Restriction map for pF1KB5464
##################################
# NO CUT ENZYMES (  142 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AflII       
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     AspEI       AvaI        AviII       
AvrII       BamHI       BbeI        BbrPI       BbsI        
BbvCI       BciVI       BcnI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaI        BsaWI       BseAI       BsgI        BsiEI       
BsiWI       BsmAI       BsmBI       BsmI        BsoBI       
BspDI       BspEI       BspLU11I    BsrDI       BsrFI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FauI        
FseI        FspI        HapII       HincII      HindII      
HindIII     HpaI        HpaII       KasI        KspI        
MluI        MroI        MspI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    StuI        StyI        
SwaI        Van91I      XbaI        XhoI        XmaCI       
XmaI        XmnI        


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# ONE CUT ENZYMES (   66 enzymes) #
###################################

Acc65I        132  GGTACC               G^GTACC
AccII         102  CGCG                 CG^CG
AcyI           94  GACGCC               G[GA]^CG[CT]C
AflIII        254  ACGTGT               A^C[GA][CT]GT
AluI          324  AGCT                 AG^CT
Asp718I       132  GGTACC               G^GTACC
AspHI         601  GAGCAC               G[AT]GC[AT]^C
AvaII         117  GGACC                G^G[AT]CC
BalI          141  TGGCCA               TGG^CCA
BanI          132  GGTACC               G^G[CT][GA]CC
BbvI          325  GCTGC                GCAGC(8/12)
BcgI          737  GCAATTGTTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          636  TGATCA               T^GATCA
BglII         471  AGATCT               A^GATCT
BpmI          541  CTGGAG               CTGGAG(16/14)
BsaBI         415  GATAGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI          94  GACGCC               G[GA]^CG[CT]C
BsiHKAI       601  GAGCAC               G[AT]GC[AT]^C
BsmFI         400  GTCCC                GGGAC(10/14)
Bsp1407I      224  TGTACA               T^GTACA
BspMI         196  GCAGGT               ACCTGC(4/8)
BsrGI         224  TGTACA               T^GTACA
BstF5I         84  CATCC                GGATG(2/0)
BstUI         102  CGCG                 CG^CG
BstYI         471  AGATCT               [GA]^GATC[CT]
CfoI           76  GCGC                 GCG^C
DdeI          581  CTCAG                C^T[ACGT]AG
DraII         116  AGGACCC              [GA]G^G[ACGT]CC[CT]
DsaI          776  CCGTGG               C^C[GA][CT]GG
EaeI          141  TGGCCA               [CT]^GGCC[GA]
EarI          149  GAAGAG               CTCTTC(1/4)
EcoO109I      116  AGGACCC              [GA]G^G[ACGT]CC[CT]
FbaI          636  TGATCA               T^GATCA
FokI           84  CATCC                GGATG(9/13)
HaeII          75  GGCGCT               [GA]GCGC^[CT]
HgaI           94  GACGC                GACGC(5/10)
HhaI           76  GCGC                 GCG^C
Hin1I          94  GACGCC               G[GA]^CG[CT]C
HinP1I         76  GCGC                 G^CGC
HinfI         594  GATTC                G^A[ACGT]TC
KpnI          132  GGTACC               GGTAC^C
Ksp632I       149  GAAGAG               CTCTTC(1/4)
MamI          415  GATAGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          471  AGATCT               [GA]^GATC[CT]
MluNI         141  TGGCCA               TGG^CCA
MscI          141  TGGCCA               TGG^CCA
MslI          516  CATTGTTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        323  CAGCTG               C[AC]G^C[GT]G
MvnI          102  CGCG                 CG^CG
MwoI          319  GCCCCAGCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI          167  GCATGT               [GA]CATG^[CT]
PpuMI         116  AGGACCC              [GA]G^G[AT]CC[CT]
PstI          311  CTGCAG               CTGCA^G
PvuII         323  CAGCTG               CAG^CTG
SapI          149  GAAGAGC              GCTCTTC(1/4)
SfaNI         316  GATGC                GCATC(5/9)
SfcI          311  CTGCAG               C^T[GA][CT]AG
SspBI         224  TGTACA               T^GTACA
TaqI          660  TCGA                 T^CGA
TfiI          594  GATTC                G^A[AT]TC
TseI          325  GCTGC                G^C[AT]GC
Tsp45I         10  GTGAC                ^GT[GC]AC
TspRI         390  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       660  TCGA                 T^CGA
XcmI          530  CCAGAAACCTACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         471  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AcsI          382  GAATTT               [GA]^AATT[CT]
              769  AAATTT              
AlwI          421  GATCC                GGATC(4/5)
              498  GATCC               
ApoI          382  GAATTT               [GA]^AATT[CT]
              769  AAATTT              
AspI           94  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              662  GACTGTGTC           
BanII          55  GAGCCC               G[GA]GC[CT]^C
              304  GAGCCC              
BfaI          181  CTAG                 C^TAG
              208  CTAG                
BsaJI         642  CCTGGG               C^C[ACGT][ACGT]GG
              776  CCGTGG              
BseRI          70  GAGGAG               GAGGAG(10/8)
              212  GAGGAG              
BsrBI          23  CCGCTC               CCGCTC(-3/-3)
               63  GAGCGG              
BsrI          540  ACTGG                ACTGG(1/-1)
              136  CCAGT               
Cac8I         192  GCTTGC               GC[ACGT]^[ACGT]GC
              733  GCCAGC              
Cfr13I         30  GGCCC                G^G[ACGT]CC
              117  GGACC               
Fnu4HI         65  GCGGC                GC^[ACGT]GC
              325  GCTGC               
ItaI           65  GCGGC                GC^[ACGT]GC
              325  GCTGC               
MaeI          181  CTAG                 C^TAG
              208  CTAG                
MaeIII         10  GTGAC                ^GT[ACGT]AC
              546  GTTAC               
MfeI          341  CAATTG               C^AATTG
              738  CAATTG              
MunI          341  CAATTG               C^AATTG
              738  CAATTG              
PflFI          94  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              662  GACTGTGTC           
Sau96I         30  GGCCC                G^G[ACGT]CC
              117  GGACC               
SspI          230  AATATT               AAT^ATT
              352  AATATT              
Tth111I        94  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              662  GACTGTGTC           


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AciI        AfaI        BmyI        BsiYI       BslI        
Bsp1286I    BspHI       BssKI       BstNI       DpnII       
EcoRII      HaeIII      HphI        MaeII       MboI        
MboII       MnlI        MseI        MvaI        NdeII       
NlaIII      NlaIV       RcaI        RsaI        Sau3AI      
ScrFI       Tru9I       Tsp509I     
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