Restriction map for pF1KB5559
##################################
# NO CUT ENZYMES (  186 enzymes) #
##################################

AatII       AccII       AccIII      AciI        AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     AspEI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BanII       BbeI        BbrPI       
BbsI        BbvCI       BbvI        BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaI        BsaJI       BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiWI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrFI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
Fnu4HI      FseI        FspI        HaeII       HapII       
HhaI        HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       ItaI        KasI        Ksp632I     
KspI        MaeI        MfeI        MluI        MluNI       
MroI        MscI        MspA1I      MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        Sau96I      SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TseI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

Acc65I        202  GGTACC               G^GTACC
AccI          430  GTAGAC               GT^[AC][GT]AC
AcsI          641  GAATTT               [GA]^AATT[CT]
AcyI            3  GGCGTC               G[GA]^CG[CT]C
ApoI          641  GAATTT               [GA]^AATT[CT]
Asp718I       202  GGTACC               G^GTACC
AspHI         187  GTGCTC               G[AT]GC[AT]^C
BpmI          136  CTGGAG               CTGGAG(16/14)
BsaBI         672  GATTGGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI           3  GGCGTC               G[GA]^CG[CT]C
BsiHKAI       187  GTGCTC               G[AT]GC[AT]^C
BsiYI           8  CCCCGTCTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI            8  CCCCGTCTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI          11  CGTCTC               CGTCTC(1/5)
BspMI         244  ACCTGC               ACCTGC(4/8)
BssKI         135  CCTGG                ^CC[ACGT]GG
BssSI         701  CTCGTG               CACGAG(-5/-1)
BstF5I        263  GGATG                GGATG(2/0)
BstNI         135  CCTGG                CC^[AT]GG
BstXI         666  CCAACTGATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         437  AGATCC               [GA]^GATC[CT]
Cac8I         658  GCTTGC               GC[ACGT]^[ACGT]GC
DraI          156  TTTAAA               TTT^AAA
EcoRII        135  CCTGG                ^CC[AT]GG
FokI          263  GGATG                GGATG(9/13)
HaeIII        168  GGCC                 GG^CC
HgaI            4  GCGTC                GACGC(5/10)
Hin1I           3  GGCGTC               G[GA]^CG[CT]C
KpnI          202  GGTACC               GGTAC^C
MaeII         448  ACGT                 A^CGT
MaeIII        466  GTCAC                ^GT[ACGT]AC
MamI          672  GATTGGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII         541  GAAGA                GAAGA(8/7)
MflI          437  AGATCC               [GA]^GATC[CT]
MnlI          696  CCTC                 CCTC(7/6)
MslI          454  CATTGTTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI          135  CCTGG                CC^[AT]GG
PstI           22  CTGCAG               CTGCA^G
ScrFI         135  CCTGG                CC^[ACGT]GG
TaqI          308  TCGA                 T^CGA
TfiI          677  GAATC                G^A[AT]TC
Tsp45I        466  GTCAC                ^GT[GC]AC
TspRI          90  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       308  TCGA                 T^CGA
XhoII         437  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AfaI          203  GTAC                 GT^AC
              548  GTAC                
AluI          252  AGCT                 AG^CT
              361  AGCT                
AlwI          438  GATCC                GGATC(4/5)
               86  GGATC               
BanI          202  GGTACC               G^G[CT][GA]CC
              223  GGCACC              
BmyI          187  GTGCTC               G[AGT]GC[ACT]^C
              222  GGGCAC              
BsmAI          18  GAGAC                GTCTC(1/5)
               12  GTCTC               
BsmFI           6  GTCCC                GGGAC(10/14)
              178  GTCCC               
Bsp1286I      187  GTGCTC               G[AGT]GC[ACT]^C
              222  GGGCAC              
BsrDI         192  CATTGC               GCAATG(2/0)
              280  GCAATG              
DdeI          504  CTCAG                C^T[ACGT]AG
              521  CTAAG               
DpnII          87  GATC                 ^GATC
              438  GATC                
MboI           87  GATC                 ^GATC
              438  GATC                
MwoI          116  GCATTTGGGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              125  GCAAGGTGAGC         
NdeII          87  GATC                 ^GATC
              438  GATC                
NlaIV         202  GGTACC               GG[ACGT]^[ACGT]CC
              223  GGCACC              
RsaI          203  GTAC                 GT^AC
              548  GTAC                
Sau3AI         87  GATC                 ^GATC
              438  GATC                
SfaNI         264  GATGC                GCATC(5/9)
              562  GATGC               
SfcI           22  CTGCAG               C^T[GA][CT]AG
              404  CTACAG              


#########################################
# 3 OR MORE CUT ENZYMES (    8 enzymes) #
#########################################

BsrI        HinfI       HphI        MseI        NlaIII      
PleI        Tru9I       Tsp509I     
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