Restriction map for pF1KB4959
##################################
# NO CUT ENZYMES (   96 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AflIII      
AgeI        AhdI        ApaI        AscI        AseI        
AsnI        Asp718I     AspEI       AvaI        AviII       
AvrII       BalI        BamHI       BbeI        BbvCI       
BclI        BfrI        BlnI        BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsoBI       BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
Bst1107I    BstEII      BstPI       BstXI       BstZ17I     
ClaI        CpoI        EagI        Eam1105I    EclXI       
Eco52I      EcoO65I     EcoRI       EcoRV       FbaI        
FseI        FspI        HindIII     HpaI        HphI        
KasI        KpnI        KspI        MfeI        MluI        
MluNI       MroI        MscI        MunI        NarI        
NcoI        NdeI        NheI        NotI        PacI        
PaeR7I      PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SbfI        ScaI        SfoI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspI        SwaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   63 enzymes) #
###################################

AatII        1514  GACGTC               GACGT^C
AccI         1372  GTCTAC               GT^[AC][GT]AC
AcsI          326  AAATTC               [GA]^AATT[CT]
AocI          639  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       704  AGCGCT               AGC^GCT
ApoI          326  AAATTC               [GA]^AATT[CT]
AspI         1308  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanI          206  GGCACC               G^G[CT][GA]CC
BanII         243  GAGCTC               G[GA]GC[CT]^C
BbrPI         493  CACGTG               CAC^GTG
BciVI        1680  GGATAC               GTATCC(6/5)
BcnI          516  CCGGG                CC^[GC]GG
BglII         341  AGATCT               A^GATCT
BsaAI         493  CACGTG               [CT]AC^GT[GA]
BsaBI        1581  GATGAATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI         642  GAGGAG               GAGGAG(10/8)
BsmI         1531  GCATTC               GAATGC(1/-1)
Bsp1407I      262  TGTACA               T^GTACA
BsrFI        1157  GCCGGC               [GA]^CCGG[CT]
BsrGI         262  TGTACA               T^GTACA
BssHII         32  GCGCGC               G^CGCGC
BssSI         751  CACGAG               CACGAG(-5/-1)
BstBI         926  TTCGAA               TT^CGAA
Bsu36I        639  CCTGAGG              CC^T[ACGT]AGG
Cfr10I       1157  GCCGGC               [GA]^CCGG[CT]
DraI          408  TTTAAA               TTT^AAA
DraIII        169  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1404  GACGCACTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          137  CCGTGG               C^C[GA][CT]GG
EaeI         1462  TGGCCG               [CT]^GGCC[GA]
Eco47III      704  AGCGCT               AGC^GCT
Eco81I        639  CCTGAGG              CC^T[ACGT]AGG
EcoT14I      1459  CCTTGG               C^C[AT][AT]GG
EcoT22I      1529  ATGCAT               ATGCA^T
HaeII         704  AGCGCT               [GA]GCGC^[CT]
HincII        778  GTCAAC               GT[CT]^[GA]AC
HindII        778  GTCAAC               GT[CT]^[GA]AC
MamI         1581  GATGAATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI         1488  CATGCTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I       1150  CAGCTG               C[AC]G^C[GT]G
NaeI         1157  GCCGGC               GCC^GGC
NciI          516  CCGGG                CC^[GC]GG
NgoMIV       1157  GCCGGC               G^CCGGC
NruI         1266  TCGCGA               TCG^CGA
NsiI         1529  ATGCAT               ATGCA^T
NspI          526  ACATGC               [GA]CATG^[CT]
NspV          926  TTCGAA               TT^CGAA
PflFI        1308  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1218  CCAGGTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         493  CACGTG               CAC^GTG
PmlI          493  CACGTG               CAC^GTG
PvuII        1150  CAGCTG               CAG^CTG
SacI          243  GAGCTC               GAGCT^C
SexAI         170  ACCTGGT              A^CC[AT]GGT
SfiI          747  GGCCCACGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI          926  TTCGAA               TT^CGAA
SmlI          471  CTTGAG               C^T[CT][GA]AG
SspBI         262  TGTACA               T^GTACA
StuI         1134  AGGCCT               AGG^CCT
StyI         1459  CCTTGG               C^C[AT][AT]GG
Tth111I      1308  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1218  CCAGGTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1626  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AcyI           84  GACGCC               G[GA]^CG[CT]C
             1514  GACGTC              
AfaI          150  GTAC                 GT^AC
              263  GTAC                
Alw44I        656  GTGCAC               G^TGCAC
             1180  GTGCAC              
ApaLI         656  GTGCAC               G^TGCAC
             1180  GTGCAC              
Asp700I       430  GAAGAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              919  GAAATGCTTC          
BfaI         1195  CTAG                 C^TAG
             1627  CTAG                
BglI          748  GCCCACGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1014  GCCCTTCCGGC         
BlpI          240  GCTGAGC              GC^T[ACGT]AGC
             1152  GCTGAGC             
Bpu1102I      240  GCTGAGC              GC^T[ACGT]AGC
             1152  GCTGAGC             
BsaHI          84  GACGCC               G[GA]^CG[CT]C
             1514  GACGTC              
BsaI          488  GAGACC               GGTCTC(1/5)
             1228  GAGACC              
BstAPI        378  GCAGTTAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              530  GCAGCCACTGC         
BstF5I       1188  CATCC                GGATG(2/0)
             1391  GGATG               
CelII         240  GCTGAGC              GC^T[ACGT]AGC
             1152  GCTGAGC             
DraII        1220  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             1504  GGGTCCT             
EcoNI         100  CCTGAACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              783  CCTGCTGAAGG         
EcoO109I     1220  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             1504  GGGTCCT             
FauI           48  CCCGC                CCCGC(4/6)
             1111  GCGGG               
FokI         1188  CATCC                GGATG(9/13)
             1391  GGATG               
Hin1I          84  GACGCC               G[GA]^CG[CT]C
             1514  GACGTC              
MaeI         1195  CTAG                 C^TAG
             1627  CTAG                
PpuMI        1220  AGGTCCT              [GA]G^G[AT]CC[CT]
             1504  GGGTCCT             
PstI           37  CTGCAG               CTGCA^G
              565  CTGCAG              
RsaI          150  GTAC                 GT^AC
              263  GTAC                
TfiI          937  GAATC                G^A[AT]TC
             1263  GATTC               
Tsp45I       1081  GTGAC                ^GT[GC]AC
             1480  GTGAC               
XmnI          430  GAAGAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              919  GAAATGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        AlwNI       
AspHI       AvaII       BbsI        BbvI        BcgI        
BmyI        BpmI        BpuAI       BsaJI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspMI       BsrI        BssKI       BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       EarI        EcoRII      Fnu4HI      
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       ItaI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MspI        MvaI        MvnI        MwoI        
NdeII       NlaIII      NlaIV       PleI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
TaqI        Tru9I       TseI        Tsp509I     TspRI       
TthHB8I     XhoII       
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