Restriction map for pF1KB5443
 (Circular) (Six-base) MAPSORT of: pF1KB5443.seq  Check: 2623  from: 1  to: 3692



pF1KB5443  3692 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AccI GT'mk_AC

Cuts at:    470     730     470 
   Size:        260    3432
 
Acc65I G'GTAC_C

Cuts at:    708     708 
   Size:       3692
 
AclI AA'CG_TT

Cuts at:   3532    3532 
   Size:       3692
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1517    1949    2696    2964    1517 
   Size:        432     747     268    2245

  Fragments arranged by size: 

                2245     747     432     268
 
AflIII A'CryG_T

Cuts at:   1044    2148    3125    1044 
   Size:       1104     977    1611

  Fragments arranged by size: 

                1611    1104     977
 
AgeI A'CCGG_T

Cuts at:   2080    2080 
   Size:       3692
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    286     286 
   Size:       3692
 
AlwNI CAG_nnn'CTG

Cuts at:    997    2564     997 
   Size:       1567    2125
 
ApaI G_GGCC'C

Cuts at:    160     160 
   Size:       3692
 
ApaLI G'TGCA_C

Cuts at:   2462    2462 
   Size:       3692
 
ApoI r'AATT_y

Cuts at:    696    3045    3301     696 
   Size:       2349     256    1087

  Fragments arranged by size: 

                2349    1087     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3692
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3692
 
AvaI C'yCGr_G

Cuts at:    554     712     554 
   Size:        158    3534
 
BamHI G'GATC_C

Cuts at:    717     717 
   Size:       3692
 
BanI G'GyrC_C

Cuts at:    708    1122    1370    1405     708 
   Size:        414     248      35    2995

  Fragments arranged by size: 

                2995     414     248      35
 
BanII G_rGCy'C

Cuts at:    160     188     706    1736     160 
   Size:         28     518    1030    2116

  Fragments arranged by size: 

                2116    1030     518      28
 
BbeI G_GCGC'C

Cuts at:   1126    1374    1126 
   Size:        248    3444
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124     402     436      90 
   Size:         34     278      34    3346

  Fragments arranged by size: 

                3346     278      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   1590    2351    3692    1590 
   Size:        761    1341    1590

  Fragments arranged by size: 

                1590    1341     761
 
BclI T'GATC_A

Cuts at:    353    1212     353 
   Size:        859    2833
 
BglI GCCn_nnn'nGGC

Cuts at:    292     292 
   Size:       3692
 
BglII A'GATC_T

Cuts at:   1207    3244    1207 
   Size:       2037    1655
 
BlpI GC'TnA_GC

Cuts at:    804     804 
   Size:       3692
 
Bme1580I G_kGCm'C

Cuts at:    160     253    1317    1410    2466     160 
   Size:         93    1064      93    1056    1386

  Fragments arranged by size: 

                1386    1064    1056      93      93
 
BmgBI CAC'GTC

Cuts at:    236     549     236 
   Size:        313    3379
 
BmrI ACTGGGnnnn_n'

Cuts at:    904     985    1320    3464     904 
   Size:         81     335    2144    1132

  Fragments arranged by size: 

                2144    1132     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    628    2059     628 
   Size:       1431    2261
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    142     217     640     142 
   Size:         75     423    3194

  Fragments arranged by size: 

                3194     423      75
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    598     868    1747    2239    2537     598 
   Size:        270     879     492     298    1753

  Fragments arranged by size: 

                1753     879     492     298     270
 
BsaI GGTCTCn'nnnn_

Cuts at:   3396    3396 
   Size:       3692
 
BsaAI yAC'GTr

Cuts at:    459    1045     459 
   Size:        586    3106
 
BsaBI GATnn'nnATC

Cuts at:      6    1206    3243       6 
   Size:       1200    2037     455

  Fragments arranged by size: 

                2037    1200     455
 
BsaHI Gr'CG_yC

Cuts at:    116    1123    1371    2073    3569     116 
   Size:       1007     248     702    1496     239

  Fragments arranged by size: 

                1496    1007     702     248     239
 
BsaWI w'CCGG_w

Cuts at:    664     881    1402    2080    2354    2501    2912     664 
   Size:        217     521     678     274     147     411    1444

  Fragments arranged by size: 

                1444     678     521     411     274     217     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    190     220     190 
   Size:         30    3662
 
BseYI C'CCAG_C

Cuts at:    111     135    1117    1875    2452    2948     111 
   Size:         24     982     758     577     496     855

  Fragments arranged by size: 

                 982     855     758     577     496      24
 
BsiEI CG_ry'CG

Cuts at:    104     603    1234    1280    2488     104 
   Size:        499     631      46    1208    1308

  Fragments arranged by size: 

                1308    1208     631     499      46
 
BsiHKAI G_wGCw'C

Cuts at:    316     706    1484    1674    2466     316 
   Size:        390     778     190     792    1542

  Fragments arranged by size: 

                1542     792     778     390     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    172     172 
   Size:       3692
 
Bsp1286I G_dGCh'C

Cuts at:    160     188     253     316     706    1317    1410    1484    1674 
   Size:         28      65      63     390     611      93      74     190

Cuts at:   1674    1736    2466     160 
   Size:         62     730    1386

  Fragments arranged by size: 

                1386     730     611     390     190      93      74      65
                  63      62      28
 
BspEI T'CCGG_A

Cuts at:    664    2912     664 
   Size:       2248    1444
 
BspHI T'CATG_A

Cuts at:   2868    2868 
   Size:       3692
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    742     956    1258    1639     742 
   Size:        214     302     381    2795

  Fragments arranged by size: 

                2795     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     624    1984    2038    3522    3687      50 
   Size:        574    1360      54    1484     165      55

  Fragments arranged by size: 

                1484    1360     574     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1604    1604 
   Size:       3692
 
BsrFI r'CCGG_y

Cuts at:    516    1690    1871    2080     516 
   Size:       1174     181     209    2128

  Fragments arranged by size: 

                2128    1174     209     181
 
BssSI C'ACGA_G

Cuts at:    322    1963    2321     322 
   Size:       1641     358    1693

  Fragments arranged by size: 

                1693    1641     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1069    1069 
   Size:       3692
 
BstBI TT'CG_AA

Cuts at:   2053    2053 
   Size:       3692
 
BstEII G'GTnAC_C

Cuts at:    538     538 
   Size:       3692
 
BstXI CCAn_nnnn'nTGG

Cuts at:    195     195 
   Size:       3692
 
BstYI r'GATC_y

Cuts at:      1     437     717    1184    1207    1542    1788    2800    2886 
   Size:        436     280     467      23     335     246    1012      86

Cuts at:   2886    2898    3244       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     467     449     436     346     335     280     246
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    165     245     165 
   Size:         80    3612
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    405    1642    1823    1986     405 
   Size:       1237     181     163    2111

  Fragments arranged by size: 

                2111    1237     181     163
 
BtsI GCAGTG_nn'

Cuts at:    251     935     251 
   Size:        684    3008
 
DraI TTT'AAA

Cuts at:    691     691 
   Size:       3692
 
DrdI GACnn_nn'nnGTC

Cuts at:   1398    2256    1398 
   Size:        858    2834
 
EaeI y'GGCC_r

Cuts at:    514    1277    1451    1842    1869    3214     514 
   Size:        763     174     391      27    1345     992

  Fragments arranged by size: 

                1345     992     763     391     174      27
 
EagI C'GGCC_G

Cuts at:   1277    1277 
   Size:       3692
 
EarI CTCTTCn'nnn_

Cuts at:   1715    1925    1715 
   Size:        210    3482
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1900    2210    2356    3502    1900 
   Size:        310     146    1146    2090

  Fragments arranged by size: 

                2090    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    704     704 
   Size:       3692
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    142     217     640    1517    1949    2696    2964     142 
   Size:         75     423     877     432     747     268     870

  Fragments arranged by size: 

                 877     870     747     432     423     268      75
 
EcoO109I rG'GnC_Cy

Cuts at:    831     831 
   Size:       3692
 
EcoRI G'AATT_C

Cuts at:    696     696 
   Size:       3692
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1711    1711 
   Size:       3692
 
FspI TGC'GCA

Cuts at:   1473    1473 
   Size:       3692
 
HaeII r_GCGC'y

Cuts at:    287    1126    1374    2396    3375     287 
   Size:        839     248    1022     979     604

  Fragments arranged by size: 

                1022     979     839     604     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    327     359     566     598    1534    1564    1566    1596     327 
   Size:         32     207      32     936      30       2      30    2423

  Fragments arranged by size: 

                2423     936     207      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    731     731 
   Size:       3692
 
KasI G'GCGC_C

Cuts at:   1122    1370    1122 
   Size:        248    3444
 
KpnI G_GTAC'C

Cuts at:    712     712 
   Size:       3692
 
MluI A'CGCG_T

Cuts at:   3125    3125 
   Size:       3692
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    251     263    2363    2547     251 
   Size:         12    2100     184    1396

  Fragments arranged by size: 

                2100    1396     184      12
 
MscI TGG'CCA

Cuts at:   1453    1453 
   Size:       3692
 
MslI CAynn'nnrTG

Cuts at:    268     490    1681    1808     268 
   Size:        222    1191     127    2152

  Fragments arranged by size: 

                2152    1191     222     127
 
MspA1I CmG'CkG

Cuts at:    167     803    1117    1477    2490    2735     167 
   Size:        636     314     360    1013     245    1124

  Fragments arranged by size: 

                1124    1013     636     360     314     245
 
NaeI GCC'GGC

Cuts at:   1873    1873 
   Size:       3692
 
NarI GG'CG_CC

Cuts at:   1123    1371    1123 
   Size:        248    3444
 
NgoMIV G'CCGG_C

Cuts at:   1871    1871 
   Size:       3692
 
NspI r_CATG'y

Cuts at:    745    1245    2152     745 
   Size:        500     907    2285

  Fragments arranged by size: 

                2285     907     500
 
PasI CC'CwG_GG

Cuts at:    258     258 
   Size:       3692
 
PciI A'CATG_T

Cuts at:   2148    2148 
   Size:       3692
 
PfoI T'CCnGG_A

Cuts at:    119     119 
   Size:       3692
 
PmeI GTTT'AAAC

Cuts at:    691     691 
   Size:       3692
 
PmlI CAC'GTG

Cuts at:    459     459 
   Size:       3692
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2831    2863    2831 
   Size:         32    3660
 
PspOMI G'GGCC_C

Cuts at:    156     156 
   Size:       3692
 
PstI C_TGCA'G

Cuts at:    739    1424     739 
   Size:        685    3007
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3692
 
PvuII CAG'CTG

Cuts at:   1117    1477    1117 
   Size:        360    3332
 
RsrII CG'GwC_CG

Cuts at:   1887    1887 
   Size:       3692
 
SacI G_AGCT'C

Cuts at:    706     706 
   Size:       3692
 
SacII CC_GC'GG

Cuts at:    168     168 
   Size:       3692
 
SalI G'TCGA_C

Cuts at:    729     729 
   Size:       3692
 
SapI GCTCTTCn'nnn_

Cuts at:   1715    1925    1715 
   Size:        210    3482
 
SbfI CC_TGCA'GG

Cuts at:    739     739 
   Size:       3692
 
ScaI AGT'ACT

Cuts at:    652    3209     652 
   Size:       2557    1135
 
SexAI A'CCwGG_T

Cuts at:    368     368 
   Size:       3692
 
SfcI C'TryA_G

Cuts at:     33     441     735    1420    2413    2604      33 
   Size:        408     294     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     408     294     191
 
SfoI GGC'GCC

Cuts at:   1124    1372    1124 
   Size:        248    3444
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3692
 
SmaI CCC'GGG

Cuts at:    556     714     556 
   Size:        158    3534
 
SmlI C'TyrA_G

Cuts at:    613     847    1762    2254    2516     613 
   Size:        234     915     492     262    1789

  Fragments arranged by size: 

                1789     915     492     262     234
 
SpeI A'CTAG_T

Cuts at:     67     375      67 
   Size:        308    3384
 
SphI G_CATG'C

Cuts at:    745    1245     745 
   Size:        500    3192
 
StyI C'CwwG_G

Cuts at:    287     826    1180     287 
   Size:        539     354    2799

  Fragments arranged by size: 

                2799     539     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    617    1275    1408    1867    2076     617 
   Size:        658     133     459     209    2233

  Fragments arranged by size: 

                2233     658     459     209     133
 
TatI w'GTAC_w

Cuts at:    650    3207     650 
   Size:       2557    1135
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    190     222     313     345     659     691    3209    3241     190 
   Size:         32      91      32     314      32    2518      32     641

  Fragments arranged by size: 

                2518     641     314      91      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:   1489    1489 
   Size:       3692
 
XbaI T'CTAG_A

Cuts at:    723     723 
   Size:       3692
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1187    1187 
   Size:       3692
 
XmaI C'CCGG_G

Cuts at:    554     712     554 
   Size:        158    3534
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AlfI    AlwNI
     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BcgI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
   NgoMIV     NspI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
   PspOMI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AloI     AscI
    AvrII     BaeI     BbsI    BbvCI    BfrBI     BmtI   Bpu10I    BseRI
     BsgI    BsiWI     BsmI    BsrGI   BssHII  BstZ17I   Bsu36I     ClaI
    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI
     MfeI     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI
    PflMI    PpuMI    PshAI     PsiI    PspXI     PsrI    SanDI     SfiI
    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     XmnI
     ZraI
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