Restriction map for pF1KB7345
 (Linear) (Six-base) MAPSORT of: KIBB7345.seq  Check: 766  from: 1  to: 6669



KIBB7345.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    4449    6669 
   Size:       4449    2220
 
AccI GT'mk_AC

Cuts at:      0    1271    6669 
   Size:       1271    5398
 
Acc65I G'GTAC_C

Cuts at:      0    4363    6669 
   Size:       4363    2306
 
AclI AA'CG_TT

Cuts at:      0    2487    6669 
   Size:       2487    4182
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     177     737     802    1151    1235    1286    1391    1541 
   Size:        177     560      65     349      84      51     105     150

Cuts at:   1541    1840    2057    2365    3458    4214    4712    5537    6020 
   Size:        299     217     308    1093     756     498     825     483

Cuts at:   6020    6669 
   Size:        649

  Fragments arranged by size: 

                1093     825     756     649     560     498     483     349
                 308     299     217     177     150     105      84      65
                  51
 
AfeI AGC'GCT

Cuts at:      0    2854    6669 
   Size:       2854    3815
 
AflII C'TTAA_G

Cuts at:      0    2856    5356    6262    6669 
   Size:       2856    2500     906     407

  Fragments arranged by size: 

                2856    2500     906     407
 
AflIII A'CryG_T

Cuts at:      0    4911    6669 
   Size:       4911    1758
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1561    6074    6669 
   Size:       1561    4513     595

  Fragments arranged by size: 

                4513    1561     595
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1198    1885    2143    3631    4273    4526    6669 
   Size:       1198     687     258    1488     642     253    2143

  Fragments arranged by size: 

                2143    1488    1198     687     642     258     253
 
ApoI r'AATT_y

Cuts at:      0     388     409    1315    1620    1675    2028    2696    5982 
   Size:        388      21     906     305      55     353     668    3286

Cuts at:   5982    6029    6669 
   Size:         47     640

  Fragments arranged by size: 

                3286     906     668     640     388     353     305      55
                  47      21
 
AseI AT'TA_AT

Cuts at:      0     230    1115    6197    6669 
   Size:        230     885    5082     472

  Fragments arranged by size: 

                5082     885     472     230
 
AvaI C'yCGr_G

Cuts at:      0    4298    5256    6669 
   Size:       4298     958    1413

  Fragments arranged by size: 

                4298    1413     958
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    3305    3338    5111    5144    6669 
   Size:       3305      33    1773      33    1525

  Fragments arranged by size: 

                3305    1773    1525      33      33
 
BamHI G'GATC_C

Cuts at:      0    3441    3898    4752    6669 
   Size:       3441     457     854    1917

  Fragments arranged by size: 

                3441    1917     854     457
 
BanI G'GyrC_C

Cuts at:      0       3     652    3426    4171    4210    4363    5053    5260 
   Size:          3     649    2774     745      39     153     690     207

Cuts at:   5260    6669 
   Size:       1409

  Fragments arranged by size: 

                2774    1409     745     690     649     207     153      39
                   3
 
BanII G_rGCy'C

Cuts at:      0    3698    5278    6023    6669 
   Size:       3698    1580     745     646

  Fragments arranged by size: 

                3698    1580     745     646
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     461     614    4310    5914    6633    6669 
   Size:        461     153    3696    1604     719      36

  Fragments arranged by size: 

                3696    1604     719     461     153      36
 
BbvCI CC'TCA_GC

Cuts at:      0     179     692    6669 
   Size:        179     513    5977

  Fragments arranged by size: 

                5977     513     179
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2358    2392    6669 
   Size:       2358      34    4277

  Fragments arranged by size: 

                4277    2358      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    3055    3125    6669 
   Size:       3055      70    3544

  Fragments arranged by size: 

                3544    3055      70
 
BclI T'GATC_A

Cuts at:      0    4419    6669 
   Size:       4419    2250
 
BglII A'GATC_T

Cuts at:      0     828    1377    6669 
   Size:        828     549    5292

  Fragments arranged by size: 

                5292     828     549
 
BlpI GC'TnA_GC

Cuts at:      0     713    2186    2645    6669 
   Size:        713    1473     459    4024

  Fragments arranged by size: 

                4024    1473     713     459
 
Bme1580I G_kGCm'C

Cuts at:      0     657    1024    1331    1550    5256    6669 
   Size:        657     367     307     219    3706    1413

  Fragments arranged by size: 

                3706    1413     657     367     307     219
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     582    1385    3683    5273    6033    6669 
   Size:        582     803    2298    1590     760     636

  Fragments arranged by size: 

                2298    1590     803     760     636     582
 
BmtI G_CTAG'C

Cuts at:      0    2693    6669 
   Size:       2693    3976
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     205     385    2227    2364    4112    5171    6669 
   Size:        205     180    1842     137    1748    1059    1498

  Fragments arranged by size: 

                1842    1748    1498    1059     205     180     137
 
Bpu10I CC'TnA_GC

Cuts at:      0     179     692    6669 
   Size:        179     513    5977

  Fragments arranged by size: 

                5977     513     179
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     973    1788    1952    2794    5254    5446    5892    6283 
   Size:        973     815     164     842    2460     192     446     391

Cuts at:   6283    6669 
   Size:        386

  Fragments arranged by size: 

                2460     973     842     815     446     391     386     192
                 164
 
BsaBI GATnn'nnATC

Cuts at:      0    3440    5864    6669 
   Size:       3440    2424     805

  Fragments arranged by size: 

                3440    2424     805
 
BsaWI w'CCGG_w

Cuts at:      0    5141    6669 
   Size:       5141    1528
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      14      44    1639    1669    4134    4164    5979    6009 
   Size:         14      30    1595      30    2465      30    1815      30

Cuts at:   6009    6149    6179    6669 
   Size:        140      30     490

  Fragments arranged by size: 

                2465    1815    1595     490     140      30      30      30
                  30      30      14
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     617    2863    4115    5446    5449    6669 
   Size:        617    2246    1252    1331       3    1220

  Fragments arranged by size: 

                2246    1331    1252    1220     617       3
 
BseYI C'CCAG_C

Cuts at:      0    6349    6669 
   Size:       6349     320
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      56    5059    6669 
   Size:         56    5003    1610

  Fragments arranged by size: 

                5003    1610      56
 
BsmI GAATG_Cn'

Cuts at:      0     722    2109    6669 
   Size:        722    1387    4560

  Fragments arranged by size: 

                4560    1387     722
 
Bsp1286I G_dGCh'C

Cuts at:      0     657    1024    1331    1550    3698    5256    5278    6023 
   Size:        657     367     307     219    2148    1558      22     745

Cuts at:   6023    6669 
   Size:        646

  Fragments arranged by size: 

                2148    1558     745     657     646     367     307     219
                  22
 
BspHI T'CATG_A

Cuts at:      0    1503    1575    1734    1952    2177    2262    4715    6669 
   Size:       1503      72     159     218     225      85    2453    1954

  Fragments arranged by size: 

                2453    1954    1503     225     218     159      85      72
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1191    4449    4697    5337    6314    6669 
   Size:       1191    3258     248     640     977     355

  Fragments arranged by size: 

                3258    1191     977     640     355     248
 
BsrDI GCAATG_nn'

Cuts at:      0     104    2510    5451    6669 
   Size:        104    2406    2941    1218

  Fragments arranged by size: 

                2941    2406    1218     104
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1347    1650    3925    6190    6669 
   Size:       1347     303    2275    2265     479

  Fragments arranged by size: 

                2275    2265    1347     479     303
 
BstYI r'GATC_y

Cuts at:      0     253     828    1377    2076    2996    3358    3441    3898 
   Size:        253     575     549     699     920     362      83     457

Cuts at:   3898    4269    4752    6669 
   Size:        371     483    1917

  Fragments arranged by size: 

                1917     920     699     575     549     483     457     371
                 362     253      83
 
Bsu36I CC'TnA_GG

Cuts at:      0     557    3233    4757    6669 
   Size:        557    2676    1524    1912

  Fragments arranged by size: 

                2676    1912    1524     557
 
BtgI C'CryG_G

Cuts at:      0     161    1702    1903    5098    6669 
   Size:        161    1541     201    3195    1571

  Fragments arranged by size: 

                3195    1571    1541     201     161
 
BtsI GCAGTG_nn'

Cuts at:      0    1550    5960    6669 
   Size:       1550    4410     709

  Fragments arranged by size: 

                4410    1550     709
 
ClaI AT'CG_AT

Cuts at:      0    2835    6669 
   Size:       2835    3834
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    6420    6455    6669 
   Size:       6420      35     214

  Fragments arranged by size: 

                6420     214      35
 
DraI TTT'AAA

Cuts at:      0    2364    3224    4835    5736    6330    6669 
   Size:       2364     860    1611     901     594     339

  Fragments arranged by size: 

                2364    1611     901     860     594     339
 
DraIII CAC_nnn'GTG

Cuts at:      0    1452    6669 
   Size:       1452    5217
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    3769    4312    6669 
   Size:       3769     543    2357

  Fragments arranged by size: 

                3769    2357     543
 
EarI CTCTTCn'nnn_

Cuts at:      0     196    2819    2840    3008    3248    3272    3593    6669 
   Size:        196    2623      21     168     240      24     321    3076

  Fragments arranged by size: 

                3076    2623     321     240     196     168      24      21
            
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     177     205     385     737     802    1151    1235    1286 
   Size:        177      28     180     352      65     349      84      51

Cuts at:   1286    1391    1541    1840    2057    2227    2364    2365    3458 
   Size:        105     150     299     217     170     137       1    1093

Cuts at:   3458    4112    4214    4712    5171    5537    6020    6669 
   Size:        654     102     498     459     366     483     649

  Fragments arranged by size: 

                1093     654     649     498     483     459     366     352
                 349     299     217     180     177     170     150     137
                 105     102      84      65      51      28       1
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     533    6669 
   Size:        533    6136
 
EcoO109I rG'GnC_Cy

Cuts at:      0     174    1555    2385    4438    5191    6669 
   Size:        174    1381     830    2053     753    1478

  Fragments arranged by size: 

                2053    1478    1381     830     753     174
 
EcoRI G'AATT_C

Cuts at:      0    1315    6669 
   Size:       1315    5354
 
EcoRV GAT'ATC

Cuts at:      0    2750    6669 
   Size:       2750    3919
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     157     832    2395    3586    6669 
   Size:        157     675    1563    1191    3083

  Fragments arranged by size: 

                3083    1563    1191     675     157
 
HaeII r_GCGC'y

Cuts at:      0    2856    6669 
   Size:       2856    3813
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1639    1671    2739    2771    2926    2958    3501    3533 
   Size:       1639      32    1068      32     155      32     543      32

Cuts at:   3533    3924    3956    4335    4367    4849    4881    6669 
   Size:        391      32     379      32     482      32    1788

  Fragments arranged by size: 

                1788    1639    1068     543     482     391     379     155
                  32      32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0     320     795    3704    6669 
   Size:        320     475    2909    2965

  Fragments arranged by size: 

                2965    2909     475     320
 
HindIII A'AGCT_T

Cuts at:      0    3341    6669 
   Size:       3341    3328
 
HpaI GTT'AAC

Cuts at:      0     320     795    6669 
   Size:        320     475    5874

  Fragments arranged by size: 

                5874     475     320
 
KpnI G_GTAC'C

Cuts at:      0    4367    6669 
   Size:       4367    2302
 
MfeI C'AATT_G

Cuts at:      0    5123    6669 
   Size:       5123    1546
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     231    2511    3511    3872    6058    6135    6288    6420 
   Size:        231    2280    1000     361    2186      77     153     132

Cuts at:   6420    6669 
   Size:        249

  Fragments arranged by size: 

                2280    2186    1000     361     249     231     153     132
                  77
 
MslI CAynn'nnrTG

Cuts at:      0    1324    1957    2525    3224    4106    4358    5097    6278 
   Size:       1324     633     568     699     882     252     739    1181

Cuts at:   6278    6669 
   Size:        391

  Fragments arranged by size: 

                1324    1181     882     739     699     633     568     391
                 252
 
MspA1I CmG'CkG

Cuts at:      0     973    2157    4636    4695    5842    6394    6436    6669 
   Size:        973    1184    2479      59    1147     552      42     233

  Fragments arranged by size: 

                2479    1184    1147     973     552     233      59      42
            
 
NcoI C'CATG_G

Cuts at:      0    1702    5098    6669 
   Size:       1702    3396    1571

  Fragments arranged by size: 

                3396    1702    1571
 
NheI G'CTAG_C

Cuts at:      0    2689    6669 
   Size:       2689    3980
 
NspI r_CATG'y

Cuts at:      0    1107    4915    6669 
   Size:       1107    3808    1754

  Fragments arranged by size: 

                3808    1754    1107
 
PasI CC'CwG_GG

Cuts at:      0    6579    6669 
   Size:       6579      90
 
PciI A'CATG_T

Cuts at:      0    4911    6669 
   Size:       4911    1758
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     649    1198    6669 
   Size:        649     549    5471

  Fragments arranged by size: 

                5471     649     549
 
PfoI T'CCnGG_A

Cuts at:      0    1064    1197    5187    6669 
   Size:       1064     133    3990    1482

  Fragments arranged by size: 

                3990    1482    1064     133
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      47      79    6160    6192    6496    6528    6669 
   Size:         47      32    6081      32     304      32     141

  Fragments arranged by size: 

                6081     304     141      47      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     174    2385    4438    5191    6669 
   Size:        174    2211    2053     753    1478

  Fragments arranged by size: 

                2211    2053    1478     753     174
 
PshAI GACnn'nnGTC

Cuts at:      0    1252    6669 
   Size:       1252    5417
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    3620    3652    6669 
   Size:       3620      32    3017

  Fragments arranged by size: 

                3620    3017      32
 
PstI C_TGCA'G

Cuts at:      0     776    5348    5402    5946    6053    6325    6669 
   Size:        776    4572      54     544     107     272     344

  Fragments arranged by size: 

                4572     776     544     344     272     107      54
 
PvuII CAG'CTG

Cuts at:      0     973    2157    4636    4695    5842    6394    6436    6669 
   Size:        973    1184    2479      59    1147     552      42     233

  Fragments arranged by size: 

                2479    1184    1147     973     552     233      59      42
            
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2819    6669 
   Size:       2819    3850
 
ScaI AGT'ACT

Cuts at:      0     892    2375    6669 
   Size:        892    1483    4294

  Fragments arranged by size: 

                4294    1483     892
 
SexAI A'CCwGG_T

Cuts at:      0    4174    4583    6669 
   Size:       4174     409    2086

  Fragments arranged by size: 

                4174    2086     409
 
SfcI C'TryA_G

Cuts at:      0     284     772    1606    3725    5287    5344    5398    5942 
   Size:        284     488     834    2119    1562      57      54     544

Cuts at:   5942    6049    6321    6669 
   Size:        107     272     348

  Fragments arranged by size: 

                2119    1562     834     544     488     348     284     272
                 107      57      54
 
SmaI CCC'GGG

Cuts at:      0    5258    6669 
   Size:       5258    1411
 
SmlI C'TyrA_G

Cuts at:      0     952    1767    1967    2809    2856    4298    5269    5356 
   Size:        952     815     200     842      47    1442     971      87

Cuts at:   5356    5461    5907    6262    6298    6669 
   Size:        105     446     355      36     371

  Fragments arranged by size: 

                1442     971     952     842     815     446     371     355
                 200     105      87      47      36
 
SphI G_CATG'C

Cuts at:      0    1107    6669 
   Size:       1107    5562
 
SspI AAT'ATT

Cuts at:      0    1041    2503    3461    6669 
   Size:       1041    1462     958    3208

  Fragments arranged by size: 

                3208    1462    1041     958
 
StuI AGG'CCT

Cuts at:      0     100    5222    6669 
   Size:        100    5122    1447

  Fragments arranged by size: 

                5122    1447     100
 
StyI C'CwwG_G

Cuts at:      0     500    1702    5098    6455    6669 
   Size:        500    1202    3396    1357     214

  Fragments arranged by size: 

                3396    1357    1202     500     214
 
SwaI ATTT'AAAT

Cuts at:      0    3224    4835    6669 
   Size:       3224    1611    1834

  Fragments arranged by size: 

                3224    1834    1611
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      21     601    1404    2452    3602    6363    6669 
   Size:         21     580     803    1048    1150    2761     306

  Fragments arranged by size: 

                2761    1150    1048     803     580     306      21
 
TatI w'GTAC_w

Cuts at:      0     890     956    2373    4640    6669 
   Size:        890      66    1417    2267    2029

  Fragments arranged by size: 

                2267    2029    1417     890      66
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      14      46    1438    1470    1857    1889    5614    5646 
   Size:         14      32    1392      32     387      32    3725      32

Cuts at:   5646    6149    6181    6350    6382    6669 
   Size:        503      32     169      32     287

  Fragments arranged by size: 

                3725    1392     503     387     287     169      32      32
                  32      32      32      32      14
 
XbaI T'CTAG_A

Cuts at:      0     831    5868    6669 
   Size:        831    5037     801

  Fragments arranged by size: 

                5037     831     801
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     111    2283    2527    2646    6669 
   Size:        111    2172     244     119    4023

  Fragments arranged by size: 

                4023    2172     244     119     111
 
XhoI C'TCGA_G

Cuts at:      0    4298    6669 
   Size:       4298    2371
 
XmaI C'CCGG_G

Cuts at:      0    5256    6669 
   Size:       5256    1413
 
XmnI GAAnn'nnTTC

Cuts at:      0     812    1679    2783    4961    5162    5956    6669 
   Size:        812     867    1104    2178     201     794     713

  Fragments arranged by size: 

                2178    1104     867     812     794     713     201
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AlfI    AlwNI     ApoI     AseI     AvaI     BaeI    BamHI     BanI
    BanII     BbsI    BbvCI     BcgI    BciVI     BclI    BglII     BlpI
 Bme1580I     BmrI     BmtI     BpmI   Bpu10I    BpuEI    BsaBI    BsaWI
    BsaXI    BseRI    BseYI     BsgI     BsmI Bsp1286I    BspHI    BspMI
    BsrDI   BstAPI    BstYI   Bsu36I     BtgI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EarI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MmeI     MslI   MspA1I     NcoI     NheI     NspI     PasI
     PciI    PflMI     PfoI     PpiI    PpuMI    PshAI     PsrI     PstI
    PvuII     SapI     ScaI    SexAI     SfcI     SmaI     SmlI     SphI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI     XbaI
     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     AscI
    AsiSI    AvrII     BbeI    BfrBI     BglI    BmgBI     BplI     BsaI
    BsaAI    BsaHI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I
    BtgZI     EaeI     EagI     EciI  EcoICRI     FseI     FspI    FspAI
     KasI     MluI     MscI     NaeI     NarI     NdeI   NgoMIV     NotI
     NruI     NsiI     PacI     PmeI     PmlI     PsiI   PspOMI    PspXI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SrfI  Tth111I     ZraI
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