Restriction map for pF1KB5490
##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AocI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp718I     AspI        AvaI        AviII       BalI        
BbeI        BbrPI       BbvCI       BcgI        BclI        
BfrI        BglI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsiEI       BsiWI       BsmAI       BsmBI       BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BsrBI       
BsrFI       BsrGI       BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstUI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraI        DrdI        DsaI        EaeI        EagI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       HhaI        Hin1I       HinP1I      
HincII      HindII      HpaI        KasI        KpnI        
KspI        MaeII       MamI        MluI        MluNI       
MroI        MscI        MspA1I      MvnI        NaeI        
NarI        NcoI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        SwaI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        


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# ONE CUT ENZYMES (   28 enzymes) #
###################################

AciI          402  GCGG                 CCGC(-3/-1)
Alw44I       1082  GTGCAC               G^TGCAC
ApaLI        1082  GTGCAC               G^TGCAC
Asp700I      1276  GAACGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII        1114  CCTAGG               C^CTAGG
BamHI        1197  GGATCC               G^GATCC
BanI         1219  GGTGCC               G^G[CT][GA]CC
BanII        1339  GGGCTC               G[GA]GC[CT]^C
BciVI         416  GTATCC               GTATCC(6/5)
BlnI         1114  CCTAGG               C^CTAGG
BpmI          554  CTCCAG               CTGGAG(16/14)
BsiYI         803  CCAGTGTTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          803  CCAGTGTTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspMI         903  GCAGGT               ACCTGC(4/8)
BsrDI          66  CATTGC               GCAATG(2/0)
BssSI        1180  CTCGTG               CACGAG(-5/-1)
BstAPI        664  GCAGCAAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         774  CCAGAGAATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII       1242  CACTGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI          745  GAAGAG               CTCTTC(1/4)
FauI          402  GCGGG                CCCGC(4/6)
HgaI          341  GACGC                GACGC(5/10)
HindIII       329  AAGCTT               A^AGCTT
Ksp632I       745  GAAGAG               CTCTTC(1/4)
MfeI         1382  CAATTG               C^AATTG
MunI         1382  CAATTG               C^AATTG
ScaI          531  AGTACT               AGT^ACT
XmnI         1276  GAACGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AflIII         55  ACATGT               A^C[GA][CT]GT
             1253  ACATGT              
AhdI         1165  GACCAAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1322  GACGGCGTGTC         
AspEI        1165  GACCAAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1322  GACGGCGTGTC         
BglII          17  AGATCT               A^GATCT
              312  AGATCT              
BsaJI        1114  CCTAGG               C^C[ACGT][ACGT]GG
             1193  CCAAGG              
BseRI         930  GAGGAG               GAGGAG(10/8)
              933  GAGGAG              
BsgI           32  GTGCAG               GTGCAG(16/14)
              574  GTGCAG              
BsmFI        1250  GGGAC                GGGAC(10/14)
             1173  GTCCC               
BsmI         1009  GCATTC               GAATGC(1/-1)
             1280  GCATTC              
BspLU11I       55  ACATGT               A^CATGT
             1253  ACATGT              
Eam1105I     1165  GACCAAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1322  GACGGCGTGTC         
EcoRI         135  GAATTC               G^AATTC
             1409  GAATTC              
EcoT14I      1114  CCTAGG               C^C[AT][AT]GG
             1193  CCAAGG              
PpuMI          48  AGGACCC              [GA]G^G[AT]CC[CT]
              509  AGGACCT             
SspI          220  AATATT               AAT^ATT
             1064  AATATT              
StyI         1114  CCTAGG               C^C[AT][AT]GG
             1193  CCAAGG              
TaqI          192  TCGA                 T^CGA
             1316  TCGA                
Tsp45I        376  GTGAC                ^GT[GC]AC
             1183  GTGAC               
TthHB8I       192  TCGA                 T^CGA
             1316  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        AlwNI       
ApoI        AspHI       AvaII       BbsI        BbvI        
BcnI        BfaI        BmyI        BpuAI       BsiHKAI     
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       EcoO109I    EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspI        
MvaI        MwoI        NciI        NdeI        NdeII       
NlaIII      NlaIV       NspI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       TfiI        Tru9I       
TseI        Tsp509I     TspRI       XhoII       
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