Restriction map for pF1KB5490
 (Linear) (Six-base) MAPSORT of: KIBB5490.seq  Check: 1090  from: 1  to: 1482



KIBB5490.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     337     629    1482 
   Size:        337     292     853

  Fragments arranged by size: 

                 853     337     292
 
AflIII A'CryG_T

Cuts at:      0      55    1253    1482 
   Size:         55    1198     229

  Fragments arranged by size: 

                1198     229      55
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1170    1327    1482 
   Size:       1170     157     155

  Fragments arranged by size: 

                1170     157     155
 
AlwNI CAG_nnn'CTG

Cuts at:      0     468     582     736     979    1482 
   Size:        468     114     154     243     503

  Fragments arranged by size: 

                 503     468     243     154     114
 
ApaLI G'TGCA_C

Cuts at:      0    1082    1482 
   Size:       1082     400
 
ApoI r'AATT_y

Cuts at:      0     135     456     846     873    1409    1433    1482 
   Size:        135     321     390      27     536      24      49

  Fragments arranged by size: 

                 536     390     321     135      49      27      24
 
AvrII C'CTAG_G

Cuts at:      0    1114    1482 
   Size:       1114     368
 
BamHI G'GATC_C

Cuts at:      0    1197    1482 
   Size:       1197     285
 
BanI G'GyrC_C

Cuts at:      0    1219    1482 
   Size:       1219     263
 
BanII G_rGCy'C

Cuts at:      0    1343    1482 
   Size:       1343     139
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     635    1391    1441    1482 
   Size:        635     756      50      41

  Fragments arranged by size: 

                 756     635      50      41
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     427    1482 
   Size:        427    1055
 
BglII A'GATC_T

Cuts at:      0      17     312    1482 
   Size:         17     295    1170

  Fragments arranged by size: 

                1170     295      17
 
Bme1580I G_kGCm'C

Cuts at:      0    1086    1224    1482 
   Size:       1086     138     258

  Fragments arranged by size: 

                1086     258     138
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     612    1482 
   Size:        612     870
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     539    1482 
   Size:        539     943
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     725     755    1482 
   Size:        725      30     727

  Fragments arranged by size: 

                 727     725      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     945     948    1482 
   Size:        945       3     534

  Fragments arranged by size: 

                 945     534       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      53     595    1482 
   Size:         53     542     887

  Fragments arranged by size: 

                 887     542      53
 
BsiHKAI G_wGCw'C

Cuts at:      0     602     717    1086    1482 
   Size:        602     115     369     396

  Fragments arranged by size: 

                 602     396     369     115
 
BsmI GAATG_Cn'

Cuts at:      0    1009    1280    1482 
   Size:       1009     271     202

  Fragments arranged by size: 

                1009     271     202
 
Bsp1286I G_dGCh'C

Cuts at:      0     602     717    1086    1224    1343    1482 
   Size:        602     115     369     138     119     139

  Fragments arranged by size: 

                 602     369     139     138     119     115
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     894    1482 
   Size:        894     588
 
BsrDI GCAATG_nn'

Cuts at:      0      65    1482 
   Size:         65    1417
 
BssSI C'ACGA_G

Cuts at:      0    1180    1482 
   Size:       1180     302
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     670    1482 
   Size:        670     812
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     781    1482 
   Size:        781     701
 
BstYI r'GATC_y

Cuts at:      0      17     312     620    1134    1197    1455    1482 
   Size:         17     295     308     514      63     258      27

  Fragments arranged by size: 

                 514     308     295     258      63      27      17
 
DraIII CAC_nnn'GTG

Cuts at:      0    1247    1482 
   Size:       1247     235
 
EarI CTCTTCn'nnn_

Cuts at:      0     740    1482 
   Size:        740     742
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     337     539     629    1482 
   Size:        337     202      90     853

  Fragments arranged by size: 

                 853     337     202      90
 
EcoO109I rG'GnC_Cy

Cuts at:      0      49     510     579    1482 
   Size:         49     461      69     903

  Fragments arranged by size: 

                 903     461      69      49
 
EcoRI G'AATT_C

Cuts at:      0     135    1409    1482 
   Size:        135    1274      73

  Fragments arranged by size: 

                1274     135      73
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     265    1058    1323    1482 
   Size:        265     793     265     159

  Fragments arranged by size: 

                 793     265     265     159
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     605     637    1156    1188    1313    1345    1482 
   Size:        605      32     519      32     125      32     137

  Fragments arranged by size: 

                 605     519     137     125      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0     329    1482 
   Size:        329    1153
 
MfeI C'AATT_G

Cuts at:      0    1382    1482 
   Size:       1382     100
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      24     298     497    1482 
   Size:         24     274     199     985

  Fragments arranged by size: 

                 985     274     199      24
 
MslI CAynn'nnrTG

Cuts at:      0     913    1097    1148    1482 
   Size:        913     184      51     334

  Fragments arranged by size: 

                 913     334     184      51
 
NdeI CA'TA_TG

Cuts at:      0      74    1203    1416    1482 
   Size:         74    1129     213      66

  Fragments arranged by size: 

                1129     213      74      66
 
NspI r_CATG'y

Cuts at:      0      59    1214    1257    1482 
   Size:         59    1155      43     225

  Fragments arranged by size: 

                1155     225      59      43
 
PciI A'CATG_T

Cuts at:      0      55    1253    1482 
   Size:         55    1198     229

  Fragments arranged by size: 

                1198     229      55
 
PfoI T'CCnGG_A

Cuts at:      0     946    1331    1482 
   Size:        946     385     151

  Fragments arranged by size: 

                 946     385     151
 
PpuMI rG'GwC_Cy

Cuts at:      0      49     510    1482 
   Size:         49     461     972

  Fragments arranged by size: 

                 972     461      49
 
ScaI AGT'ACT

Cuts at:      0     533    1482 
   Size:        533     949
 
SspI AAT'ATT

Cuts at:      0     222    1066    1482 
   Size:        222     844     416

  Fragments arranged by size: 

                 844     416     222
 
StyI C'CwwG_G

Cuts at:      0    1114    1193    1482 
   Size:       1114      79     289

  Fragments arranged by size: 

                1114     289      79
 
TatI w'GTAC_w

Cuts at:      0     531    1482 
   Size:        531     951
 
XmnI GAAnn'nnTTC

Cuts at:      0    1280    1482 
   Size:       1280     202
 

 Enzymes that do cut:

     AcuI   AflIII     AhdI    AlwNI    ApaLI     ApoI    AvrII    BamHI
     BanI    BanII     BbsI    BciVI    BglII Bme1580I     BmrI     BpmI
    BsaXI    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI
    BssSI   BstAPI    BstXI    BstYI   DraIII     EarI  Eco57MI EcoO109I
    EcoRI     FalI    Hin4I  HindIII     MfeI     MmeI     MslI     NdeI
     NspI     PciI     PfoI    PpuMI     ScaI     SspI     StyI     TatI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AleI     AlfI     AloI     ApaI     AscI     AseI    AsiSI     AvaI
     BaeI     BbeI    BbvCI     BcgI     BclI    BfrBI     BglI     BlpI
    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseYI    BsiEI    BsiWI    BsmBI    BspEI    BspHI
    BsrBI    BsrFI    BsrGI   BssHII    BstBI   BstEII  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI    EcoRV     FseI     FspI    FspAI
    HaeII   HincII     HpaI     KasI     KpnI     MluI     MscI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI
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