Restriction map for pF1KB5443
##################################
# NO CUT ENZYMES (  144 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbsI        BbvCI       
BciVI       BfrI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaBI       BsaI        BseRI       
BsgI        BsiWI       BsmFI       BsmI        Bsp1407I    
BspDI       BspHI       BspLU11I    BspMI       BsrDI       
BsrGI       BsrI        BssHII      Bst1107I    BstAPI      
BstBI       BstF5I      BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DdeI        DraI        DraII       
DraIII      DrdI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoRI       EcoRV       EcoT22I     FokI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KasI        KpnI        Ksp632I     MamI        
MboII       MfeI        MluI        MluNI       MscI        
MunI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmeI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SalI        SapI        
SbfI        SfiI        SfoI        SfuI        SgrAI       
SnaBI       SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tsp509I     Tth111I     Van91I      
XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

AccI          361  GTCTAC               GT^[AC][GT]AC
AccIII        556  TCCGGA               T^CCGGA
AcyI            7  GGCGTC               G[GA]^CG[CT]C
AlwI          329  GGATC                GGATC(4/5)
ApaI           48  GGGCCC               GGGCC^C
AspHI         204  GAGCAC               G[AT]GC[AT]^C
AvaI          446  CCCGGG               C^[CT]CG[GA]G
BbrPI         349  CACGTG               CAC^GTG
BcgI          305  GCACTTTCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          245  TGATCA               T^GATCA
BfaI          268  CTAG                 C^TAG
BglI          178  GCCAAGGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI         349  CACGTG               [CT]AC^GT[GA]
BsaHI           7  GGCGTC               G[GA]^CG[CT]C
BsaWI         556  TCCGGA               [AT]^CCGG[AT]
BseAI         556  TCCGGA               T^CCGGA
BsiEI         492  CGACCG               CG[GA][CT]^CG
BsiHKAI       204  GAGCAC               G[AT]GC[AT]^C
BsmAI          70  GAGAC                GTCTC(1/5)
BsmBI          70  GAGACG               CGTCTC(1/5)
BsoBI         446  CCCGGG               C^[CT]CG[GA]G
BspEI         556  TCCGGA               T^CCGGA
BsrBI         514  GAGCGG               CCGCTC(-3/-3)
BsrFI         408  GCCGGT               [GA]^CCGG[CT]
BssSI         214  CACGAG               CACGAG(-5/-1)
BstEII        430  GGTGACC              G^GT[ACGT]ACC
BstPI         430  GGTGACC              G^GT[ACGT]ACC
BstXI          80  CCATCGTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         329  GGATCT               [GA]^GATC[CT]
Cfr10I        408  GCCGGT               [GA]^CCGG[CT]
EaeI          406  TGGCCG               [CT]^GGCC[GA]
EcoO65I       430  GGTGACC              G^GT[ACGT]ACC
EcoT14I       179  CCAAGG               C^C[AT][AT]GG
FbaI          245  TGATCA               T^GATCA
HaeII         175  AGCGCC               [GA]GCGC^[CT]
HgaI            8  GCGTC                GACGC(5/10)
Hin1I           7  GGCGTC               G[GA]^CG[CT]C
KspI           57  CCGCGG               CCGC^GG
MaeI          268  CTAG                 C^TAG
MflI          329  GGATCT               [GA]^GATC[CT]
MroI          556  TCCGGA               T^CCGGA
MseI          341  TTAA                 T^TAA
MspA1I         57  CCGCGG               C[AC]G^C[GT]G
PmaCI         349  CACGTG               CAC^GTG
PmlI          349  CACGTG               CAC^GTG
SacII          57  CCGCGG               CCGC^GG
ScaI          542  AGTACT               AGT^ACT
SexAI         260  ACCAGGT              A^CC[AT]GGT
SfaNI         155  GCATC                GCATC(5/9)
SfcI          333  CTACAG               C^T[GA][CT]AG
SmaI          446  CCCGGG               CCC^GGG
SmlI          505  CTCAAG               C^T[CT][GA]AG
SpeI          267  ACTAGT               A^CTAGT
StyI          179  CCAAGG               C^C[AT][AT]GG
Tru9I         341  TTAA                 T^TAA
TspRI         144  CACTG                CA[GC]TG[ACGT][ACGT]^
XhoII         329  GGATCT               [GA]^GATC[CT]
XmaCI         446  CCCGGG               C^CCGGG
XmaI          446  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AccII          58  CGCG                 CG^CG
              320  CGCG                
AfaI          265  GTAC                 GT^AC
              543  GTAC                
AluI           17  AGCT                 AG^CT
              509  AGCT                
BanII          48  GGGCCC               G[GA]GC[CT]^C
               76  GAGCCC              
BstUI          58  CGCG                 CG^CG
              320  CGCG                
DpnII         246  GATC                 ^GATC
              330  GATC                
DsaI           57  CCGCGG               C^C[GA][CT]GG
              137  CCACGG              
MaeIII        431  GTGAC                ^GT[ACGT]AC
              442  GTGAC               
MboI          246  GATC                 ^GATC
              330  GATC                
MslI          156  CATCTCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              378  CATACAGGTG          
MvnI           58  CGCG                 CG^CG
              320  CGCG                
NdeII         246  GATC                 ^GATC
              330  GATC                
NlaIII        243  CATG                 CATG^
              393  CATG                
NlaIV          48  GGGCCC               GG[ACGT]^[ACGT]CC
               75  GGAGCC              
PleI          115  GACTC                GAGTC(4/5)
              467  GAGTC               
RsaI          265  GTAC                 GT^AC
              543  GTAC                
Sau3AI        246  GATC                 ^GATC
              330  GATC                
TaqI          257  TCGA                 T^CGA
              547  TCGA                
TfiI          292  GATTC                G^A[AT]TC
              560  GATTC               
Tsp45I        431  GTGAC                ^GT[GC]AC
              442  GTGAC               
TthHB8I       257  TCGA                 T^CGA
              547  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AciI        BbvI        BcnI        BmyI        BpmI        
BsaJI       BsiYI       BslI        Bsp1286I    BssKI       
BstNI       Cac8I       CfoI        Cfr13I      EcoRII      
FauI        Fnu4HI      HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeII       MnlI        MspI        MvaI        MwoI        
NciI        Sau96I      ScrFI       TseI        
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