Restriction map for pF1KA0708
##################################
# NO CUT ENZYMES (   51 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        AscI        AseI        AsnI        Asp718I     
BciVI       BfrI        BsaBI       BseAI       BspDI       
BspEI       BssHII      Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     ClaI        DraI        DrdI        
EcoO65I     FseI        HpaI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MunI        
NruI        NspV        PacI        PinAI       PmeI        
PshBI       Psp1406I    PvuI        SacII       SfiI        
SfuI        SnaBI       SpeI        SspI        SwaI        
XbaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccII        6917  CGCG                 CG^CG
AhdI         6024  GACGGTGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI        6024  GACGGTGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1824  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        4577  TGCGCA               TGC^GCA
AvrII        1599  CCTAGG               C^CTAGG
BamHI        7241  GGATCC               G^GATCC
BbrPI         439  CACGTG               CAC^GTG
BcgI         3924  GCAGCTGTGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         5670  TGATCA               T^GATCA
BlnI         1599  CCTAGG               C^CTAGG
BsiWI        6192  CGTACG               C^GTACG
Bsp1407I     7052  TGTACA               T^GTACA
BsrGI        7052  TGTACA               T^GTACA
BstUI        6917  CGCG                 CG^CG
CpoI         4805  CGGACCG              CG^G[AT]CCG
DraIII       1904  CACAGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     6024  GACGGTGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I      3199  ATGCAT               ATGCA^T
FbaI         5670  TGATCA               T^GATCA
FspI         4577  TGCGCA               TGC^GCA
HindIII       294  AAGCTT               A^AGCTT
MseI         1925  TTAA                 T^TAA
MvnI         6917  CGCG                 CG^CG
NaeI         3370  GCCGGC               GCC^GGC
NdeI         6613  CATATG               CA^TATG
NgoMIV       3370  GCCGGC               G^CCGGC
NheI         6601  GCTAGC               G^CTAGC
NotI         2292  GCGGCCGC             GC^GGCCGC
NsiI         3199  ATGCAT               ATGCA^T
PflFI        1824  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         439  CACGTG               CAC^GTG
PmlI          439  CACGTG               CAC^GTG
PshAI        2355  GACTTCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII        4805  CGGACCG              CG^G[AT]CCG
SacI         3742  GAGCTC               GAGCT^C
SapI         2588  GCTCTTC              GCTCTTC(1/4)
SbfI         5974  CCTGCAGG             CCTGCA^GG
ScaI         4199  AGTACT               AGT^ACT
SgrAI        3997  CACCGGCG             C[GA]^CCGG[CT]G
Sse8387I     5974  CCTGCAGG             CCTGCA^GG
SspBI        7052  TGTACA               T^GTACA
Tru9I        1925  TTAA                 T^TAA
Tth111I      1824  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

Asp700I        70  GAACTCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3801  GAACCGCTTC          
BsaAI         439  CACGTG               [CT]AC^GT[GA]
             5812  TACGTG              
BsaWI        4364  ACCGGA               [AT]^CCGG[AT]
             6373  ACCGGA              
BspLU11I      518  ACATGT               A^CATGT
             3940  ACATGT              
EagI          416  CGGCCG               C^GGCCG
             2293  CGGCCG              
EclXI         416  CGGCCG               C^GGCCG
             2293  CGGCCG              
Eco52I        416  CGGCCG               C^GGCCG
             2293  CGGCCG              
EcoRI        1102  GAATTC               G^AATTC
             5167  GAATTC              
EcoRV        1475  GATATC               GAT^ATC
             2067  GATATC              
HincII       1263  GTCGAC               GT[CT]^[GA]AC
             6628  GTCGAC              
HindII       1263  GTCGAC               GT[CT]^[GA]AC
             6628  GTCGAC              
PaeR7I        302  CTCGAG               C^TCGAG
             4259  CTCGAG              
SalI         1263  GTCGAC               G^TCGAC
             6628  GTCGAC              
SmaI         2520  CCCGGG               CCC^GGG
             3863  CCCGGG              
XhoI          302  CTCGAG               C^TCGAG
             4259  CTCGAG              
XmaCI        2520  CCCGGG               C^CCGGG
             3863  CCCGGG              
XmaI         2520  CCCGGG               C^CCGGG
             3863  CCCGGG              
XmnI           70  GAACTCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3801  GAACCGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  145 enzymes) #
#########################################

AccI        AciI        AcsI        AcyI        AfaI        
AflIII      AluI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbeI        BbsI        BbvCI       BbvI        
BcnI        BfaI        BglI        BglII       BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaHI       
BsaI        BsaJI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspHI       
BspMI       BsrBI       BsrDI       BsrFI       BsrI        
BssKI       BssSI       BstAPI      BstF5I      BstNI       
BstXI       BstYI       Bsu36I      Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        Eco47III    
Eco81I      EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        KasI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MslI        MspA1I      MspI        MvaI        MwoI        
NarI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RcaI        RsaI        Sau3AI      
Sau96I      ScrFI       SexAI       SfaNI       SfcI        
SfoI        SmlI        SphI        StuI        StyI        
TaqI        TfiI        TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com