Restriction map for pF1KB5659
##################################
# NO CUT ENZYMES (  140 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BalI        BanI        BbeI        
BbrPI       BbsI        BbvCI       BclI        BfrI        
BglI        BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaWI       BseAI       BsiWI       
BsmBI       BsmI        BsoBI       BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DraIII      DrdI        DsaI        
Eam1105I    Eco47III    Eco81I      EcoNI       EcoO65I     
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HgaI        HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MunI        NarI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    StyI        SwaI        Tth111I     XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

AatII         365  GACGTC               GACGT^C
AcsI          499  GAATTC               [GA]^AATT[CT]
AcyI          365  GACGTC               G[GA]^CG[CT]C
AfaI          785  GTAC                 GT^AC
AlwNI         379  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          821  GGGCCC               GGGCC^C
ApoI          499  GAATTC               [GA]^AATT[CT]
AvaII          51  GGTCC                G^G[AT]CC
BamHI         259  GGATCC               G^GATCC
BbvI          425  GCAGC                GCAGC(8/12)
BcgI          241  CGAGATGCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         549  GTATCC               GTATCC(6/5)
BcnI          117  CCGGG                CC^[GC]GG
BfaI          445  CTAG                 C^TAG
BglII          86  AGATCT               A^GATCT
BsaHI         365  GACGTC               G[GA]^CG[CT]C
BsaI          733  GAGACC               GGTCTC(1/5)
BsgI          125  GTGCAG               GTGCAG(16/14)
BsmFI         582  GGGAC                GGGAC(10/14)
Bsp1407I      784  TGTACA               T^GTACA
BsrDI         168  GCAATG               GCAATG(2/0)
BsrFI         112  GCCGGC               [GA]^CCGG[CT]
BsrGI         784  TGTACA               T^GTACA
BstXI         403  CCAGATGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        112  GCCGGC               [GA]^CCGG[CT]
DraII         820  GGGGCCC              [GA]G^G[ACGT]CC[CT]
EaeI          114  CGGCCG               [CT]^GGCC[GA]
EagI          114  CGGCCG               C^GGCCG
EarI           64  GAAGAG               CTCTTC(1/4)
EclXI         114  CGGCCG               C^GGCCG
Eco52I        114  CGGCCG               C^GGCCG
EcoO109I      820  GGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoRI         499  GAATTC               G^AATTC
Fnu4HI        425  GCAGC                GC^[ACGT]GC
Hin1I         365  GACGTC               G[GA]^CG[CT]C
HphI          475  GGTGA                GGTGA(8/7)
ItaI          425  GCAGC                GC^[ACGT]GC
Ksp632I        64  GAAGAG               CTCTTC(1/4)
MaeI          445  CTAG                 C^TAG
MseI          458  TTAA                 T^TAA
MslI          238  CATCGAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          112  GCCGGC               GCC^GGC
NciI          117  CCGGG                CC^[GC]GG
NgoMIV        112  GCCGGC               G^CCGGC
NlaIII        792  CATG                 CATG^
PflMI         378  CCAGTGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII         887  CAGCTG               CAG^CTG
RsaI          785  GTAC                 GT^AC
SapI           64  GAAGAGC              GCTCTTC(1/4)
SfcI          432  CTACAG               C^T[GA][CT]AG
SmlI          655  CTCAAG               C^T[CT][GA]AG
SspBI         784  TGTACA               T^GTACA
SspI          538  AATATT               AAT^ATT
StuI          187  AGGCCT               AGG^CCT
TaqI          240  TCGA                 T^CGA
Tru9I         458  TTAA                 T^TAA
TseI          425  GCAGC                G^C[AT]GC
TthHB8I       240  TCGA                 T^CGA
Van91I        378  CCAGTGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AlwI          260  GATCC                GGATC(4/5)
              259  GGATC               
BpmI          492  CTCCAG               CTGGAG(16/14)
              703  CTGGAG              
BseRI         838  GAGGAG               GAGGAG(10/8)
              841  GAGGAG              
BsiEI          41  CGGTCG               CG[GA][CT]^CG
              114  CGGCCG              
BsmAI         733  GAGAC                GTCTC(1/5)
              155  GTCTC               
BstF5I        375  CATCC                GGATG(2/0)
              708  GGATG               
BstYI          86  AGATCT               [GA]^GATC[CT]
              259  GGATCC              
FokI          375  CATCC                GGATG(9/13)
              708  GGATG               
HapII         113  CCGG                 C^CGG
              117  CCGG                
HpaII         113  CCGG                 C^CGG
              117  CCGG                
MflI           86  AGATCT               [GA]^GATC[CT]
              259  GGATCC              
MspA1I        753  CCGCTG               C[AC]G^C[GT]G
              887  CAGCTG              
MspI          113  CCGG                 C^CGG
              117  CCGG                
PleI          490  GACTC                GAGTC(4/5)
              584  GACTC               
SacI          358  GAGCTC               GAGCT^C
              865  GAGCTC              
TfiI          478  GATTC                G^A[AT]TC
              810  GAATC               
Tsp45I        130  GTCAC                ^GT[GC]AC
              800  GTGAC               
Tsp509I       500  AATT                 ^AATT
              775  AATT                
XhoII          86  AGATCT               [GA]^GATC[CT]
              259  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AccII       AciI        AluI        AspHI       BanII       
BmyI        BsaJI       BsiHKAI     BsiYI       BslI        
Bsp1286I    BsrI        BssKI       BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
EcoRII      HaeIII      HhaI        HinP1I      HinfI       
MaeII       MaeIII      MboI        MboII       MnlI        
MvaI        MvnI        MwoI        NdeII       NlaIV       
Sau3AI      Sau96I      ScrFI       SfaNI       TspRI       
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