Restriction map for pF1KB5635
##################################
# NO CUT ENZYMES (  140 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BamHI       
BanII       BbeI        BbrPI       BbvCI       BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaHI       BsaI        BseAI       BsiEI       
BsiHKAI     BsmAI       BsmBI       BsoBI       Bsp1407I    
BspDI       BspEI       BspLU11I    BspMI       BsrFI       
BsrGI       BssHII      Bst1107I    BstAPI      BstEII      
BstPI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraII       DraIII      DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     FseI        FspI        HaeII       HgaI        
Hin1I       HindIII     KasI        KpnI        KspI        
MluI        MroI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RsrII       SacI        
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfoI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        Tth111I     XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccII        1110  CGCG                 CG^CG
AvaII         241  GGACC                G^G[AT]CC
BalI          231  TGGCCA               TGG^CCA
BanI          263  GGTGCC               G^G[CT][GA]CC
BbsI          694  GTCTTC               GAAGAC(2/6)
BcgI          749  CGAAAAATATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1083  GTATCC               GTATCC(6/5)
BclI         1265  TGATCA               T^GATCA
BcnI           81  CCGGG                CC^[GC]GG
BfaI          791  CTAG                 C^TAG
BmyI          264  GTGCCC               G[AGT]GC[ACT]^C
BpmI         1114  CTGGAG               CTGGAG(16/14)
BpuAI         694  GTCTTC               GAAGAC(2/6)
BsaBI        1266  GATCAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         548  ACCGGA               [AT]^CCGG[AT]
BseRI         164  GAGGAG               GAGGAG(10/8)
BsiWI         899  CGTACG               C^GTACG
BsmFI         969  GGGAC                GGGAC(10/14)
Bsp1286I      264  GTGCCC               G[AGT]GC[ACT]^C
BsrBI         724  GAGCGG               CCGCTC(-3/-3)
BssSI          89  CTCGTG               CACGAG(-5/-1)
BstBI        1132  TTCGAA               TT^CGAA
BstUI        1110  CGCG                 CG^CG
BstXI         671  CCATCTTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI         1111  GCGC                 GCG^C
DraI         1332  TTTAAA               TTT^AAA
DrdI          304  GACTGCACGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         937  CCTCAGAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I       514  ATGCAT               ATGCA^T
FbaI         1265  TGATCA               T^GATCA
HhaI         1111  GCGC                 GCG^C
HinP1I       1111  GCGC                 G^CGC
HincII        979  GTTAAC               GT[CT]^[GA]AC
HindII        979  GTTAAC               GT[CT]^[GA]AC
HpaI          979  GTTAAC               GTT^AAC
MaeI          791  CTAG                 C^TAG
MaeII         902  ACGT                 A^CGT
MamI         1266  GATCAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          767  CAATTG               C^AATTG
MluNI         231  TGGCCA               TGG^CCA
MscI          231  TGGCCA               TGG^CCA
MslI          309  CACGGTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI          767  CAATTG               C^AATTG
MvnI         1110  CGCG                 CG^CG
NciI           81  CCGGG                CC^[GC]GG
NsiI          514  ATGCAT               ATGCA^T
NspV         1132  TTCGAA               TT^CGAA
PflMI         331  CCAGAAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII         730  CAGCTG               CAG^CTG
SapI          772  GCTCTTC              GCTCTTC(1/4)
SfcI          221  CTACAG               C^T[GA][CT]AG
SfuI         1132  TTCGAA               TT^CGAA
SmlI         1204  CTCAAG               C^T[CT][GA]AG
Van91I        331  CCAGAAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

BsmI         1143  GAATGC               GAATGC(1/-1)
              516  GCATTC              
BspHI         777  TCATGA               T^CATGA
             1014  TCATGA              
BstYI         326  AGATCC               [GA]^GATC[CT]
             1200  GGATCT              
EarI          773  CTCTTC               CTCTTC(1/4)
             1123  GAAGAG              
FauI          425  CCCGC                CCCGC(4/6)
             1096  GCGGG               
HapII          81  CCGG                 C^CGG
              549  CCGG                
HpaII          81  CCGG                 C^CGG
              549  CCGG                
Ksp632I       773  CTCTTC               CTCTTC(1/4)
             1123  GAAGAG              
MflI          326  AGATCC               [GA]^GATC[CT]
             1200  GGATCT              
MseI          980  TTAA                 T^TAA
             1333  TTAA                
MspI           81  CCGG                 C^CGG
              549  CCGG                
NspI           33  GCATGT               [GA]CATG^[CT]
              956  ACATGC              
RcaI          777  TCATGA               T^CATGA
             1014  TCATGA              
TaqI          201  TCGA                 T^CGA
             1133  TCGA                
Tru9I         980  TTAA                 T^TAA
             1333  TTAA                
Tsp45I         94  GTGAC                ^GT[GC]AC
              857  GTGAC               
Tsp509I       204  AATT                 ^AATT
              768  AATT                
TthHB8I       201  TCGA                 T^CGA
             1133  TCGA                
XhoII         326  AGATCC               [GA]^GATC[CT]
             1200  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   45 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        BbvI        
BsaJI       BsgI        BsiYI       BslI        BsrDI       
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       EaeI        EcoRII      
Fnu4HI      FokI        HaeIII      HinfI       HphI        
ItaI        MaeIII      MboI        MboII       MnlI        
MspA1I      MvaI        MwoI        NdeII       NlaIII      
NlaIV       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       TfiI        TseI        TspRI       
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