Restriction map for
pF1KB5646
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspI AviII AvrII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlnI BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I ClaI
CpoI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO109I EcoO65I EcoRI EcoT22I FbaI
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MslI MunI NaeI NarI
NcoI NgoMIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 94 CGCG CG^CG
AflII 714 CTTAAG C^TTAAG
AflIII 330 ACGTGT A^C[GA][CT]GT
AocI 1411 CCTCAGG CC^T[ACGT]AGG
Asp700I 385 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 67 GTGCTC G[AT]GC[AT]^C
BalI 1117 TGGCCA TGG^CCA
BamHI 1050 GGATCC G^GATCC
BanI 462 GGTGCC G^G[CT][GA]CC
BanII 222 GAGCCC G[GA]GC[CT]^C
BfrI 714 CTTAAG C^TTAAG
BlpI 69 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 69 GCTCAGC GC^T[ACGT]AGC
BsiHKAI 67 GTGCTC G[AT]GC[AT]^C
Bsp1407I 813 TGTACA T^GTACA
BsrBI 10 GAGCGG CCGCTC(-3/-3)
BsrFI 1430 GCCGGT [GA]^CCGG[CT]
BsrGI 813 TGTACA T^GTACA
BssHII 93 GCGCGC G^CGCGC
BstF5I 1128 GGATG GGATG(2/0)
BstUI 94 CGCG CG^CG
Bsu36I 1411 CCTCAGG CC^T[ACGT]AGG
CelII 69 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1430 GCCGGT [GA]^CCGG[CT]
DraI 324 TTTAAA TTT^AAA
EaeI 1117 TGGCCA [CT]^GGCC[GA]
Eco81I 1411 CCTCAGG CC^T[ACGT]AGG
EcoRV 492 GATATC GAT^ATC
EcoT14I 575 CCAAGG C^C[AT][AT]GG
FauI 23 CCCGC CCCGC(4/6)
FokI 1128 GGATG GGATG(9/13)
MaeII 330 ACGT A^CGT
MluNI 1117 TGGCCA TGG^CCA
MscI 1117 TGGCCA TGG^CCA
MspA1I 72 CAGCGG C[AC]G^C[GT]G
MvnI 94 CGCG CG^CG
NdeI 470 CATATG CA^TATG
NheI 375 GCTAGC G^CTAGC
PflMI 280 CCATTAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 114 CTGCAG CTGCA^G
SapI 119 GAAGAGC GCTCTTC(1/4)
SfcI 114 CTGCAG C^T[GA][CT]AG
SspBI 813 TGTACA T^GTACA
StuI 1279 AGGCCT AGG^CCT
StyI 575 CCAAGG C^C[AT][AT]GG
Van91I 280 CCATTAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1232 CCAAAAATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 385 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AseI 628 ATTAAT AT^TAAT
921 ATTAAT
AsnI 628 ATTAAT AT^TAAT
921 ATTAAT
AvaII 14 GGTCC G^G[AT]CC
76 GGTCC
BmyI 67 GTGCTC G[AGT]GC[ACT]^C
222 GAGCCC
BseRI 1316 CTCCTC GAGGAG(10/8)
1084 GAGGAG
BsmAI 290 GAGAC GTCTC(1/5)
349 GAGAC
BsmFI 15 GTCCC GGGAC(10/14)
77 GTCCC
Bsp1286I 67 GTGCTC G[AGT]GC[ACT]^C
222 GAGCCC
BstYI 1050 GGATCC [GA]^GATC[CT]
1198 GGATCT
CfoI 93 GCGC GCG^C
95 GCGC
EarI 119 GAAGAG CTCTTC(1/4)
722 GAAGAG
EcoNI 592 CCTGTCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1411 CCTCAGGAAGG
HhaI 93 GCGC GCG^C
95 GCGC
HinP1I 93 GCGC G^CGC
95 GCGC
HphI 1189 GGTGA GGTGA(8/7)
1245 GGTGA
Ksp632I 119 GAAGAG CTCTTC(1/4)
722 GAAGAG
MflI 1050 GGATCC [GA]^GATC[CT]
1198 GGATCT
PshBI 628 ATTAAT AT^TAAT
921 ATTAAT
TaqI 1149 TCGA T^CGA
1293 TCGA
Tsp45I 501 GTCAC ^GT[GC]AC
928 GTCAC
TthHB8I 1149 TCGA T^CGA
1293 TCGA
XhoII 1050 GGATCC [GA]^GATC[CT]
1198 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaI BbvI BcnI BfaI
BpmI BsaJI BsgI BsiYI BslI
BsoBI BsrI BssKI BstNI Cac8I
Cfr13I DdeI DpnII EcoRII Fnu4HI
HaeIII HapII HinfI HpaII ItaI
MaeI MaeIII MboI MboII MnlI
MseI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SmaI SmlI
TfiI Tru9I TseI Tsp509I TspRI
XmaCI XmaI