT               A^GATCT
             1377  AGATCT              
BsaBI        3436  GATAAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             5860  GATCTTAATC          
BsgI           71  CTGCAC               GTGCAG(16/14)
             5038  GTGCAG              
BsmI          722  GCATTC               GAATGC(1/-1)
             2109  GCATTC              
BsoBI        4298  CTCGAG               C^[CT]CG[GA]G
             5256  CCCGGG              
DrdI         3763  GACATCATGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4306  GACAAAGTTGTC        
HapII        5142  CCGG                 C^CGG
             5257  CCGG                
HpaI          318  GTTAAC               GTT^AAC
              793  GTTAAC              
HpaII        5142  CCGG                 C^CGG
             5257  CCGG                
MaeII        1719  ACGT                 A^CGT
             2487  ACGT                
MamI         3436  GATAAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             5860  GATCTTAATC          
MspI         5142  CCGG                 C^CGG
             5257  CCGG                
NciI         5256  CCCGG                CC^[GC]GG
             5257  CCGGG               
NcoI         1702  CCATGG               C^CATGG
             5098  CCATGG              
NspI         1103  GCATGC               [GA]CATG^[CT]
             4911  ACATGT              
PflMI         643  CCAGATGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1192  CCAGCTCCTGG         
ScaI          890  AGTACT               AGT^ACT
             2373  AGTACT              
SexAI        4174  ACCTGGT              A^CC[AT]GGT
             4583  ACCAGGT             
StuI           98  AGGCCT               AGG^CCT
             5220  AGGCCT              
SwaI         3221  ATTTAAAT             ATTT^AAAT
             4832  ATTTAAAT            
Van91I        643  CCAGATGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1192  CCAGCTCCTGG         
XbaI          831  TCTAGA               T^CTAGA
             5868  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (  108 enzymes) #
#########################################

AciI        AcsI        AfaI        AflII       AluI        
AlwI        AlwNI       AocI        ApoI        AseI        
AsnI        Asp700I     AvaII       BamHI       BanI        
BanII       BbsI        BbvI        BfaI        BfrI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BspHI       BspMI       BsrDI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstYI       Bsu36I      Cac8I       CelII       CfoI        
Cfr13I      DdeI        DpnII       DraI        DraII       
DsaI        EarI        Eco81I      EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HhaI        HinP1I      HincII      HindII      HinfI       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MvaI        MwoI        NdeII       
NlaIII      NlaIV       PleI        PpuMI       PshBI       
PstI        PvuII       RcaI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XcmI        XhoII       XmnI        
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