Restriction map for
pF1KB3573
(Circular) (Six-base) MAPSORT of: pF1KB3573.seq Check: 332 from: 1 to: 4721
pF1KB3573 4721 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 1418 1436 1759 1418
Size: 18 323 4380
Fragments arranged by size:
4380 323 18
Acc65I G'GTAC_C
Cuts at: 328 585 1160 1737 328
Size: 257 575 577 3312
Fragments arranged by size:
3312 577 575 257
AclI AA'CG_TT
Cuts at: 4561 4561
Size: 4721
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 318 575 648 1476 2546 2978 3725 3993
Size: 134 257 73 828 1070 432 747 268
Cuts at: 3993 184
Size: 912
Fragments arranged by size:
1070 912 828 747 432 268 257 134
73
AflIII A'CryG_T
Cuts at: 1451 2073 3177 4154 1451
Size: 622 1104 977 2018
Fragments arranged by size:
2018 1104 977 622
AgeI A'CCGG_T
Cuts at: 3109 3109
Size: 4721
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 433 433
Size: 4721
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 431 463 431
Size: 32 4689
AlwNI CAG_nnn'CTG
Cuts at: 487 758 969 2026 3593 487
Size: 271 211 1057 1567 1615
Fragments arranged by size:
1615 1567 1057 271 211
ApaI G_GGCC'C
Cuts at: 389 389
Size: 4721
ApaLI G'TGCA_C
Cuts at: 3491 3491
Size: 4721
ApoI r'AATT_y
Cuts at: 528 1725 4074 4330 528
Size: 1197 2349 256 919
Fragments arranged by size:
2349 1197 919 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4721
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4721
AvaI C'yCGr_G
Cuts at: 1741 1741
Size: 4721
AvrII C'CTAG_G
Cuts at: 1014 1194 1014
Size: 180 4541
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1144 1177 1144
Size: 33 4688
BamHI G'GATC_C
Cuts at: 1644 1746 1644
Size: 102 4619
BanI G'GyrC_C
Cuts at: 223 328 585 1160 1737 2151 2399 2434 223
Size: 105 257 575 577 414 248 35 2510
Fragments arranged by size:
2510 577 575 414 257 248 105 35
BanII G_rGCy'C
Cuts at: 389 717 1258 1516 1735 2765 389
Size: 328 541 258 219 1030 2345
Fragments arranged by size:
2345 1030 541 328 258 219
BbeI G_GCGC'C
Cuts at: 2155 2403 2155
Size: 248 4473
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1570 1604 1570
Size: 34 4687
BciVI GTATCCnnnnn_n'
Cuts at: 946 2619 3380 4721 946
Size: 1673 761 1341 946
Fragments arranged by size:
1673 1341 946 761
BclI T'GATC_A
Cuts at: 2241 2241
Size: 4721
BfrBI ATG'CAT
Cuts at: 1575 1575
Size: 4721
BglI GCCn_nnn'nGGC
Cuts at: 1243 1243
Size: 4721
BglII A'GATC_T
Cuts at: 2236 4273 2236
Size: 2037 2684
BlpI GC'TnA_GC
Cuts at: 887 1833 887
Size: 946 3775
Bme1580I G_kGCm'C
Cuts at: 228 389 412 2346 2439 3495 228
Size: 161 23 1934 93 1056 1454
Fragments arranged by size:
1934 1454 1056 161 93 23
BmrI ACTGGGnnnn_n'
Cuts at: 1104 1933 2014 2349 4493 1104
Size: 829 81 335 2144 1332
Fragments arranged by size:
2144 1332 829 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1689 3088 1689
Size: 1399 3322
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1684 1163
Size: 521 4200
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 676 763 799 1897 2776 3268 3566 676
Size: 87 36 1098 879 492 298 1831
Fragments arranged by size:
1831 1098 879 492 298 87 36
BsaI GGTCTCn'nnnn_
Cuts at: 4425 4425
Size: 4721
BsaAI yAC'GTr
Cuts at: 2074 2074
Size: 4721
BsaBI GATnn'nnATC
Cuts at: 6 2235 4272 6
Size: 2229 2037 455
Fragments arranged by size:
2229 2037 455
BsaHI Gr'CG_yC
Cuts at: 1707 2152 2400 3102 4598 1707
Size: 445 248 702 1496 1830
Fragments arranged by size:
1830 1496 702 445 248
BsaWI w'CCGG_w
Cuts at: 1910 2431 3109 3383 3530 3941 1910
Size: 521 678 274 147 411 2690
Fragments arranged by size:
2690 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 280 310 280
Size: 30 4691
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1605 1605
Size: 4721
BseYI C'CCAG_C
Cuts at: 728 937 2146 2904 3481 3977 728
Size: 209 1209 758 577 496 1472
Fragments arranged by size:
1472 1209 758 577 496 209
BsiEI CG_ry'CG
Cuts at: 104 2263 2309 3517 104
Size: 2159 46 1208 1308
Fragments arranged by size:
2159 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 893 1735 2513 2703 3495 893
Size: 842 778 190 792 2119
Fragments arranged by size:
2119 842 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 228 389 412 717 893 1258 1516 1735 2346
Size: 161 23 305 176 365 258 219 611
Cuts at: 2346 2439 2513 2703 2765 3495 228
Size: 93 74 190 62 730 1454
Fragments arranged by size:
1454 730 611 365 305 258 219 190
176 161 93 74 62 23
BspEI T'CCGG_A
Cuts at: 3941 3941
Size: 4721
BspHI T'CATG_A
Cuts at: 3897 3897
Size: 4721
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1771 1985 2287 2668 1771
Size: 214 302 381 3824
Fragments arranged by size:
3824 381 302 214
BsrBI CCG'CTC
Cuts at: 50 793 1613 3013 3067 4551 4716 50
Size: 743 820 1400 54 1484 165 55
Fragments arranged by size:
1484 1400 820 743 165 55 54
BsrDI GCAATG_nn'
Cuts at: 285 1001 1145 2633 285
Size: 716 144 1488 2373
Fragments arranged by size:
2373 1488 716 144
BsrFI r'CCGG_y
Cuts at: 2719 2900 3109 2719
Size: 181 209 4331
Fragments arranged by size:
4331 209 181
BsrGI T'GTAC_A
Cuts at: 1152 1152
Size: 4721
BssSI C'ACGA_G
Cuts at: 2992 3350 2992
Size: 358 4363
BstAPI GCAn_nnn'nTGC
Cuts at: 223 1278 2098 223
Size: 1055 820 2846
Fragments arranged by size:
2846 1055 820
BstBI TT'CG_AA
Cuts at: 3082 3082
Size: 4721
BstYI r'GATC_y
Cuts at: 1 1644 1746 2213 2236 2571 2817 3829 3915
Size: 1643 102 467 23 335 246 1012 86
Cuts at: 3915 3927 4273 1
Size: 12 346 449
Fragments arranged by size:
1643 1012 467 449 346 335 246 102
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1508 1508
Size: 4721
BtgI C'CryG_G
Cuts at: 107 590 107
Size: 483 4238
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2671 2852 3015 2671
Size: 181 163 4377
Fragments arranged by size:
4377 181 163
BtsI GCAGTG_nn'
Cuts at: 212 363 917 1568 1964 212
Size: 151 554 651 396 2969
Fragments arranged by size:
2969 651 554 396 151
DraI TTT'AAA
Cuts at: 1720 1720
Size: 4721
DraIII CAC_nnn'GTG
Cuts at: 358 358
Size: 4721
DrdI GACnn_nn'nnGTC
Cuts at: 2427 3285 2427
Size: 858 3863
EaeI y'GGCC_r
Cuts at: 593 1485 2306 2480 2871 2898 4243 593
Size: 892 821 174 391 27 1345 1071
Fragments arranged by size:
1345 1071 892 821 391 174 27
EagI C'GGCC_G
Cuts at: 2306 2306
Size: 4721
EarI CTCTTCn'nnn_
Cuts at: 2744 2954 2744
Size: 210 4511
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1042 2929 3239 3385 4531 1042
Size: 1887 310 146 1146 1232
Fragments arranged by size:
1887 1232 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1733 1733
Size: 4721
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 318 575 648 1163 1476 1684 2546 2978
Size: 134 257 73 515 313 208 862 432
Cuts at: 2978 3725 3993 184
Size: 747 268 912
Fragments arranged by size:
912 862 747 515 432 313 268 257
208 134 73
EcoNI CCTnn'n_nnAGG
Cuts at: 673 673
Size: 4721
EcoO109I rG'GnC_Cy
Cuts at: 385 1018 1393 1860 385
Size: 633 375 467 3246
Fragments arranged by size:
3246 633 467 375
EcoRI G'AATT_C
Cuts at: 1725 1725
Size: 4721
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2740 2740
Size: 4721
FspI TGC'GCA
Cuts at: 2502 2502
Size: 4721
HaeII r_GCGC'y
Cuts at: 2155 2403 3425 4404 2155
Size: 248 1022 979 2472
Fragments arranged by size:
2472 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 560 592 2563 2593 2595 2625 560
Size: 32 1971 30 2 30 2656
Fragments arranged by size:
2656 1971 32 30 30 2
HincII GTy'rAC
Cuts at: 1419 1760 1419
Size: 341 4380
HindIII A'AGCT_T
Cuts at: 246 246
Size: 4721
KasI G'GCGC_C
Cuts at: 2151 2399 2151
Size: 248 4473
KpnI G_GTAC'C
Cuts at: 332 589 1164 1741 332
Size: 257 575 577 3312
Fragments arranged by size:
3312 577 575 257
MfeI C'AATT_G
Cuts at: 1398 1398
Size: 4721
MluI A'CGCG_T
Cuts at: 4154 4154
Size: 4721
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3392 3576 3392
Size: 184 4537
MscI TGG'CCA
Cuts at: 595 1487 2482 595
Size: 892 995 2834
Fragments arranged by size:
2834 995 892
MslI CAynn'nnrTG
Cuts at: 1046 1580 2710 2837 1046
Size: 534 1130 127 2930
Fragments arranged by size:
2930 1130 534 127
MspA1I CmG'CkG
Cuts at: 599 919 1832 2146 2506 3519 3764 599
Size: 320 913 314 360 1013 245 1556
Fragments arranged by size:
1556 1013 913 360 320 314 245
NaeI GCC'GGC
Cuts at: 2902 2902
Size: 4721
NarI GG'CG_CC
Cuts at: 2152 2400 2152
Size: 248 4473
NcoI C'CATG_G
Cuts at: 107 590 107
Size: 483 4238
NgoMIV G'CCGG_C
Cuts at: 2900 2900
Size: 4721
NsiI A_TGCA'T
Cuts at: 1577 1577
Size: 4721
NspI r_CATG'y
Cuts at: 1774 2274 3181 1774
Size: 500 907 3314
Fragments arranged by size:
3314 907 500
PciI A'CATG_T
Cuts at: 3177 3177
Size: 4721
PflMI CCAn_nnn'nTGG
Cuts at: 758 969 1265 1524 758
Size: 211 296 259 3955
Fragments arranged by size:
3955 296 259 211
PfoI T'CCnGG_A
Cuts at: 872 1661 872
Size: 789 3932
PmeI GTTT'AAAC
Cuts at: 1720 1720
Size: 4721
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3860 3892 3860
Size: 32 4689
PpuMI rG'GwC_Cy
Cuts at: 1018 1393 1018
Size: 375 4346
PshAI GACnn'nnGTC
Cuts at: 1016 1016
Size: 4721
PspOMI G'GGCC_C
Cuts at: 385 385
Size: 4721
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1324 1356 1324
Size: 32 4689
PstI C_TGCA'G
Cuts at: 434 722 1768 2453 434
Size: 288 1046 685 2702
Fragments arranged by size:
2702 1046 685 288
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4721
PvuII CAG'CTG
Cuts at: 599 919 2146 2506 599
Size: 320 1227 360 2814
Fragments arranged by size:
2814 1227 360 320
RsrII CG'GwC_CG
Cuts at: 2916 2916
Size: 4721
SacI G_AGCT'C
Cuts at: 1735 1735
Size: 4721
SalI G'TCGA_C
Cuts at: 1417 1758 1417
Size: 341 4380
SapI GCTCTTCn'nnn_
Cuts at: 2744 2954 2744
Size: 210 4511
SbfI CC_TGCA'GG
Cuts at: 1768 1768
Size: 4721
ScaI AGT'ACT
Cuts at: 1038 4238 1038
Size: 3200 1521
SfcI C'TryA_G
Cuts at: 33 255 430 718 1764 2449 3442 3633 33
Size: 222 175 288 1046 685 993 191 1121
Fragments arranged by size:
1121 1046 993 685 288 222 191 175
SfoI GGC'GCC
Cuts at: 2153 2401 2153
Size: 248 4473
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4721
SmaI CCC'GGG
Cuts at: 1743 1743
Size: 4721
SmlI C'TyrA_G
Cuts at: 691 778 814 1876 2791 3283 3545 691
Size: 87 36 1062 915 492 262 1867
Fragments arranged by size:
1867 1062 915 492 262 87 36
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4721
SphI G_CATG'C
Cuts at: 1774 2274 1774
Size: 500 4221
StuI AGG'CCT
Cuts at: 416 1246 416
Size: 830 3891
StyI C'CwwG_G
Cuts at: 107 324 590 1014 1194 1445 1482 1855 2209
Size: 217 266 424 180 251 37 373 354
Cuts at: 2209 107
Size: 2619
Fragments arranged by size:
2619 424 373 354 266 251 217 180
37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 783 1375 1392 2304 2437 2896 3105 783
Size: 592 17 912 133 459 209 2399
Fragments arranged by size:
2399 912 592 459 209 133 17
TatI w'GTAC_w
Cuts at: 349 1036 1152 1310 4236 349
Size: 687 116 158 2926 834
Fragments arranged by size:
2926 834 687 158 116
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4238 4270 4238
Size: 32 4689
Tth111I GACn'n_nGTC
Cuts at: 2518 2518
Size: 4721
XbaI T'CTAG_A
Cuts at: 1752 1752
Size: 4721
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2216 2216
Size: 4721
XmaI C'CCGG_G
Cuts at: 1741 1741
Size: 4721
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AscI BbsI
BbvCI BmgBI BmtI Bpu10I BsgI BsiWI BsmI BsmBI
BssHII BstEII BstXI BstZ17I ClaI CspCI EcoRV FseI
FspAI HpaI NdeI NheI NotI NruI PacI PasI
PmlI PsiI PspXI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI