Restriction map for pF1KB5419
##################################
# NO CUT ENZYMES (  167 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BamHI       BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmI        BsoBI       Bsp1286I    BspDI       BspEI       
BspLU11I    BspMI       BsrBI       BsrFI       BsrI        
BssHII      BssSI       BstAPI      BstBI       BstUI       
BstYI       Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       HapII       
HgaI        Hin1I       HincII      HindII      HpaI        
HpaII       KasI        Ksp632I     KspI        MfeI        
MflI        MluI        MroI        MspI        MunI        
MvnI        NaeI        NarI        NciI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
SwaI        TaqI        Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

Acc65I        774  GGTACC               G^GTACC
AccI          532  GTATAC               GT^[AC][GT]AC
AciI          330  GCGG                 CCGC(-3/-1)
AlwI          885  GATCC                GGATC(4/5)
Asp718I       774  GGTACC               G^GTACC
AvaII         318  GGACC                G^G[AT]CC
BanI          774  GGTACC               G^G[CT][GA]CC
BfaI          160  CTAG                 C^TAG
BsaBI         555  GATTTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiYI         433  CCCATGTTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          433  CCCATGTTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         810  GTCCC                GGGAC(10/14)
Bsp1407I      791  TGTACA               T^GTACA
BspHI         512  TCATGA               T^CATGA
BsrDI         174  CATTGC               GCAATG(2/0)
BsrGI         791  TGTACA               T^GTACA
Bst1107I      532  GTATAC               GTA^TAC
BstEII        360  GGTCACC              G^GT[ACGT]ACC
BstPI         360  GGTCACC              G^GT[ACGT]ACC
BstXI         308  CCATCGCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       532  GTATAC               GTA^TAC
DpnII         885  GATC                 ^GATC
DrdI          681  GACGGCTAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI           50  CCATGG               C^C[GA][CT]GG
EcoO65I       360  GGTCACC              G^GT[ACGT]ACC
EcoRI         945  GAATTC               G^AATTC
EcoT14I        50  CCATGG               C^C[AT][AT]GG
FauI          330  GCGGG                CCCGC(4/6)
HindIII       337  AAGCTT               A^AGCTT
KpnI          774  GGTACC               GGTAC^C
MaeI          160  CTAG                 C^TAG
MaeII          89  ACGT                 A^CGT
MamI          555  GATTTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI          885  GATC                 ^GATC
MseI          764  TTAA                 T^TAA
MspA1I        243  CAGCTG               C[AC]G^C[GT]G
NcoI           50  CCATGG               C^CATGG
NdeII         885  GATC                 ^GATC
NlaIV         774  GGTACC               GG[ACGT]^[ACGT]CC
PleI           68  GACTC                GAGTC(4/5)
PstI          127  CTGCAG               CTGCA^G
PvuII         243  CAGCTG               CAG^CTG
RcaI          512  TCATGA               T^CATGA
Sau3AI        885  GATC                 ^GATC
SspBI         791  TGTACA               T^GTACA
StyI           50  CCATGG               C^C[AT][AT]GG
TfiI          919  GATTC                G^A[AT]TC
Tru9I         764  TTAA                 T^TAA
TspRI          15  CACTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AcsI           56  AAATTT               [GA]^AATT[CT]
              945  GAATTC              
ApoI           56  AAATTT               [GA]^AATT[CT]
              945  GAATTC              
BbvI          245  GCTGC                GCAGC(8/12)
              729  GCTGC               
BsmAI         649  GAGAC                GTCTC(1/5)
              154  GTCTC               
CfoI           74  GCGC                 GCG^C
              581  GCGC                
Fnu4HI        245  GCTGC                GC^[ACGT]GC
              729  GCTGC               
HhaI           74  GCGC                 GCG^C
              581  GCGC                
HinP1I         74  GCGC                 G^CGC
              581  GCGC                
HinfI          68  GACTC                G^A[ACGT]TC
              919  GATTC               
ItaI          245  GCTGC                GC^[ACGT]GC
              729  GCTGC               
MboII         374  GAAGA                GAAGA(8/7)
              942  GAAGA               
SfcI          127  CTGCAG               C^T[GA][CT]AG
              240  CTACAG              
TseI          245  GCTGC                G^C[AT]GC
              729  GCTGC               
Tsp45I        361  GTCAC                ^GT[GC]AC
              481  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AfaI        AluI        BalI        BsaJI       BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DdeI        
EaeI        EcoRII      FokI        HaeIII      HphI        
MaeIII      MluNI       MnlI        MscI        MslI        
MvaI        MwoI        NlaIII      RsaI        Sau96I      
ScrFI       SfaNI       Tsp509I     
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