Restriction map for
pF1KB9140
(Linear) (Six-base) MAPSORT of: KIBB9140.seq Check: 443 from: 1 to: 1128
KIBB9140.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AflII C'TTAA_G
Cuts at: 0 261 1128
Size: 261 867
AlwNI CAG_nnn'CTG
Cuts at: 0 958 1128
Size: 958 170
ApoI r'AATT_y
Cuts at: 0 607 1128
Size: 607 521
AvaI C'yCGr_G
Cuts at: 0 128 1128
Size: 128 1000
BanI G'GyrC_C
Cuts at: 0 17 1128
Size: 17 1111
BanII G_rGCy'C
Cuts at: 0 36 118 761 1128
Size: 36 82 643 367
Fragments arranged by size:
643 367 82 36
BbeI G_GCGC'C
Cuts at: 0 21 1128
Size: 21 1107
BbsI GAAGACnn'nnnn_
Cuts at: 0 834 1046 1128
Size: 834 212 82
Fragments arranged by size:
834 212 82
BglII A'GATC_T
Cuts at: 0 507 1128
Size: 507 621
Bme1580I G_kGCm'C
Cuts at: 0 908 1128
Size: 908 220
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 20 917 1097 1128
Size: 20 897 180 31
Fragments arranged by size:
897 180 31 20
BsaI GGTCTCn'nnnn_
Cuts at: 0 146 1128
Size: 146 982
BsaHI Gr'CG_yC
Cuts at: 0 18 1128
Size: 18 1110
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 91 495 1128
Size: 91 404 633
Fragments arranged by size:
633 404 91
BseYI C'CCAG_C
Cuts at: 0 545 1128
Size: 545 583
BsiEI CG_ry'CG
Cuts at: 0 29 1128
Size: 29 1099
BsiHKAI G_wGCw'C
Cuts at: 0 36 118 761 1128
Size: 36 82 643 367
Fragments arranged by size:
643 367 82 36
Bsp1286I G_dGCh'C
Cuts at: 0 36 118 761 908 1128
Size: 36 82 643 147 220
Fragments arranged by size:
643 220 147 82 36
BspHI T'CATG_A
Cuts at: 0 87 761 1128
Size: 87 674 367
Fragments arranged by size:
674 367 87
BstYI r'GATC_y
Cuts at: 0 507 924 954 1128
Size: 507 417 30 174
Fragments arranged by size:
507 417 174 30
Bsu36I CC'TnA_GG
Cuts at: 0 554 1128
Size: 554 574
BtgI C'CryG_G
Cuts at: 0 71 241 1128
Size: 71 170 887
Fragments arranged by size:
887 170 71
BtsI GCAGTG_nn'
Cuts at: 0 255 1128
Size: 255 873
EarI CTCTTCn'nnn_
Cuts at: 0 104 731 1008 1128
Size: 104 627 277 120
Fragments arranged by size:
627 277 120 104
EcoICRI GAG'CTC
Cuts at: 0 34 116 759 1128
Size: 34 82 643 369
Fragments arranged by size:
643 369 82 34
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 20 917 1097 1128
Size: 20 897 180 31
Fragments arranged by size:
897 180 31 20
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 188 1128
Size: 188 940
FspI TGC'GCA
Cuts at: 0 475 1128
Size: 475 653
HaeII r_GCGC'y
Cuts at: 0 21 1128
Size: 21 1107
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 18 50 82 114 650 682 1128
Size: 18 32 32 32 536 32 446
Fragments arranged by size:
536 446 32 32 32 32 18
KasI G'GCGC_C
Cuts at: 0 17 1128
Size: 17 1111
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1111 1128
Size: 1111 17
MspA1I CmG'CkG
Cuts at: 0 53 1128
Size: 53 1075
NarI GG'CG_CC
Cuts at: 0 18 1128
Size: 18 1110
NcoI C'CATG_G
Cuts at: 0 71 1128
Size: 71 1057
NspI r_CATG'y
Cuts at: 0 887 1128
Size: 887 241
PspXI vC'TCGA_Gb
Cuts at: 0 128 1128
Size: 128 1000
PvuI CG_AT'CG
Cuts at: 0 29 1128
Size: 29 1099
PvuII CAG'CTG
Cuts at: 0 53 1128
Size: 53 1075
SacI G_AGCT'C
Cuts at: 0 36 118 761 1128
Size: 36 82 643 367
Fragments arranged by size:
643 367 82 36
SapI GCTCTTCn'nnn_
Cuts at: 0 731 1128
Size: 731 397
SfcI C'TryA_G
Cuts at: 0 306 1128
Size: 306 822
SfoI GGC'GCC
Cuts at: 0 19 1128
Size: 19 1109
SmlI C'TyrA_G
Cuts at: 0 128 261 1128
Size: 128 133 867
Fragments arranged by size:
867 133 128
SspI AAT'ATT
Cuts at: 0 1065 1128
Size: 1065 63
StyI C'CwwG_G
Cuts at: 0 71 1128
Size: 71 1057
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1019 1128
Size: 1019 109
Tth111I GACn'n_nGTC
Cuts at: 0 94 1034 1128
Size: 94 940 94
Fragments arranged by size:
940 94 94
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 78 1128
Size: 78 1050
XhoI C'TCGA_G
Cuts at: 0 128 1128
Size: 128 1000
XmnI GAAnn'nnTTC
Cuts at: 0 1053 1128
Size: 1053 75
Enzymes that do cut:
AflII AlwNI ApoI AvaI BanI BanII BbeI BbsI
BglII Bme1580I BpmI BsaI BsaHI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspHI BstYI Bsu36I BtgI BtsI EarI
EcoICRI Eco57MI FalI FspI HaeII Hin4I KasI MmeI
MspA1I NarI NcoI NspI PspXI PvuI PvuII SacI
SapI SfcI SfoI SmlI SspI StyI TaqII Tth111I
XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI
BclI BfrBI BglI BlpI BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI BsgI BsiWI
BsmI BsmBI BspEI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EaeI EagI EciI
EcoNI EcoO109I EcoRI EcoRV FseI FspAI HincII HindIII
HpaI KpnI MfeI MluI MscI MslI NaeI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI RsrII SacII SalI SanDI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI TatI TstI XbaI XmaI
ZraI