Restriction map for pF1KB4568
##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AvaII       AviII       AvrII       BbrPI       BbvCI       
BcgI        BciVI       BclI        BcnI        BfaI        
BfrI        BlnI        BsaAI       BsaBI       BsaI        
BsaWI       BseAI       BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrGI       
BssSI       Bst1107I    BstAPI      BstBI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        DraIII      
DrdI        Eam1105I    EarI        Eco47III    Eco81I      
EcoNI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
KpnI        Ksp632I     MaeI        MamI        MfeI        
MluI        MroI        MunI        NciI        NcoI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PstI        PvuI        RcaI        RsrII       SalI        
SapI        SbfI        ScaI        SexAI       SfcI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AclI          416  AACGTT               AA^CGTT
AcsI          778  GAATTC               [GA]^AATT[CT]
ApoI          778  GAATTC               [GA]^AATT[CT]
AseI          295  ATTAAT               AT^TAAT
AsnI          295  ATTAAT               AT^TAAT
BamHI         266  GGATCC               G^GATCC
BbsI          244  GTCTTC               GAAGAC(2/6)
BglII         289  AGATCT               A^GATCT
BlpI         1012  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I     1012  GCTAAGC              GC^T[ACGT]AGC
BpuAI         244  GTCTTC               GAAGAC(2/6)
BsmAI         771  GTCTC                GTCTC(1/5)
BstEII        645  GGTAACC              G^GT[ACGT]ACC
BstPI         645  GGTAACC              G^GT[ACGT]ACC
BstXI         974  CCAAGAATATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        1012  GCTAAGC              GC^T[ACGT]AGC
DraII          50  GGGGCCT              [GA]G^G[ACGT]CC[CT]
DsaI            7  CCGCGG               C^C[GA][CT]GG
EcoO109I       50  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       645  GGTAACC              G^GT[ACGT]ACC
EcoRI         778  GAATTC               G^AATTC
EcoT14I      1040  CCAAGG               C^C[AT][AT]GG
KspI            7  CCGCGG               CCGC^GG
MaeII         417  ACGT                 A^CGT
NdeI          797  CATATG               CA^TATG
NspI          929  GCATGC               [GA]CATG^[CT]
PshBI         295  ATTAAT               AT^TAAT
Psp1406I      416  AACGTT               AA^CGTT
SacII           7  CCGCGG               CCGC^GG
SfiI          199  GGCCGTCCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI          929  GCATGC               GCATG^C
StyI         1040  CCAAGG               C^C[AT][AT]GG


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AfaI          492  GTAC                 GT^AC
              669  GTAC                
AspHI         153  GAGCTC               G[AT]GC[AT]^C
              339  GAGCTC              
BanII         153  GAGCTC               G[GA]GC[CT]^C
              339  GAGCTC              
BbeI          185  GGCGCC               GGCGC^C
              461  GGCGCC              
BmyI          153  GAGCTC               G[AGT]GC[ACT]^C
              339  GAGCTC              
BsgI          193  CTGCAC               GTGCAG(16/14)
              952  CTGCAC              
BsiHKAI       153  GAGCTC               G[AT]GC[AT]^C
              339  GAGCTC              
BsiYI         270  CCAGGAGGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              466  CCAATTACAGG         
BslI          270  CCAGGAGGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              466  CCAATTACAGG         
Bsp1286I      153  GAGCTC               G[AGT]GC[ACT]^C
              339  GAGCTC              
BsrFI         188  GCCGGC               [GA]^CCGG[CT]
              238  GCCGGC              
BssHII        108  GCGCGC               G^CGCGC
              110  GCGCGC              
BstYI         266  GGATCC               [GA]^GATC[CT]
              289  AGATCT              
Cfr10I        188  GCCGGC               [GA]^CCGG[CT]
              238  GCCGGC              
HapII         189  CCGG                 C^CGG
              239  CCGG                
HgaI           39  GACGC                GACGC(5/10)
              242  GCGTC               
HpaII         189  CCGG                 C^CGG
              239  CCGG                
HphI          429  GGTGA                GGTGA(8/7)
              600  GGTGA               
KasI          185  GGCGCC               G^GCGCC
              461  GGCGCC              
MflI          266  GGATCC               [GA]^GATC[CT]
              289  AGATCT              
MspI          189  CCGG                 C^CGG
              239  CCGG                
NaeI          188  GCCGGC               GCC^GGC
              238  GCCGGC              
NarI          185  GGCGCC               GG^CGCC
              461  GGCGCC              
NgoMIV        188  GCCGGC               G^CCGGC
              238  GCCGGC              
PleI          347  GAGTC                GAGTC(4/5)
              769  GAGTC               
PvuII         547  CAGCTG               CAG^CTG
             1080  CAGCTG              
RsaI          492  GTAC                 GT^AC
              669  GTAC                
SacI          153  GAGCTC               GAGCT^C
              339  GAGCTC              
SfaNI         577  GATGC                GCATC(5/9)
              753  GCATC               
SfoI          185  GGCGCC               GGC^GCC
              461  GGCGCC              
TfiI          397  GATTC                G^A[AT]TC
              802  GAATC               
Tsp509I       468  AATT                 ^AATT
              779  AATT                
XhoII         266  GGATCC               [GA]^GATC[CT]
              289  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AlwI        
BalI        BanI        BbvI        BglI        BpmI        
BsaHI       BsaJI       BseRI       BsiEI       BsmFI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
EaeI        EagI        EclXI       Eco52I      EcoRII      
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HhaI        Hin1I       HinP1I      HinfI       ItaI        
MaeIII      MboI        MboII       MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MvaI        
MvnI        MwoI        NdeII       NlaIII      NlaIV       
Sau3AI      Sau96I      ScrFI       Tru9I       TseI        
Tsp45I      TspRI       
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