Restriction map for pF1KB5669
 (Linear) (Six-base) MAPSORT of: KIBB5669.seq  Check: 2551  from: 1  to: 1047



KIBB5669.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AccI GT'mk_AC

Cuts at:      0     590    1047 
   Size:        590     457
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     165     913    1047 
   Size:        165     748     134

  Fragments arranged by size: 

                 748     165     134
 
AflIII A'CryG_T

Cuts at:      0     503    1047 
   Size:        503     544
 
AlwNI CAG_nnn'CTG

Cuts at:      0     342    1047 
   Size:        342     705
 
ApoI r'AATT_y

Cuts at:      0     124     268     457     795    1047 
   Size:        124     144     189     338     252

  Fragments arranged by size: 

                 338     252     189     144     124
 
AseI AT'TA_AT

Cuts at:      0     227    1047 
   Size:        227     820
 
AvaI C'yCGr_G

Cuts at:      0     103    1047 
   Size:        103     944
 
BamHI G'GATC_C

Cuts at:      0       6     148    1047 
   Size:          6     142     899

  Fragments arranged by size: 

                 899     142       6
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     506    1047 
   Size:        506     541
 
BfrBI ATG'CAT

Cuts at:      0     966    1047 
   Size:        966      81
 
BglI GCCn_nnn'nGGC

Cuts at:      0      34    1047 
   Size:         34    1013
 
BlpI GC'TnA_GC

Cuts at:      0     257    1047 
   Size:        257     790
 
Bme1580I G_kGCm'C

Cuts at:      0    1015    1047 
   Size:       1015      32
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      92    1047 
   Size:         92     955
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76     150    1047 
   Size:         76      74     897

  Fragments arranged by size: 

                 897      76      74
 
BsaWI w'CCGG_w

Cuts at:      0     432     462    1047 
   Size:        432      30     585

  Fragments arranged by size: 

                 585     432      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      97     302    1047 
   Size:         97     205     745

  Fragments arranged by size: 

                 745     205      97
 
BsiEI CG_ry'CG

Cuts at:      0      70    1047 
   Size:         70     977
 
BsmI GAATG_Cn'

Cuts at:      0     929    1047 
   Size:        929     118
 
Bsp1286I G_dGCh'C

Cuts at:      0    1015    1047 
   Size:       1015      32
 
BsrFI r'CCGG_y

Cuts at:      0      28    1047 
   Size:         28    1019
 
BsrGI T'GTAC_A

Cuts at:      0     441    1047 
   Size:        441     606
 
BstBI TT'CG_AA

Cuts at:      0     921    1047 
   Size:        921     126
 
BstYI r'GATC_y

Cuts at:      0       6     148    1047 
   Size:          6     142     899

  Fragments arranged by size: 

                 899     142       6
 
Bsu36I CC'TnA_GG

Cuts at:      0     611    1047 
   Size:        611     436
 
BtgI C'CryG_G

Cuts at:      0      38      64    1047 
   Size:         38      26     983

  Fragments arranged by size: 

                 983      38      26
 
EaeI y'GGCC_r

Cuts at:      0      67     132    1047 
   Size:         67      65     915

  Fragments arranged by size: 

                 915      67      65
 
EagI C'GGCC_G

Cuts at:      0      67    1047 
   Size:         67     980
 
EarI CTCTTCn'nnn_

Cuts at:      0     304     999    1047 
   Size:        304     695      48

  Fragments arranged by size: 

                 695     304      48
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76     150     165     913    1047 
   Size:         76      74      15     748     134

  Fragments arranged by size: 

                 748     134      76      74      15
 
EcoRI G'AATT_C

Cuts at:      0     268    1047 
   Size:        268     779
 
HincII GTy'rAC

Cuts at:      0     730    1047 
   Size:        730     317
 
HindIII A'AGCT_T

Cuts at:      0     259     274    1047 
   Size:        259      15     773

  Fragments arranged by size: 

                 773     259      15
 
MscI TGG'CCA

Cuts at:      0     134    1047 
   Size:        134     913
 
MspA1I CmG'CkG

Cuts at:      0      66     543    1047 
   Size:         66     477     504

  Fragments arranged by size: 

                 504     477      66
 
NaeI GCC'GGC

Cuts at:      0      30    1047 
   Size:         30    1017
 
NdeI CA'TA_TG

Cuts at:      0     789    1047 
   Size:        789     258
 
NgoMIV G'CCGG_C

Cuts at:      0      28    1047 
   Size:         28    1019
 
NsiI A_TGCA'T

Cuts at:      0     968    1047 
   Size:        968      79
 
NspI r_CATG'y

Cuts at:      0     970    1047 
   Size:        970      77
 
PsiI TTA'TAA

Cuts at:      0     329    1047 
   Size:        329     718
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     284     316    1047 
   Size:        284      32     731

  Fragments arranged by size: 

                 731     284      32
 
PvuII CAG'CTG

Cuts at:      0     543    1047 
   Size:        543     504
 
SacII CC_GC'GG

Cuts at:      0      67    1047 
   Size:         67     980
 
SphI G_CATG'C

Cuts at:      0     970    1047 
   Size:        970      77
 
SspI AAT'ATT

Cuts at:      0     777    1047 
   Size:        777     270
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     926    1047 
   Size:        926     121
 
TatI w'GTAC_w

Cuts at:      0     441    1047 
   Size:        441     606
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    AlwNI     ApoI     AseI     AvaI    BamHI
     BbsI    BfrBI     BglI     BlpI Bme1580I     BmrI     BpmI    BsaWI
    BseRI    BsiEI     BsmI Bsp1286I    BsrFI    BsrGI    BstBI    BstYI
   Bsu36I     BtgI     EaeI     EagI     EarI  Eco57MI    EcoRI   HincII
  HindIII     MscI   MspA1I     NaeI     NdeI   NgoMIV     NsiI     NspI
     PsiI     PsrI    PvuII    SacII     SphI     SspI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI    AvrII
     BaeI     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI
    BglII    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaXI    BseYI     BsgI  BsiHKAI    BsiWI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI   BssHII    BssSI   BstAPI
   BstEII    BstXI  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MslI     NarI     NcoI     NheI     NotI
     NruI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI   PspOMI    PspXI     PstI     PvuI    RsrII
     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SrfI     StuI     StyI     SwaI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI
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