Restriction map for pF1KB5464
 (Linear) (Six-base) MAPSORT of: KIBB5464.seq  Check: 3038  from: 1  to: 798



KIBB5464.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     187     798 
   Size:        187     611
 
Acc65I G'GTAC_C

Cuts at:      0     132     798 
   Size:        132     666
 
AflIII A'CryG_T

Cuts at:      0     254     798 
   Size:        254     544
 
ApoI r'AATT_y

Cuts at:      0     382     769     798 
   Size:        382     387      29

  Fragments arranged by size: 

                 387     382      29
 
BanI G'GyrC_C

Cuts at:      0     132     798 
   Size:        132     666
 
BanII G_rGCy'C

Cuts at:      0      59     308     798 
   Size:         59     249     490

  Fragments arranged by size: 

                 490     249      59
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     726     760     798 
   Size:        726      34      38

  Fragments arranged by size: 

                 726      38      34
 
BclI T'GATC_A

Cuts at:      0     636     798 
   Size:        636     162
 
BglII A'GATC_T

Cuts at:      0     471     798 
   Size:        471     327
 
BmgBI CAC'GTC

Cuts at:      0     255     798 
   Size:        255     543
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     562     798 
   Size:        562     236
 
BsaBI GATnn'nnATC

Cuts at:      0     419     798 
   Size:        419     379
 
BsaHI Gr'CG_yC

Cuts at:      0      95     798 
   Size:         95     703
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      85     227     798 
   Size:         85     142     571

  Fragments arranged by size: 

                 571     142      85
 
BseYI C'CCAG_C

Cuts at:      0     321     798 
   Size:        321     477
 
BsiHKAI G_wGCw'C

Cuts at:      0     605     798 
   Size:        605     193
 
Bsp1286I G_dGCh'C

Cuts at:      0      59     308     605     798 
   Size:         59     249     297     193

  Fragments arranged by size: 

                 297     249     193      59
 
BspHI T'CATG_A

Cuts at:      0     438     597     669     798 
   Size:        438     159      72     129

  Fragments arranged by size: 

                 438     159     129      72
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     187     798 
   Size:        187     611
 
BsrBI CCG'CTC

Cuts at:      0      25      65     798 
   Size:         25      40     733

  Fragments arranged by size: 

                 733      40      25
 
BsrGI T'GTAC_A

Cuts at:      0     224     798 
   Size:        224     574
 
BstYI r'GATC_y

Cuts at:      0     471     798 
   Size:        471     327
 
BtgI C'CryG_G

Cuts at:      0     776     798 
   Size:        776      22
 
EaeI y'GGCC_r

Cuts at:      0     141     798 
   Size:        141     657
 
EarI CTCTTCn'nnn_

Cuts at:      0     144     798 
   Size:        144     654
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     562     798 
   Size:        562     236
 
EcoO109I rG'GnC_Cy

Cuts at:      0     117     798 
   Size:        117     681
 
HaeII r_GCGC'y

Cuts at:      0      79     798 
   Size:         79     719
 
KpnI G_GTAC'C

Cuts at:      0     136     798 
   Size:        136     662
 
MfeI C'AATT_G

Cuts at:      0     341     738     798 
   Size:        341     397      60

  Fragments arranged by size: 

                 397     341      60
 
MscI TGG'CCA

Cuts at:      0     143     798 
   Size:        143     655
 
MslI CAynn'nnrTG

Cuts at:      0     520     798 
   Size:        520     278
 
MspA1I CmG'CkG

Cuts at:      0     325     798 
   Size:        325     473
 
NspI r_CATG'y

Cuts at:      0     171     798 
   Size:        171     627
 
PpuMI rG'GwC_Cy

Cuts at:      0     117     798 
   Size:        117     681
 
PstI C_TGCA'G

Cuts at:      0     315     798 
   Size:        315     483
 
PvuII CAG'CTG

Cuts at:      0     325     798 
   Size:        325     473
 
SapI GCTCTTCn'nnn_

Cuts at:      0     144     798 
   Size:        144     654
 
SfcI C'TryA_G

Cuts at:      0     311     798 
   Size:        311     487
 
SspI AAT'ATT

Cuts at:      0     232     354     798 
   Size:        232     122     444

  Fragments arranged by size: 

                 444     232     122
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     543     798 
   Size:        543     255
 
TatI w'GTAC_w

Cuts at:      0     224     257     275     798 
   Size:        224      33      18     523

  Fragments arranged by size: 

                 523     224      33      18
 
Tth111I GACn'n_nGTC

Cuts at:      0      97     665     798 
   Size:         97     568     133

  Fragments arranged by size: 

                 568     133      97
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     537     798 
   Size:        537     261
 

 Enzymes that do cut:

     AarI   Acc65I   AflIII     ApoI     BanI    BanII     BcgI     BclI
    BglII    BmgBI     BpmI    BsaBI    BsaHI    BseRI    BseYI  BsiHKAI
 Bsp1286I    BspHI    BspMI    BsrBI    BsrGI    BstYI     BtgI     EaeI
     EarI  Eco57MI EcoO109I    HaeII     KpnI     MfeI     MscI     MslI
   MspA1I     NspI    PpuMI     PstI    PvuII     SapI     SfcI     SspI
    TaqII     TatI  Tth111I     XcmI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AcuI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
    BciVI    BfrBI     BglI     BlpI Bme1580I     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaWI    BsaXI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BsrDI    BsrFI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I
   HincII  HindIII     HpaI     KasI     MluI     MmeI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     StyI     SwaI     TstI     XbaI     XhoI     XmaI     XmnI     ZraI
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