Restriction map for pF1KB3524
##################################
# NO CUT ENZYMES (  144 enzymes) #
##################################

Acc65I      AccIII      AflII       AflIII      AgeI        
AhdI        Alw44I      Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspHI       AspI        AviII       AvrII       BalI        
BanI        BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmFI       BsmI        Bsp1407I    BspDI       BspEI       
BspLU11I    BspMI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstZ17I     CelII       
Cfr10I      ClaI        CpoI        DraII       DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO109I    EcoO65I     
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HgaI        HpaI        KasI        
KpnI        KspI        MluI        MluNI       MroI        
MscI        MwoI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       PstI        PvuI        
PvuII       RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SpeI        
SphI        Sse8387I    SspBI       StuI        SwaI        
TaqI        Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AatII         452  GACGTC               GACGT^C
AccI          308  GTCTAC               GT^[AC][GT]AC
AccII          97  CGCG                 CG^CG
AclI          748  AACGTT               AA^CGTT
AcyI          452  GACGTC               G[GA]^CG[CT]C
Asp700I      1087  GAAGATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI           79  CTCGGG               C^[CT]CG[GA]G
AvaII         203  GGTCC                G^G[AT]CC
BamHI         705  GGATCC               G^GATCC
BbsI         1366  GAAGAC               GAAGAC(2/6)
BcnI          206  CCGGG                CC^[GC]GG
BpuAI        1366  GAAGAC               GAAGAC(2/6)
BsaAI        1187  TACGTA               [CT]AC^GT[GA]
BsaBI         946  GATCCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         452  GACGTC               G[GA]^CG[CT]C
BsmAI         125  GAGAC                GTCTC(1/5)
BsmBI         125  GAGACG               CGTCTC(1/5)
BsoBI          79  CTCGGG               C^[CT]CG[GA]G
BsrBI         362  GAGCGG               CCGCTC(-3/-3)
BstNI         835  CCTGG                CC^[AT]GG
BstUI          97  CGCG                 CG^CG
CfoI           96  GCGC                 GCG^C
Cfr13I        203  GGTCC                G^G[ACGT]CC
DraI          741  TTTAAA               TTT^AAA
EcoRI         716  GAATTC               G^AATTC
EcoRII        835  CCTGG                ^CC[AT]GG
EcoT14I      1097  CCAAGG               C^C[AT][AT]GG
HapII         206  CCGG                 C^CGG
HhaI           96  GCGC                 GCG^C
Hin1I         452  GACGTC               G[GA]^CG[CT]C
HinP1I         96  GCGC                 G^CGC
HincII        903  GTTGAC               GT[CT]^[GA]AC
HindII        903  GTTGAC               GT[CT]^[GA]AC
HpaII         206  CCGG                 C^CGG
MamI          946  GATCCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1319  CAATTG               C^AATTG
MslI          580  CATGAATATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          206  CCGG                 C^CGG
MunI         1319  CAATTG               C^AATTG
MvaI          835  CCTGG                CC^[AT]GG
MvnI           97  CGCG                 CG^CG
NciI          206  CCGGG                CC^[GC]GG
NheI          933  GCTAGC               G^CTAGC
Psp1406I      748  AACGTT               AA^CGTT
Sau96I        203  GGTCC                G^G[ACGT]CC
SmlI         1351  CTTGAG               C^T[CT][GA]AG
SnaBI        1187  TACGTA               TAC^GTA
SspI          884  AATATT               AAT^ATT
StyI         1097  CCAAGG               C^C[AT][AT]GG
TspRI        1235  CACTG                CA[GC]TG[ACGT][ACGT]^
XmnI         1087  GAAGATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AlwNI         509  CAGACTCTG            CAG[ACGT][ACGT][ACGT]^CTG
              830  CAGATCCTG           
AocI          387  CCTGAGG              CC^T[ACGT]AGG
              709  CCTAAGG             
BanII          76  GGGCTC               G[GA]GC[CT]^C
              841  GAGCCC              
BmyI           76  GGGCTC               G[AGT]GC[ACT]^C
              841  GAGCCC              
BpmI           91  CTGGAG               CTGGAG(16/14)
              680  CTGGAG              
BsaJI         206  CCGGGG               C^C[ACGT][ACGT]GG
             1097  CCAAGG              
BsiYI         110  CCGCTGCTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              955  CCCTATAGGGG         
BslI          110  CCGCTGCTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              955  CCCTATAGGGG         
Bsp1286I       76  GGGCTC               G[AGT]GC[ACT]^C
              841  GAGCCC              
BspHI         399  TCATGA               T^CATGA
              543  TCATGA              
BssKI         206  CCGGG                ^CC[ACGT]GG
              835  CCTGG               
Bsu36I        387  CCTGAGG              CC^T[ACGT]AGG
              709  CCTAAGG             
Cac8I         839  GCGAGC               GC[ACGT]^[ACGT]GC
              933  GCTAGC              
EaeI            2  TGGCCG               [CT]^GGCC[GA]
              365  CGGCCA              
Eco81I        387  CCTGAGG              CC^T[ACGT]AGG
              709  CCTAAGG             
HaeIII          3  GGCC                 GG^CC
              366  GGCC                
MspA1I        110  CCGCTG               C[AC]G^C[GT]G
              155  CCGCTG              
NlaIV         202  GGGTCC               GG[ACGT]^[ACGT]CC
              705  GGATCC              
RcaI          399  TCATGA               T^CATGA
              543  TCATGA              
ScrFI         206  CCGGG                CC^[ACGT]GG
              835  CCTGG               
SfcI          482  CTACAG               C^T[GA][CT]AG
              957  CTATAG              
Tsp45I       1021  GTCAC                ^GT[GC]AC
             1178  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   41 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        BbvI        BfaI        BseRI       BsrI        
BstF5I      BstYI       DdeI        DpnII       EarI        
Fnu4HI      FokI        HindIII     HinfI       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
NdeII       NlaIII      PleI        RsaI        Sau3AI      
SfaNI       TfiI        Tru9I       TseI        Tsp509I     
XhoII       
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